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CD117 antibody

RRID:AB_11154233

Antibody ID

AB_11154233

Target Antigen

CD117 mouse

Proper Citation

(BD Biosciences Cat# 562417, RRID:AB_11154233)

Clonality

monoclonal antibody

Comments

Flow cytometry

Host Organism

rat

Vendor

BD Biosciences Go To Vendor

Cat Num

562417

Publications that use this research resource

A polymorphism in the tumor suppressor p53 affects aging and longevity in mouse models.

  • Zhao Y
  • Elife
  • 2018 Mar 20

Literature context:


Abstract:

Tumor suppressor p53 prevents early death due to cancer development. However, the role of p53 in aging process and longevity has not been well-established. In humans, single nucleotide polymorphism (SNP) with either arginine (R72) or proline (P72) at codon 72 influences p53 activity; the P72 allele has a weaker p53 activity and function in tumor suppression. Here, employing a mouse model with knock-in of human TP53 gene carrying codon 72 SNP, we found that despite increased cancer risk, P72 mice that escape tumor development display a longer lifespan than R72 mice. Further, P72 mice have a delayed development of aging-associated phenotypes compared with R72 mice. Mechanistically, P72 mice can better retain the self-renewal function of stem/progenitor cells compared with R72 mice during aging. This study provides direct genetic evidence demonstrating that p53 codon 72 SNP directly impacts aging and longevity, which supports a role of p53 in regulation of longevity.

Funding information:
  • Lawrence Ellison Foundation - New Investigate Award AG-NS-0781-11()
  • National Institutes of Health - 1R01CA160558()
  • National Institutes of Health - 1R01CA203965()
  • National Institutes of Health - 1R01CA227912()
  • National Institutes of Health - F99CA222734()
  • NCI NIH HHS - F99 CA222734()
  • New Jersey Commission on Cancer Research - Postdoctoral Fellowship Award()
  • NICHD NIH HHS - R37HD033082(United States)
  • U.S. Department of Defense - W81XWH-16-1-0358()

Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions.

  • Evrard M
  • Immunity
  • 2018 Feb 20

Literature context:


Abstract:

Neutrophils are specialized innate cells that require constant replenishment from proliferative bone marrow (BM) precursors as a result of their short half-life. Although it is established that neutrophils are derived from the granulocyte-macrophage progenitor (GMP), the differentiation pathways from GMP to functional mature neutrophils are poorly defined. Using mass cytometry (CyTOF) and cell-cycle-based analysis, we identified three neutrophil subsets within the BM: a committed proliferative neutrophil precursor (preNeu) which differentiates into non-proliferating immature neutrophils and mature neutrophils. Transcriptomic profiling and functional analysis revealed that preNeu require the C/EBPε transcription factor for their generation from the GMP, and their proliferative program is substituted by a gain of migratory and effector function as they mature. preNeus expand under microbial and tumoral stress, and immature neutrophils are recruited to the periphery of tumor-bearing mice. In summary, our study identifies specialized BM granulocytic populations that ensure supply under homeostasis and stress responses.

Funding information:
  • NICHD NIH HHS - L50 HD073094(United States)