X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Fyn Antibody

RRID:AB_10698604

Antibody ID

AB_10698604

Target Antigen

Fyn human, mouse, h, m

Vendor

Cell Signaling Technology

Cat Num

4023

Proper Citation

(Cell Signaling Technology Cat# 4023, RRID:AB_10698604)

Clonality

polyclonal antibody

Host Organism

rabbit

Comments

manufacturer recommendations: Western Blot; Western blot; The following antibodies were determined to be duplicates and consolidated by curator on 11/2018: AB_10698604, AB_10829465, AB_2108441.

Publications that use this research resource

L1 coupling to ankyrin and the spectrin-actin cytoskeleton modulates ethanol inhibition of L1 adhesion and ethanol teratogenesis.

  • Dou X
  • FASEB J.
  • 2017 Nov 8

Literature context: CA, USA); and rabbit pAb 4023S (RRID:AB_10698604) for Fyn and mAb 2110S (RRID AB


Abstract:

Ethanol causes fetal alcohol spectrum disorders (FASDs) partly by inhibiting cell adhesion mediated by the L1 neural cell adhesion molecule. Ethanol interacts with an alcohol binding pocket in the L1 extracellular domain (ECD), and dephosphorylation of S1248 in the L1 cytoplasmic domain (CD) renders L1 adhesion insensitive to inhibition by ethanol (L1 insensitive). The mechanism underlying this inside-out signaling is unknown. Here we show that phosphorylation of the human L1-CD at S1152, Y1176, S1181, and S1248 renders L1 sensitive to ethanol by promoting L1 coupling with ankyrin-G and the spectrin-actin cytoskeleton. Knockdown of ankyrin-G or L1 mutations that uncouple L1 from ankyrin reduce L1 sensitivity to ethanol, but not methanol, consistent with a small conformational change in the extracellular alcohol binding pocket. Phosphorylation of Y1176 and ankyrin-G coupling with L1 are higher in NIH/3T3 clonal cell lines in which ethanol inhibits L1 adhesion than in ethanol-resistant NIH/3T3 clonal cell lines. Similarly, phosphorylation of Y1176 is higher in C57BL/6J mice that are sensitive to ethanol teratogenesis than in ethanol resistant C57BL/6N mice. Finally, polymorphisms in genes that encode ankyrin-G and p90rsk, a kinase that phosphorylates S1152, are linked to facial dysmorphology in children with heavy prenatal ethanol exposure. These findings indicate that genes that regulate L1 coupling to ankyrin may influence susceptibility to FASD.-Dou, X., Menkari, C., Mitsuyama, R., Foroud, T., Wetherill, L., Hammond, P., Suttie, M., Chen, X., Chen, S.-Y., Charness, M. E., Collaborative Initiative on Fetal Alcohol Spectrum Disorders. L1 coupling to ankyrin and the spectrin-actin cytoskeleton modulates ethanol inhibition of L1 adhesion and ethanol teratogenesis.

Funding information:
  • NCI NIH HHS - CA70907(United States)
  • NIAAA NIH HHS - P50 AA024337()
  • NIAAA NIH HHS - R01 AA020265()
  • NIAAA NIH HHS - R01 AA021434()