URL: http://antibodyregistry.org/AB_10541840
Proper Citation: (Enzo Life Sciences Cat# BML-PW8810, RRID:AB_10541840)
Target Antigen: Mono- and polyubiquitinylated conjugates mAb (FK2)
Clonality: monoclonal
Comments: manufacturer recommendations: IgG1 ELISA, Immunohistochemistry, Immunoprecipitation (Care must be taken as MAb to Mono- and Polyubiquitinylated Conjugates (FK2) demonstrates affinity for both free ubiquitin and multi-ubiquitinylated species when immobilised), Western Blot (1:1000), Optimal conditions must be determined individually for each application.
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No rating or validation information has been found for Mono- and polyubiquitinylated conjugates, mAb (FK2).
Possibly Non-Specific
Extracted Antibody Information: "1:300), anti-Cdu1 (1:200), anti-Ub- FK2 (Enzo Life Sciences Cat# BML-PW8810 RRID:AB_10541840;"
Extracted Specificity Statement: "Indirect immunofluorescence staining for cHSP60 (green channel) and Cdu1 (red channel) was performed. The Cdu1 antibody shows unspecific staining of nuclear structures. Scale bar 10 µm. (D) Relative amount of Mcl-1 in HeLa cells infected with Ctr WT and Tn-cdu1 mutant for 24 or 30 hr."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:28347402
Source: Antibody Registry