Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://antibodyregistry.org/AB_572263
Proper Citation: (ImmunoStar Cat# 20080, RRID:AB_572263)
Target Antigen: Serotonin
Host Organism: rabbit
Clonality: polyclonal
Comments: Manufacturer Applications: Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot; Note, The 5-HT Rabbit Antibody was raised against serotonin coupled to BSA with paraformaldehyde. The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/20,000-1/40,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA. Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.; Gene Symbol: Spl
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for 5-HT (Serotonin) Rabbit Antibody.
No alerts have been found for 5-HT (Serotonin) Rabbit Antibody.
Source: Antibody Registry
dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.