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Doublecortin Antibody

RRID:AB_10693771

Antibody ID

AB_561007

Target Antigen

Doublecortin null

Vendor

Cell Signaling Technology

Cat Num

4604

Proper Citation

(Cell Signaling Technology Cat# 4604, RRID:AB_561007)

Clonality

monoclonal antibody

Host Organism

rabbit

Comments

manufacturer recommendations: Western blot,Immunoprecipitation,Immunofluorescence (Frozen),Flow cytometry; Western Blot; Immunohistochemistry; Flow Cytometry; Immunohistochemistry - frozen; Immunofluorescence; Immunoprecipitation; The following antibodies were determined to be duplicates and consolidated by curator on 10/2018: AB_10693771, AB_561007.

Publications that use this research resource

The Subventricular Zone Response to Stroke Is Not a Therapeutic Target of Anti-Nogo-A Immunotherapy.

  • Shepherd DJ
  • J. Neuropathol. Exp. Neurol.
  • 2017 Aug 1

Literature context: in (DCX) Cell Signaling 4604S [RRID:AB_10693771] 1:500


Abstract:

Ischemic stroke is a leading cause of adult disability with no pharmacological treatments to promote the recovery of lost function. Neutralizing antibodies against the neurite outgrowth inhibitor Nogo-A have emerged as a promising treatment for subacute and chronic stroke in animal models; however, whether anti-Nogo-A treatment affects poststroke neurogenesis remains poorly understood. In this study, we confirmed expression of Nogo-A by neuroblasts in the adult rat subventricular zone (SVZ), a major neurogenic niche; however, we found no evidence that Nogo-A was expressed at the surface of these cells. In vitro migration assays demonstrated that Nogo-A signaling induced a modest reduction in neuroblast migration speed, while anti-Nogo-A antibodies had no effect on motility properties. Using a permanent distal middle cerebral artery occlusion model of cortical stroke, we found that the number of proliferating cells in the SVZ was unaffected in response to stroke, while neuroblast mobilization from the SVZ toward the stroke lesion correlated positively with lesion size. However, we found no evidence that proliferation or neuroblast mobilization were affected by anti-Nogo-A antibody treatment. Our results suggest that the SVZ is not a therapeutic target of anti-Nogo-A immunotherapy, and contribute to our understanding of the SVZ response to cortical stroke.

Funding information:
  • NINDS NIH HHS - K08NS048858(United States)

Lunatic fringe-mediated Notch signaling regulates adult hippocampal neural stem cell maintenance.

  • Semerci F
  • Elife
  • 2017 Jul 12

Literature context: Signaling Technology Cat# 4604S RRID:AB_10693771) at 1:200; mouse anti-GFAP (Sig


Abstract:

Hippocampal neural stem cells (NSCs) integrate inputs from multiple sources to balance quiescence and activation. Notch signaling plays a key role during this process. Here, we report that Lunatic fringe (Lfng), a key modifier of the Notch receptor, is selectively expressed in NSCs. Further, Lfng in NSCs and Notch ligands Delta1 and Jagged1, expressed by their progeny, together influence NSC recruitment, cell cycle duration, and terminal fate. We propose a new model in which Lfng-mediated Notch signaling enables direct communication between a NSC and its descendants, so that progeny can send feedback signals to the 'mother' cell to modify its cell cycle status. Lfng-mediated Notch signaling appears to be a key factor governing NSC quiescence, division, and fate.

Funding information:
  • NCI NIH HHS - P30 CA125123()
  • NCRR NIH HHS - S10 RR024574()
  • NIAID NIH HHS - P30 AI036211()
  • NICHD NIH HHS - U54 HD083092()
  • NIDCD NIH HHS - R01 DC006185()
  • NIDCD NIH HHS - R01 DC014832()
  • NIH HHS - S10 OD016167()

Tridimensional Visualization and Analysis of Early Human Development.

  • Belle M
  • Cell
  • 2017 Mar 23

Literature context: t# 4604S; RRID:AB_10693771 Mouse mono


Abstract:

Generating a precise cellular and molecular cartography of the human embryo is essential to our understanding of the mechanisms of organogenesis in normal and pathological conditions. Here, we have combined whole-mount immunostaining, 3DISCO clearing, and light-sheet imaging to start building a 3D cellular map of the human development during the first trimester of gestation. We provide high-resolution 3D images of the developing peripheral nervous, muscular, vascular, cardiopulmonary, and urogenital systems. We found that the adult-like pattern of skin innervation is established before the end of the first trimester, showing important intra- and inter-individual variations in nerve branches. We also present evidence for a differential vascularization of the male and female genital tracts concomitant with sex determination. This work paves the way for a cellular and molecular reference atlas of human cells, which will be of paramount importance to understanding human development in health and disease. PAPERCLIP.

Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex.

  • Bifari F
  • Cell Stem Cell
  • 2017 Mar 2

Literature context: at#4604S, RRID:AB_10693771 Goat polyc


Abstract:

Whether new neurons are added in the postnatal cerebral cortex is still debated. Here, we report that the meninges of perinatal mice contain a population of neurogenic progenitors formed during embryonic development that migrate to the caudal cortex and differentiate into Satb2+ neurons in cortical layers II-IV. The resulting neurons are electrically functional and integrated into local microcircuits. Single-cell RNA sequencing identified meningeal cells with distinct transcriptome signatures characteristic of (1) neurogenic radial glia-like cells (resembling neural stem cells in the SVZ), (2) neuronal cells, and (3) a cell type with an intermediate phenotype, possibly representing radial glia-like meningeal cells differentiating to neuronal cells. Thus, we have identified a pool of embryonically derived radial glia-like cells present in the meninges that migrate and differentiate into functional neurons in the neonatal cerebral cortex.

Funding information:
  • NINDS NIH HHS - R01 NS036715(United States)

Anti-Nogo-A Immunotherapy Does Not Alter Hippocampal Neurogenesis after Stroke in Adult Rats.

  • Shepherd DJ
  • Front Neurosci
  • 2016 Nov 2

Literature context: rtin (DCX)Cell Signaling 4604S [RRID: AB_10693771]1:500Goat anti-doublecortin (DCX


Abstract:

Ischemic stroke is a leading cause of adult disability, including cognitive impairment. Our laboratory has previously shown that treatment with function-blocking antibodies against the neurite growth inhibitory protein Nogo-A promotes functional recovery after stroke in adult and aged rats, including enhancing spatial memory performance, for which the hippocampus is critically important. Since spatial memory has been linked to hippocampal neurogenesis, we investigated whether anti-Nogo-A treatment increases hippocampal neurogenesis after stroke. Adult rats were subject to permanent middle cerebral artery occlusion followed 1 week later by 2 weeks of antibody treatment. Cellular proliferation in the dentate gyrus was quantified at the end of treatment, and the number of newborn neurons was determined at 8 weeks post-stroke. Treatment with both anti-Nogo-A and control antibodies stimulated the accumulation of new microglia/macrophages in the dentate granule cell layer, but neither treatment increased cellular proliferation or the number of newborn neurons above stroke-only levels. These results suggest that anti-Nogo-A immunotherapy does not increase post-stroke hippocampal neurogenesis.

Funding information:
  • NINDS NIH HHS - NS072030(United States)