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Core provides equipment and technical expertise for evaluation of molecular and morphological changes in cells, tissues, and organs following exposure to complex environmental contaminants. Provides equipment, including automated tissue processor, paraffin embedding center, two automated microtomes, cryostat, vibratome for soft-tissue sectioning, multiheaded light microscope with projection capabilities, system for laser capture microdissection, and slide scanner with analysis software for identification and quantification of morphological structures.Offers expertise in histopathological and immunocytochemial methods, including fixation, dehydration, embedding, sectioning, histological staining, immunolabeling, high-resolution imaging, and quantitative image analysis. Services in sample preparation, offers assistance in imaging and image analysis and provides consultation for ongoing or future research projects, training program for students and investigators who use centrally available equipment.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Open access core facility available to all TAMU users for Metabolomics service and research. Provides different levels of service from drop-off to collaborative projects.Training for TAMU postdoctoral fellows and students to use instrumentation in support of specific projects.Short courses and workshops on advances in metabolomics. Instrumentation for targeted and untargeted metabolomics analysis.Provides mass spectrometers that are coupled to liquid chromatography (LC) or gas chromatography (GC) separation systems.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core to facilitate, amplify, and accelerate biological research and discovery through application of bioinformatics. Provides bioinformatic analysis services for Next Generation Sequencing (NGS) data.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Nanowell Cytometry platform is shared resource housed in Flow Cytometry Core that provides Koch Institute and MIT researchers access to Microengraving and SeqWell technologies, developed in Love and Shalek Laboratories at Koch Institute. Microengraving platform enables measurement of multiple traits from individual cells derived from wide array of tissues or cell types, such as immune cells either in their native condition or upon stimulation. Platform allows simultaneous assessment from small or large numbers of cells. Additionally, it is possible to obtain genetic information from individual cells of interest. Platform has been employed in projects related to antibody discovery, tumor profiling from various cancer tissues, and immune cell functions in various allergic, infectious or auto-immune diseases.Core offers access to CellCelector, tool which enables users to isolate single cells using ultra-precise robotic micromanipulator. Offers access to nanowell microplates which enable automated isolation of monoclonal and viable clones. Seq-Well is portable, low-cost platform for massively-parallel single-cell RNA-Seq. Barcoded mRNA capture beads and single cells are sealed in array of subnanoliter wells using semi-permeable membrane, enabling efficient cell lysis and transcript capture.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides central hub to help leverage bioinformatics and data science methods in research projects. Services include Transcriptomics: bulk RNA-Seq, single-cell RNA-Seq, and spatial RNA-Seq; Genomics: SNP, structural variants (SV), and copy number variation (CNV) analyses; Regulatory genomics: ChIP-Seq, ATAC-Seq, CUT&RUN, and Hi-C; Functional genomics: CRISPR-based loss-of-function screens;Image and metadata analysis: AI/ML-based approaches including knowledge graphs. Offers training, education, collaboration, consultation,custom guidance on study design and analysis approaches. Provides classroom and custom training sessions on computational, data science, bioinformatics topics, tailored workshops for requested specialized topics. Offers technical infrastructure and support, computing resource optimization and facilitation, bioinformatics software deployment (conda environments and Docker containers), version control and reproducible science (Git repositories), data management support and submission to public repositories.

• Resource
• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility offers histological services including Tissue Sectioning:Paraffin embedding and frozen embedding in OCT;Paraffin section serial, random, serial interrupted;Frozen section thaw mount cold/warm, serial, free floating,thick slice, random and serial interrupted, CryoJane tape transfer;Vibratome section;Routine staining, i.e., H+E, Nuclear Fast Red Special staining, Alcian Blue, Cresyl Violet, PAS, Oil Red , Toluidine Blue, Von Kossa, Combined Massons Elastin, Picrosirius Red, Sudan IV, Thionin, Prussian Blue, Movats Pentachrome, Masson Trichrome, Alcian Blue/Phloxine/Tartrazine, others available upon request. Immunohistochemistry/Enzyme:ABC method (direct/indirect);Fluorescence;Biocytin detection;HRP;Single and multiple-labeling;Edu/Brdu,in situ Hybridization,RNAScope,BaseScope.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides technical expertise, training and access to instrumentation, enabling and supporting use of flow cytometry techniques. Allows simultaneous multiparametric analysis of many thousands of cells per second, enabling trained researchers to rapidly analyze complex cell populations using benchtop analysis flow cytometers. High-speed assisted cell sorting services provide researchers with fast, objective and quantitative recording of fluorescent signals from individual cells combined with physical separation of cells of particular interest.Services include High-end, staff assisted cell sorting, using four different high speed sorters;Unassisted bench-top analysis available 24/7;Assisted bench-top analysis available by appointment;Extensive training on bench-top analyzers;Extensive training on FACS Aria sorters;Consultation on experimental design and data interpretation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core Facility provides access to Atomic Force Microscope and Leica SP5 Scanning Laser Confocal Microscope, located in Room 122 of Pickus Center for Biomedical Research on Biddeford Campus.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides computational resources and expertise to enhance productivity of researchers studying infectious diseases. Assists with virtual screening, protein-small molecule docking, binding site prediction, protein modeling and design, prediction of protein stability changes upon mutation, fragment based probe design, as well as preparation of presentation graphics.Specializes in initial hit identification of non-traditional drug targets such as protein-protein or protein-RNA interfaces by offering high-throughput virtual screening via pocket optimization with exemplar screening at protein-protein interfaces and hotspot pharmacophore mimicry of protein-RNA interactions.CCB works in collaboration with Molecular Graphics and Modeling Laboratory.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides means to grow thin films of metals and metal oxides. Facility contains two state of art deposition chambers PVD PLD/MBE 2300 and a PVD nanoPLD 1000. PLD provides convenient way to make thin films of materials heretofore studied in bulk by pressing and sintering target from bulk material; facilities are available at Northwestern for pressing and sintering of targets.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to advanced chromatographic and mass spectrometry instrumentation. Main analytical focus is quantitative analysis of small molecules (drugs, drug metabololites, endogenous metabolites, xenobiotics and toxicants) and intact large molecules (biologics, intact proteins, and biopolymers like hyaluronan). Provides analytical instruments including Liquid Chromatography and Mass Spectrometry, Microbalances, Quantitative PCR (qPCR or Real-Time PCR), Sample Preparation Aids, Pharmacology Department Shared Instruments.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core at University of Washington (UW) is managed by Department of Comparative Medicine and open to investigators with research needs to maintain mice free of any microorganisms (germ-free), or with known population of microorganisms (gnotobiotic).Services provided include Rederivation, Germ-Free (GF) Mice, Isolator Maintenance of GF Mice, Experimental Study Support, Diet Sterility Testing, Technical Procedures.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides technical assistance to Brown researchers by performing flow cytometry-based analysis and sorting. Facility has 4-laser, 19-parameter BD FACSAria IIIu for flow and sorting applications, as well as 3-laser, 15-parameter BD FACSCelesta equipped with high throughput system for analysis of samples from 96 well plates.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to expertise, training and specialized instrumentation related to tissue processing, sectioning, staining, immunohistochemistry and microscopy. Offers services and training related to image analysis and image analysis software to guide investigators in choosing best methods for presenting their data.Services include Trimming of wet tissues,Tissue processing into paraffin, OCT and paraffin embedding, Sectioning of paraffin-embedded/frozen tissues, Routine and special histochemical staining, Immunohistochemistry/Immunofluorescence, Antibody optimization, Brightfield/ widefield/ confocal microscopy, Image capture and image analysis.Core has cryostats, microtomes and microscopes available for reservation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides technical expertise, training, instrumentation and related services for assessing behavior in animals to help to gain insight into function of nervous system and mechanisms of acute and chronic pain. Services include Behavioral Testing; General Behavioral Phenotyping: Observational Screens, Motor Function; Pain/Sensory Testing: Thermal, Mechanical, Chemical; Additional Neurobehavioral Tests: Psychiatric, Addiction, Learning and Memory;Miscellaneous Systems: GI Transit, Cardiovascular and Respiration. Other Services: Assistance choosing behavioral test and statistical tests for assessing results; Training in surgical techniques for small animal surgeries; Staff expertise includes surgical methods for producing pain models; dosing of drugs/anesthetics including central, systemic and localized administration; and tissue extractions.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides viral vector services dedicated to development and production of viral vectors for angiogenesis, stem cell and regenerative medicine and clinical renal research. Viral vectors are produced in dedicated BL2 facility within MMCRI building. Vector systems currently being used include adenovirus, retrovirus and lentivirus. Services Available:Transfection and transduction of gene-specific viral DNA provided by principal investigators;Small, large and custom scale production of adenovirus;retrovirus and lentivirus (2nd and 3rd generation packaging);TCID50 titration of adenovirus;Titration of retrovirus and lentivirus (fluorescence marker or antibiotic resistance);Test for replication competent virus (RCV) in lentivirus preparations.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core offers researchers, clinicians and biotech companies regulatory compliant labs and services to manufacture minimally manipulated and complex biotechnology products.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides expertise and resources for large scale biology. Helps to set up, run, gather data and perform analysis in drug discovery research, biochemistry, cell and organismal biology, functional genomic screening, and synthetic genetic. Works with proteins, nucleic acids, small model organisms, and microbial strains. Provides tissue culture,produces and uses lentivirus particles, screens compound libraries, does experiments for investigators,generates preliminary data to figure out if idea is workable, discusses project development. Services include Macromolecular binding, biochemical, and cell-based assays,High content screening with widefield or confocal optics,Nanoliter liquid handling up to 1536-well density,Whole-plate kinetic assays (ion currents, GPCR signaling),Compound library screening,CRISPR/Cas9 screening (multiplexed libraries),Analysis of large data sets,Fluorescence Thermal Shift assay (measures protein melting),Complex liquid handling work flows.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core is Northwestern Radiology research facility providing translational imaging capabilities that promote pre-clinical and clinical research efforts. CTI occupies space in basement of Olson building housing imaging equipment along with research staff. Services include Cardiovascular Imaging for development, analysis and application of MRI methods providing insights into structure and function of cardiovascular system,NeuroImaging for functional MRI using spectroscopy and diffusion-weighted imaging to studying human anatomy and physiology during development and disease,Small Animal Imaging for molecular and functional imaging of biological processes in living animal models to study diseases and responses to intervention.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides mechanical testing machines and accessories for conducting educational, research, and outreach experiments on most solid materials.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth