Curator Dashboard

Facility is equipped with high performance computers, bioinformatics related software, biological databases, and data storage. Provides bioinformatics and computational services in virology, molecular biology, genomics, proteomics, next generation high throughput sequencing, and other biological related fields. For special requirements, can design and develop new algorithms, and implement programs and customize data processing pipelines. Core promotes education in bioinformatics to students and researchers, develops and delivers training material, including workshops that facilitate practical application of bioinformatics knowledge by students, postdoctoral fellows, and faculties in NCV community.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides support to University researchers for transmission- and scanning-electron microscopy. Based on center capacity, support is also available to researchers from other not-for-profit institutions.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provide members of University and their visitors with wide spectrum of optical microscopy equipment and extensive training in its use.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides help with grant review, information on data management plans,suggestion best practices for bionformatics analyses, advise on experimental design for Next Gen Sequencing (NGS) projects.Services include:Genome assembly and annotation,Transcriptome assembly and annotation,SNP/InDel calling,RNA-Seq analysis,ChiP-seq,Introgression mapping,novel gene discovery,Personalized GBrowse instances for data visualization,Access to high performance computing, Custom big data projects.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides tools of modern functional proteomics and metabolomics. Facility is equipped with chromatography and mass spectrometry based technologies for proteomics and metabolomics (Clinical and non clinical), personalized experimental design consultation and comprehensive, individualized bioinformatics support.Services include:Small molecule exact mass determination or quantitation using positive or negative ion mode;Protein identification using LC/MS/MS analysis and MASCOT and SEQUEST database search;Shot gun proteome analysis of biological samples;Biomarker discovery from biological fluid;Drug protein or drug nucleic acid protein interaction;Protein complex isolation and identifying interacting partners and its quantitation;Protein differential expression analysis and quantitation by 2D-LC MS/MS (MudPIT);Global PTM analysis and quantitation;Specialization in phosphorylation and oxidation analysis;Coomassie Blue and Silver Stained Gel analysis; de novo peptide sequencing by tandem mass spectrometry;Confirmation of mutations in protein ;Customized sequence search of in-house proteins that are not available in database; Post translational modifications (phosphorylation, sumoylation, ubiquitination, oxidation, etc.); Determination of oxidation state of cysteine (disulfide bonds);Intact proteins and peptides mass determination.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides flow cytometry instrumentation and expertise. Provides operator assisted analyzer and sorter use, as well as training and support for user instrument operation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides high resolution, light and electron microscopy technologies for imaging and analyzing molecular and structural organization of cells, tissue and bioengineered materials. Operates two sites at Stanford University: SOM Beckman Center CSIF and SOE Shriram Center CSIF.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides University of Massachusetts Medical School investigators with clinical research support in biostatistics, experimental design, and data management.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides system for Splicing isoform Annotation. This LISA platform allows high throughput annotation and functional analysis of Alternate Splicing in humans.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core sequencing services include Illumina Sequencers, Single-Cell RNA-Seq,Single-Cell Immune Profiling,Single-Cell ATAC-Seq,Linked-Read Sequencing. Provides bioinformatic analysis including Single-Cell RNA Analysis,Linked-Read Genome Analysis,ChIP-Seq Analysis Package,RNA-Seq Analysis Package,miRNA Analysis Package,Custom Project Requests,A La Carte Bioinformatics. Provides services including QX100 Droplet Digital PCR Machine (Biorad),Nucleic acid extractions from bio-specimens, Sample QC,Realtime PCR.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility operates as full service facility delivering transgenics and cell cultures to researchers and self service facility providing high quality space for researchers to use for their own specific project needs. Services available include transformations and cell/ tissue culture in many different plant species, project consultation, training workshops, self-service hood usage and offering high quality growth space. Facility staff has developed portfolio of transformation and tissue culture systems such as: Setaria, tobacco, petunia, tomato, Arabidopsis, soybean, Indian mustard, maize, rice and various different cell cultures. Within the facility, researchers have also produced cassava, sweet potato, and potato. Transformation systems will be added, developed, inherited, or modified as needed.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides analysis of gene expression using Affymetrix GeneChip platform. Offers next generation sequencing via Illumina NextSeq and Ion Torrent PGM and Proton instruments, analysis of RNA and DNA using Agilent Bioanalyzer.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core services include Custom DNA Sequencing (3730xl for dideoxy sequencing chemistry, or Illumina HiSeq 2500 for large scale sequencing projects),DNA Fragment Analysis,Sequencing and PCR troubleshooting and training,SNP analysis,Primer walking, Contig assembly and editing.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers protein/peptide sequencing, large and small scale peptide synthesis (Fmoc), matrix assisted laser desorption/ionization (MALDI) mass spectrometry, SDS-PAGE/electroblotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel and solution digestion, tandem mass spectrometry (LC-MS/MS), ion mobility mass spectrometry (IM-MS), digital image acquisition and analysis using Typhoon imaging system and 2D gel documentation/analysis system, and semi-preparative, analytical and micro-analytical high performance liquid chromatography (HPLC). MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, 2D gel documentation/analysis and Typhoon imaging system are all available for user operation after appropriate training.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Central micro/nano fabrication facility of Northwestern University. Provides range of nanofabrication equipment and technical expertise. Supports research in all areas of science, engineering, medicine, and interdisciplinary fields.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core research facility providing services in high-throughput DNA sequencing that includes staff experience in the construction of libraries for targeted amplicons, miRNA analysis, transcriptome profiling (RNA-Seq), whole exome sequencing (WES), epigenomic and genomic DNA analysis. Services in single cell library preparation are available using the 10x Genomics Chromium X system. In addition, spatial transcriptomics services using Visium HD arrays are provided. The GTC maintain and operate Illumina sequencing platforms; NovaSeq X Plus and MiSeq i100 instruments.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides expertise and equipment to conduct high throughput research. Services and equipment include:Next Generation Sequencing Services,Ion Torrent Proton sequencing,High Throughput Nucleic Acid Manipulation,Qiagen BioSprint 96 high-throughput DNA/RNA extraction system,Eppendorf AF2200 Plate Reader (absorbance and fluorescence based quantification),Lyophilization and Tissue Grinding,PCR Instruments,Two MJ Research 384 well 4 block DNA engine tetrads,Two Applied Biosystems 384 well 2 block Gene Amp PCR,Roche Lightcyler 480 RealTime thermocycler,Miscellaneous Services and Instruments,Blue Pippin and E-gel size selection systems,Agilent Bioanalyzer,Eppendorf epMotion M5073 liquid handling system.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core services include DNA sequencing, site directed mutagenesis, genome engineering (TALENs & CRISPRS), plasmid preparations and distributing clones/vectors from in house collections. Supports real time quantitative PCR through instrument training, troubleshooting and experimental design. Stowers researchers also have access to Illumina Next Generation Sequencing technology. Constructs libraries, performs sequencing and assists with high throughput genome sequencing, RNA-seq and ChIP-seq projects. Utilizes liquid handling and colony manipulation robots to automate many of services. Provides automation expertise and collaborate with researchers on custom automation projects.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers high field NMR instrumentation for structural studies of biological macromolecules. Instrumentation includes four-channel Bruker Avance 500, 600, and 700 MHz NMR spectrometers, ultra high sensitivity 5mm 1H-13C-15N triple-resonance cold probe for 600 MHz spectrometers. Service include acquisition and analysis of required spectra for elucidation of small molecule structures (includes synthetic molecules, natural products, cofactors, lipids, and short peptides (30 amino acids or less)). Types of projects conducted collaboratively include determination of three-dimensional structures of biological macromolecules, including proteins and nucleic acids, both alone and as complexes with various ligands.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to instruments, ranging from simple white light wide field microscopes to fluorescent multidimensional super resolution and confocal imaging systems. Provides expertise in areas of microscopy including laser scanning confocal microscopy, super resolution microscopy, two photon microscopy. Helps with image analysis software packages, including, Imaris, Volocity, Autoquant, MetaMorph, and NIS-Elements.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth