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URL: http://www.addgene.org/66850
Proper Citation: RRID:Addgene_66850
Insert Name: WWTR1/TAZ
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21586534
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: I isolated NheI/MfeI fragment from pClneoEGFPC2 TAZ and subcloned into NheI/EcoRi sites of the original pLL3.7. That is the reason why T3 promtoer can be used for the sequencing. That sequence is derived from pClneo. Please note: Plasmid is missing 444bp compared to pLL3.7 backbone sequence. This removes the KS primer and loxP at 5' end of the insert, but is not expected to affect plasmid function.
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Source: Addgene