Curator Dashboard

Core specializes in cell, protein, and small molecules analysis as well as cell culture techniques. Services include:2-D gel electrophoresis, 2-D DIGE, LC-MS/MS, HPLC, flow cytometry, fluorescence-activated cell sorting (FACS), cell and tissue culture, and immortalization of cell lines. Our staff works closely with investigators to help design, perform, and analyze experiments.Offers training and assistance in flow cytometry, tissue culture, and operation many of our walk-up instruments.Instruments:Cell Sorter: FACS Aria III, BD Biosciences;Flow Cytometers, analyzers:C6, Accuri/BD Biosciences;Novocyte 3000, ACEA Biosciences;software for analysis: FSC Express, DeNovo software;LC-MS/MS: 6460 Triple Quadrupole, Agilent;Typhoon Trio Scanner, GE Lifesciences;Blood Analyzer: Hemavet 950, Drew Scientific.Plate Readers:;Victor Nivo 5F, Perkin Elmer;Luminometer: Centro XS, Berthold.Services:Cell Sorting (FACS);2-D gel electrophoresis/2D-DIGE;LC-MS/MS analysis of compounds; Cell immortilization.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core mission is to ensure quality and regulatory compliance of Memorial Sloan Kettering Cancer Center�s clinical trials investigational products and Investigational New Drug (IND) applications, and to promote efficiency in our drug development projects, through combination of advisory, project management, and quality assurance (QA) oversight services.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Microscopy core specializing in laser based fluorescence techniques. Offers training and expertise in 20 different microscope systems, including live cell and in vivo imaging, laser scanning (LSM) and spinning disk (SDC) confocal, multi-photon (2p), fluorescent lifetime imaging (FLIM), light-sheet microscopy (SPIM), super resolution (Airyscan), slide scanning and patterned illumination for optogenetic manipulation and readout. Provides offline computer analysis workstations for image processing, visualization and analysis, including GPU workstations. MIC operates in 3 different buildings on campus, with primary locations in Life Sciences Addition (LSA), North-side core in Barker Hall, and small outpost in Li Ka Shing Center for Biomedical and Health Sciences (LKS).Provides equipment in categories:Confocal and multi photon laser scanning microscopes,Spinning disk confocal microscopes,Lightsheet (SPIM) microscopes,Epifluorescence/widefield scopes and Computer workstations.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core located on campus of UC Davis, providing fully customized support for scientific research using genetically altered mice. Provides access to develop, plan, execute, and analyze research project involving genetically-altered mice. Provides description of project, including alternative approaches, accurate timelines, and cost estimates.Provides regular and informative communication throughout project, including email updates on completion of each milestone. Project progress can be followed at each stage by viewing detailed information anytime by accessing our online and secure project tracking system (PTS) available 24 hours a day.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core supports cytogenetic analysis of cells from human and research animal sources. Range of chromosome analysis techniques are ranging from basic G-banded karyotyping, to 24-color Spectral Karyotyping (SKY), as well as Fluorescence in situ Hybridization (FISH) mapping of cloned DNAs.Services include:Karyotype characterization of new cell lines,Confirmation of cell line identity or stability,Karyotype screening of ES cells prior to transgenesis,Mapping of transgene integration sites,Chromosome or locus copy number enumeration,Publication-quality illustrations.Cytogenetic techniques available:Short-term cell culture,Metaphase chromosome preparation,G-banded karyotyping.FISH techniques available:DNA labeling for custom probe generation (for FISH projects),DNA amplification by DOP-PCR,Chromosome painting,FISH mapping (on chromosomes, interphase nuclei or DNA fibers),Tissue FISH (paraffin or frozen sections),Immuno-FISH (DNA FISH combined with fluorescent immunostaining) , Spectral karyotyping (SKY),Q-FISH (measurement of telomere lengths).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core facility for high throughput services covering the areas of Capillary Sequencing, Mouse Genotyping, SNP Genotyping, Clinical Diagnostic Sequencing, Cell Line Identification, Gene Expression Analysis and DNA/RNA extraction.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides researchers with equipment and expertise required for multi-dimensional imaging of cells and tissues at high resolution and to perform quantitative image analysis. Resources include widefield, confocal, multi-photon, TIRF, super-resolution and electron microscopy, as well as equipment for live cell imaging and image analysis. Services include sample preparation, imaging, molecular localization, and image analysis. Provides training, technical support, sample preparation, imaging assistance, collaboration and consultation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core used to develop custom monoclonal antibodies and obtain large scale quantities of existing ones for experiments. Core provides cell line distribution service and tests samples for mycoplasma contamination.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core Facility was closed in November 2016. Services of shRNA Core were redistibuted to other existing facilities at Einstein.Gene Modulation Services: CRISPR, RNAi and ORF. CRISPR-Cas9 services for cell lines will now be performed in Gene Modification Facility. Gene Modification Facility already offers CRISPR services for genetic modification of mice and will utilize this scientific expertise to provide CRISPR services for the gene modification of cell lines as well. You can access these services through core's site in iLab.The human and mouse whole genome shRNA library has been relocated to the Molecular Cytogenetic Core. The core staff will pull requested shRNA and ORF clones from our collection for investigators. You can access this service through the core's site in iLab.Access and operation of the Operetta instrument for high-content imaging will now be coordinated through the Macromolecular Therapeutics Development Facility (MTDF). You can access this service through the core's site in iLab. Lentivirus prep from shRNA and CRSPR constructs can be obtained from our Gene Therapy Core from clones obtained from the Molecular Cytogenetic Core. You can access this service through the core's site in iLab.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to electron microscopy equipment and services which includes AFS 2 Freeze Substitution robot,Denton DV502 Carbon Evaporator,JEOL JEM 1010,JEOL JEM 1400plus,JEOL JEM 3200FS,Polaron E5100 sputter coater,Vitrobot Mk3,Wohlwend High Pressure Freezer. Users have to be included on user e-mail lists and have iLab account in order to use core.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core assists in developing and performing high-throughput assays involving NCEs, siRNA, shRNA and dsRNA.Core also houses High Content image-based platform for chemical and biology research using both live and fixed cells. Services include High Throughput Screening,Assay development and validation (Cell-based/ Biochemical),siRNA screens,Small molecules screens,Proof of concept with validation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core specializes in production of pure, functional proteins for structural, biophysical, and biochemical studies. Facility offers three categories of service:Protein Expression,Protein Purification,Scientific Consultation, Mentoring, and Training; Offers Isotope labeled proteins for NMR;High production scales for immunizations, drug discovery, structural biology;Endotoxin-free protein production;Stable cell line generation;Expert baculovirus expression;Custom packages to efficiently suit your needs.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides expertise in assay development towards high-throughput applications and high content screening services, provides technologies for cell-based screening such as robotic liquid handling stations, plate readers and several automated microscopes. Services include developing complex automated image analysis pipelines and multi-parametric analysis of high content imaging applications.Equipment:Flexible liquid handling work stations Biomek FXp (Beckman), Freedom Evo 200 (TECAN);Multilabel microtiter plate readers Envision (Perkin Elmer), GENios Pro (TECAN);Acoustic dispensing (Labcyte Echo 550);CellVoyager CV7000 automated live cell confocal microscope (Yokogaw);Perkin Elmer OPERA;Operetta widefield microscope (Perkin Elmer);Automated confocal microscopy OPERA (Perkin Elmer);Washers, dispensers;96-well electroporation system (amaxa nucleofector).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core to support academic and industrial users. Spectrometer time is available to trained users for an hourly fee. Lab manager trains new users, consults to determine whether NMR will be useful in their research, and helps design experimental plan to obtain information they need. Manager also operates spectrometer for users whose experiments do not justify time and expense of individual training.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core performs and analyzes integrated clinical genomic, molecular, microarray, FISH, and cytogenetic analyses to diagnose broad range of inherited diseases and cancer. Serves as centralized clinical testing laboratory for Children Oncology Group leukemia, Wilms tumor, medulloblastoma, and rhabdomyosarcoma studies. Emphasizes collaborative interactions between clinicians, physician-scientists, and basic science investigators to quickly transition cutting edge research results into cutting edge diagnostics, using technology platforms. Services include Whole Exome Sequencing (WES),cytogenetic chromosome analysis,Fluorescence in situ Hybridization,Chromosomal microarray analysis,Molecular Genetic Testing - Inherited Diseases,Molecular Genetic Testing - Cancer.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides support and infrastructure necessary to initiate and successfully complete structural biology or structural chemistry project. Offers services in Crystallization,X-Ray DiffractionData Collection,Structure Determination and Refinement.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides services which include massively parallel sequencing on Illumina MiSeq, traditional Sanger sequencing, fragment analysis, quantitative PCR, nucleic acid extractions and QC, and digital PCR. Offers various level of service for Sanger sequencing and qPCR from full service to ready to load plates. Trains researchers in laboratory techniques and provide protocols. Each MiSeq sequencing project and qPCR project is unique and specifically designed so experimental design should be discussed before collecting samples.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers services for: High quality synthetic peptides, stable isotope labeled peptides, peptides with PTM and fluorescent and affinity tags, synthesis of peptide libraries. Analysis of synthetic peptides. Purification, lyophilization and aliquoting of synthetic peptides.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides consultations and collaborations on research studies requiring computational structural biology methods. Analyses available are to study of static structures, molecular dynamics studies for analyzing contribution of dynamic and collective motions to macromolecular functionality. Trains researchers in computational structural biology techniques, or works in collaborative manner with researchers.Provides access to software tools for protein and DNA sequence analysis, protein fold determination, homology modeling, active site identification, and analysis of effects of various mutations on structure and function of protein, along with additional computational analyses.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides equipment and resources for crystallographic analysis of target macromolecules and small inorganic or organic molecules. Performes crystallization, X-ray diffraction data collection (both in-house and synchrotron sources) processing, phasing, crystallographic refinement, model building, and visualization. Structural data provide information including biological functions, 3D-folding, ligand binding (small molecule or protein), or mutational effect of target macromolecules. Services include Crystallization Screening; Data Collection Service: Diffraction experiments will be performed at in-house X-ray facility as well as synchrotron beam lines; Structure Determination and Analysis: complete data set collected at home or synchrotron will be processed for inorganic, organic, protein or nucleic acid structure determination, model building and crystallographic refinement. Other Structural Biology Related Services: core facility offers thorough analysis of macromolecular structure for publication or grant applications, also provides computational experiments such as homology modeling and in silico ligand docking.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth