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Anti-DYKDDDDK Affinity Gel (Binds the same epitope as Sigma's Anti-FLAG M2 Antibody) - 200-350-383


Antibody ID


Target Antigen

Anti-DYKDDDDK (FLAG tag) Affinity Gel carboxy and amino terminal linked flag&trade, tagged recombinant proteins

Proper Citation

(Rockland Cat# 200-350-383, RRID:AB_10704031)


monoclonal antibody


Immunoprecipitation, Anti-DYKDDDDK Affinity Gel is optimally suited for immunoprecipitation and purification of FLAG tagged fusion proteins

Clone ID


Host Organism




Cat Num


Publications that use this research resource

The complex of TRIP-Br1 and XIAP ubiquitinates and degrades multiple adenylyl cyclase isoforms.

  • Hu W
  • Elife
  • 2017 Jun 28

Literature context:


Adenylyl cyclases (ACs) generate cAMP, a second messenger of utmost importance that regulates a vast array of biological processes in all kingdoms of life. However, almost nothing is known about how AC activity is regulated through protein degradation mediated by ubiquitination or other mechanisms. Here, we show that transcriptional regulator interacting with the PHD-bromodomain 1 (TRIP-Br1, Sertad1), a newly identified protein with poorly characterized functions, acts as an adaptor that bridges the interaction of multiple AC isoforms with X-linked inhibitor of apoptosis protein (XIAP), a RING-domain E3 ubiquitin ligase. XIAP ubiquitinates a highly conserved Lys residue in AC isoforms and thereby accelerates the endocytosis and degradation of multiple AC isoforms in human cell lines and mice. XIAP/TRIP-Br1-mediated degradation of ACs forms part of a negative-feedback loop that controls the homeostasis of cAMP signaling in mice. Our findings reveal a previously unrecognized mechanism for degrading multiple AC isoforms and modulating the homeostasis of cAMP signaling.