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Proteinase K from Tritirachium album Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

Cell permeable fungal toxin that disrupts contractile microfilaments by inhibiting actin polymerization. This, in turn, induces DNA fragmentation, inhibits cell division, and disrupts many cell processes. Inhibits glucose transport. One of a group of fungal metabolites that interfere with a wide variety of cellular movements. Useful tool for characterizing some of the polymerization properties of actin, and in studies on cytokinesis. Probe for the two hexose-transport systems in rat L6 myoblasts. PubChem Substance ID 24892890

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

Pepstatin A is an inhibitor of acid proteases (aspartyl peptidases). It forms a 1:1 complex with proteases such as pepsin, renin, cathepsin D, bovine chymosin, and protease B (Aspergillus niger). The inhibitor is highly selective and does not inhibit thiol proteases, neutral proteases, or serine proteases. Solubilized γ-secretase and retroviral protease are also inhibited by Pepstatin A. It has been used to characterize proteases from several sources. PubChem Substance ID 24898620

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

Inhibits the lysosomal proteinase cathepsin B, and the soluble Ca2+-activated proteinase. Many young plant tissues express a chomostatin-sensitive serine protease. Chymostatin is a strong inhibitor of many proteases, including chymotrypsin, papain, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases such as cathepsins A,B,C, B, H, and L. It weakly inhibits human leucocyte elastase. It is effective at a final concentration of 100 to 200 μg/ml (10 to 100 μM). Chymostatin is often included in protease inhibitor cocktails used with plant extracts. PubChem Substance ID 329775144

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

Induction of ovulation in mares and female fish. Induction and synchronization of ovulation in sows and gilts. Optimization of fertility in cows and heifers (repeat breeders). Treat

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format. Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

Porcine brain tubulin (>99% pure, see Cat. # T240) has been modified to contain covalently linked HiLyte Fluor™ 488 (HiLyte Fluor is a trademark of Anaspec Inc, CA) at random surface lysines. An activated ester of HiLyte Fluor™ 488 was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficient when protein bound is 76,000M-1cm-1). Final labeling stoichiometry is 1-2 dyes per tubulin heterodimer. HiLyte Fluor™ 488 labeled tubulin can be detected using a filter set of 440-460 nm excitation and 500-520 emission. HiLyte Fluor™ 488 tubulin is in a versatile, stable and easily shipped format. It is ready for micro-injection or in vitro polymerization. Cytoskeleton, Inc. also offers AMCA (Cat. # TL440M), rhodamine (Cat. # TL590M), X-rhodamine (Cat. # TL620M) and HiLyte Fluor™ 647 (Cat. # TL670M) labeled tubulins of the same quality.

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

Induction and synchronization of ovulation in cow, heifers, sheep & goat. Superovulation (required for embryo transplantation) in cow. Increase of fertility rate.

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

Thermo Scientific Leupeptin Protease Inhibitor reversibly inhibits serine and cysteine proteases. Catalog number: 78435

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

PubChem Substance ID 24277964

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• RRID-Legacy
• 6 years ago - submitted by Liliana Pineros Leyton

legacy version of open field locomotor activity software Fully Integrated System for up to 32 animals; Windows xp/vista/7 compatible Software; Powerful Windows based tools for collecting, storing, ... VersaMax System;This product has been replaced by Fusion Open Field - Locomotor Activity. INSUFFICIENT INFORMATION LETTER sent

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• RRID-Legacy
• 6 years ago - by Anonymous

Core provides molecular biotechnology services and current molecular genetic methods. Services include DNA and RNA Extraction, transgenic mouse genotyping, Human Cell line Authentication, Sanger DNA Sequencing, Next-Generation sequencing platforms, Microsatellite DNA Typing, Agena multiplex SNP Genotyping, Real-time PCR, NanoString Gene Expression Analysis. Specializes in custom workflows that couple services with specific research needs.

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• RRID-Legacy
• 6 years ago - submitted by Nate Herzog

GTAC offers comprehensive next generation sequencing, microarray, PCR and Bioinformatic services. In addition to generating high quality genomic, transcriptomic, and proteomic data, performs data analysis and provides technological support to users. Full service facility, from hypothesis to publication. Offers advanced analysis of microarray data. Provides free initial consultation to discuss project and offers several tiers of analysis packages to best suit your needs. NGS equipment includes NovaSeqs, Seqwell II, Oxford. Microarray expression and genotyping - all platforms.

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• 6 years ago - submitted by Nate Herzog

The oldest technical university in Poland, located in Warsaw, with faculties including architecture, machine design, electrical engineering, automotive and construction machinery engineering, electronics , information technology, mechatronics , geoinformatics, automatic control and robots, and biomedical engineering.

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• RRID-Legacy
• 6 years ago - submitted by Edyta Vieth

Software tool as code for processing and analyzing data in Allen Brain Atlas. Source code for reading and processing Allen Brain Atlas data. Allen SDK focuses on Allen Brain Observatory, Cell Types Database, and Mouse Brain Connectivity Atlas.

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• RRID-Legacy
• 6 years ago - submitted by Anita Bandrowski

Center to improve metadata and its use throughout biomedical sciences. Develops information technologies that make authoring complete metadata more manageable. Through better interfaces, terminology, metadata practices, and analytics. Optimizes metadata pathway from provider to end user. Provides way for funders to specify what metadata they want to collect as part of research life cycle.

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• dkNET
• 6 years ago - submitted by Ko-Wei Lin

For automated high throughput screening, cell proliferation assays and cytotoxicity assays, determining the number of viable cells in culture based on the amount of ATP present

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• RRID-Legacy
• 6 years ago - submitted by Entai Hou

rabbit anti-Caspase-3 (1:2000; Abcam Cat# ab184787) used for western blot

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• SciCrunch
• 6 years ago - submitted by Wenkai Chang

Cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside GM1, making it a powerful tool for retrograde labeling of neurons.

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• 6 years ago - submitted by Marin Manuel

Galaxy CLIP-Explorer can process large CLIP-Seq data of eCLIP, iCLIP, and with simple changes to the iCLIP workflows also FLASH, and uvCLAP. Software tools to process and visualise RNA protein interactions.

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• SciCrunch
• 6 years ago - submitted by Florian Heyl