URL: http://antibodyregistry.org/AB_831022
Proper Citation: Santa Cruz Biotechnology Cat# sc-65670, RRID:AB_831022
Target Antigen: beta-Galactosidase(40-1a)
Host Organism: mouse
Clonality: monoclonal
Comments: validation status unknown check with seller; recommendations: Immunoprecipitation; Western Blot; ELISA; Immunofluorescence; WB, IP, IF, ELISA
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No rating or validation information has been found for beta-Galactosidase(40-1a).
Issue
This antibody recognizes the bacterial β-galactosidase protein, but not the mammalian β-galactosidase protein. It has been used by authors to detect the mammalian protein, do not do this because it will not work.
"While endogenous mammalian β-galactosidase protein is used as a marker for senescent cells, while the E. coli β-galactosidase protein is a component of the versatile LacZ reporter system used for genetics experiments. While these enzymes have the same name, catalyse the same reaction, and share a useful substrate in X-Gal, they are otherwise related to separate lines of scientific inquiry." - Sholto David (for better science blog)
Source: Antibody Registry
