Curator Dashboard

Core provides seven mass spectrometry platforms, for shotgun and targeted analyses, run by core staff. Services include protein and peptide identification from gels and solutions, identification and localization of post translational modifications, relative and absolute quantitation of peptides and proteins, intact mass analysis of proteins in solution.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides researchers with ability to visualize samples, from monolayers and small organisms, such as developing fly and fish embryos, to very thick sections from brain and other organ tissues by using instruments including laser point (LSCM) and field scanning confocal (CSU), Total Internal Reflectance Fluorescence (TIRF), Multi Photon (MP), and Widefield (WF). Services in imaging in mammalian cells, yeast cells, Drosophila and Zebrafish embryos and ovaries, bacteria, sections of brain and other tissues, in both fixed and live specimens, Quantitative imaging methods such as FRAP, FLIP, and FRET, software packages for image processing, analysis, and 3D image reconstruction.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core facility that supports expression, purification, and analysis of reagent and preclinical proteins by providing instrumentation and consultation for protein production from small to large scale. Available equipment includes shaking incubators, 5 L fermentor, high pressure cell disruptor, hollow fiber concentrator, Maxwell16 magnetic bead purification system, AKTA Purifier chromatograph, analytical ultracentrifuge, analytical HPLC, and MALDI-QIT-TOF mass spectrometer.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides two experimental 7 Tesla magnetic resonance imaging machines which were purchased by Center for Stroke Research CSB (Device 1) and Cluster of Excellence NeuroCure (Device 2) with aim of creating experimental platform for small animal imaging. Services projects in areas of experimental neuroimaging and molecular imaging, but projects in other fields of research are explicitly encouraged.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides complete CRISPR services, design, production of gRNAs & Cas9 mRNA, and injection.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides instrumentation in area of solution phase, high resolution NMR spectroscopy and expertise. Primary solution state spectroscopy of bio molecules including, but not limited to, studies of polymers, minerals, soils, and extracts. Equipment includes: 800MHz Varian Inova Spectrometer, 600MHz Varian Inova Spectrometer, 500MHz Varian Inova Spectrometer, and staff assistance/consultation is available.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides flow cytometry services to scientists, offers analytical cytometry and high-speed sorting either in investigator operated format or facility staff assisted format, offers training for research personnel interested in learning how to design flow cytometry experiments, operate instrumentation and analyze data. Services include experimental design, protocol development, sample staining, instrument operation, data analysis, graphical production, sample processing as a Service (SpaaS). Facility is equipped with three cell sorters and four cell analyzers.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides mass spectrometric services, from basic mass measurement to complex proteome analyses.Services include basic mass measurement, ESI-MS/MS,LC-MS,Exact mass measurement,Protein identification.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core using ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) offers high-throughput analytical services for determining diagnostic amino acids and their precursors or metabolic products. Available biomarkers include citruilline and tetrahydrobiopterin. Determination of other small molecules is possible.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Services include advise on microscope to use for research project, image acquisition, optimization image analysis and sample preparation, free of charge access to 500GB per laboratory on CMCI server in order to store data for project. Facility houses four wide-field, near-field (TIRF), three confocal (swept-field and laser-scanning) microscopes and laser capture microdissection system.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides services in sequencing using ABI model 3730 DNA Sequencer with the XL Upgrade,design and have synthesized primers for primer walking experiments, DNA fragment analysis such as microsatellites, AFLP, SNP analysis.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides bioinformatics research resources, services and training in support of existing research programs and in development of new programs and grant applications. Provides next-generation sequencing data analysis including RNA-Seq and QuantSeq analysis, de novo transcriptome assembly, whole-genome sequencing, genome resequencing, single-cell sequencing, metagenomics, microbiome, mass spectrometry proteomic analysis of label-free (DIA) and TMT data, analysis of metabolomics, microbiome, multi-omics data, data mining, modeling, and visualization. Provides customized bioinformatics and statistical services including data quality control, power studies, hypothesis tests, regression, clustering, network analysis, function annotation, pathway analysis including iPathwayGuide.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core mass spec and proteomic services include open access lab for trained users with GC/MS, LC/MS, high resolution LC/MS, and MALDI-TOF instruments, help with intact protein analysis, targeted quantitation, drug discovery support, pathway analysis, protein interactions, FFPE tissue analysis, both labeled and label-free proteomics, and more. Please contact SUMS to discuss these and other custom projects including new application development.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core assists with epigenetic analysis including DNA Methylation, Chromatin Immunoprecipitation and Real Time Quantitative PCR gene expression analysis. Services include DNA Methylation Analysis, Specific Genomic Location Analysis:Methylation Specific PCR,Bisulfite Sequencing,Bisulfite Pyrosequencing,Qiagen PyroMark Pyrosequencer Instrumentation;Genome Wide Analysis:High Throughput Sequencing Methylation Analysis, Methyl-Sensitive Cut Counting (MSCC),Methyl CpG Binding Domain - Isolated Genome Sequencing (MiGS);Chromatin Immunoprecipitation Analysis (ChIP):Analysis of Histone Modifications,DNA-Protein Interactions,Chromatin Positions analyzed using:Quantitative PCR Analysis (Real-Time QPCR),High Throughput Sequencing Analysis (ChIP-Seq);Gene Expression Analysis (QPCR):Real-Time Quantitative PCR Gene Expression.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility within UW Biotechnology Center (UWBC) for assisting researchers with their data analysis needs. Helps with variety of common sequencing related projects, DNA-seq, ChIP-Seq, RNA-Seq, 16S metagenomics, and small RNA analyses.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides imaging systems including light/fluorescence microscopes, confocal laser scanning microscopes and electron microscopes.Provides services, teaching, training and technical support for multi disciplinary research.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides analysis of proteins, peptides, lipids, polar metabolites and small molecules by high resolution/high mass spectrometry. Targeted and hypothesis free analysis are offered combined with relative quantitation based on either label free, tandem mass tags or metabolic labelling. Acquisition tools include Data Dependent (DDA) and Data Independent (DIA).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides consulting and services in X-ray Photoelectron Spectroscopy (XPS or ESCA), Time-of-Flight Secondary-Ion Mass Spectrometry (TOF-SIMS), X-ray Photoelectron Imaging, Auger Electron Spectroscopy (AES), Scanning Auger Microscopy or Imaging (SAM), Secondary-Electron Microscopy (SEM), Ion-Scattering Spectroscopy (ISS or LEIS), Scanning Probe Microscopy (STM and AFM).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core produces fully human, full length, antigen specific antibodies for use in studying human immune responses. Produces high affinity protective antibodies to influenza, anthrax lethal toxin, and various S. pneumonia polysaccharides. Helps quantify antibody secreting cell responses after vaccination and by generating human monoclonal antibodies to be characterized. Pathogen specific human monoclonal antibodies are also available for licensing agreements and other forms of commercial development. Services include to define temporal human immune responses after vaccination and to generate human monoclonal antibodies to supplement research,development of novel methods to produce human monoclonal antibodies after acute infection and other immune events, investigation of new technologies for characterization of human monoclonal antibodies produced by core.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides fully automated high throughput screening (HTS) of Compound Libraries (130,000+ compounds) for both enzyme/protein-based assays and cell-based assays, using Caliper Life Sciences Staccato system;Genomic siRNA screening with siARRAY whole human genome siRNA library from Dharmacon targeting 21,000 genes, using Agilent Bravo system;High-Content Screening using ImageXpress Micro automated fluorescent microscope with live cell, bright field, phase contrast and integrated plate handling with Thermo Catalyst CRS, and image analysis using MetaXpress software;High Throughput Molecular Biology reagents and services, including access to cDNA libraries (Human ORFeome collection, 15,000 genes) and 96 and 384-well bead clean-ups and PCR setup (Biomek FX and Agilent Bravo), and other automation steps in collaboration with SFGF;High-throughput assay development assistance with cell culture, experiment design, robotic programming and Standard Operating Procedure drafting;Screening data analysis assistance with protocols, hit determination and structure activity analyses using MDL chemical database ISIS/HOST, Plate Manager, Assay Explorer and Report Manager. Use of microplate reader detection systems, including Tecan Infinite M1000 and Infinite M1000 PRO and Molecular Devices Analyst GT for fluorescence; fluorescence polarization; time-resolved fluorescence; absorbance and luminescence (with injectors and AlphaScreen); and Flexstation II 384, for kinetic fluorescence reads to measure calcium mobilization and ion channels.Use of liquid-handling robots, including Sciclone ALH3000 (96- and 384-well pipetting), Agilent Bravo (96- and 384-well pipetting), Velocity11 VPrep (96-well pipetting), Bio-Tek plate washers/dispensers, Matrix Wellmate and Titertek/Labsystems Multidrop microplate dispensers, and Velocity11 PlateLoc plate heat sealer;Training for most of these services.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth