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reagnet used to isolate RNA

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• 6 years ago - submitted by Kaira Church

solution used for antigen retrieval for immunohistochemistry

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• 6 years ago - submitted by Kaira Church

master mix for qPCR

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• 6 years ago - submitted by Kaira Church

Web server as semi automated tool that identifies nucleotide sequences in scientific publications, determines whether they are correctly identified. Seeks for nucleotide sequences in pdf files and then call local version of blastn. Text mining and text analysis tool to allow peers to identify errors in published or forthcoming scientific literature. Used to search and extract gene identifiers and nucleotide sequences against human genomic and transcript database.

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• 6 years ago - submitted by Tracey Weissgerber

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris. The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 µg). This means that users can process both small and large sample sizes with the same RNA isolation kit.

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• 6 years ago - submitted by Lucia Garcia del Valle

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.

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• 6 years ago - submitted by Lucia Garcia del Valle

AllStars Negative Control siRNA is the most thoroughly tested and validated negative control siRNA currently available. This siRNA has no homology to any known mammalian gene. Validation has been performed using Affymetrix GeneChip arrays and a variety of cell-based assays to ensure minimal nonspecific effects on gene expression and phenotype. Minimal nonspecific effects ensure that comparison of the gene-specific siRNA to the negative control gives a true picture of the effects of target-gene knockdown on gene expression and phenotype. If the negative control causes nonspecific effects then results from RNAi experiments can be misleading and difficult to interpret. Cloning experiments confirmed that AllStars Negative Control siRNA enters RISC. AllStars Negative Control siRNA is patent-pending and the sequence is proprietary.

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• 6 years ago - submitted by Arindam Dey

Custom-made siRNAs Sense: 5’-GAA UAA AUG CAG UAU CUA AAU-3’; Antisense:5’-UUA GAU ACU GCA UUU AUU CGG-3’

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• RRID-Legacy
• 6 years ago - submitted by Arindam Dey

AllStars Negative Control siRNA is the most thoroughly tested and validated negative control siRNA currently available. This siRNA has no homology to any known mammalian gene. Validation has been performed using Affymetrix GeneChip arrays and a variety of cell-based assays to ensure minimal nonspecific effects on gene expression and phenotype. Minimal nonspecific effects ensure that comparison of the gene-specific siRNA to the negative control gives a true picture of the effects of target-gene knockdown on gene expression and phenotype. If the negative control causes nonspecific effects then results from RNAi experiments can be misleading and difficult to interpret. Cloning experiments confirmed that AllStars Negative Control siRNA enters RISC. AllStars Negative Control siRNA is patent-pending and the sequence is proprietary.

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• RRID-Legacy
• 6 years ago - submitted by Arindam Dey

NESSICARE - Non woven swabs – Sterile – 10x10 cm - CNST-300D Sterile, single use. The non-woven compresses of NESSICARE consist of viscose (70%) and polyester (30%). Sterilized with Ethylene oxide. Nessicare non-woven compresses have the following benefits: - Optimal absorption - Does not fluff - Does not lengthen - Are pleasing to the touch (softness) - Are available in different sizes Composition: 70% viscose, 30% polyester, latex free. Sterilization: Ethylene oxide

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• 6 years ago - submitted by Arindam Dey

This 1X Red Blood Cell (RBC) Lysis Buffer is formulated for optimal lysis of erythrocytes in suspensions of a single type of cells derived from hematopoietic tissues of mice, such as the spleen, as well as in suspensions of human peripheral blood. This tampon contains ammonium chloride, which lyses red blood cells without having a major effect on lymphocytes when the instructions are followed correctly. Ammonium chloride does not effectively lyse nucleated red blood cells.

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• 6 years ago - submitted by Arindam Dey

Falcon® 100mm x 15mm Standard Style Bacteriological Petri Dish, not treated polystyrene, 20/pack, 500/case, sterile Durable construction for stable dish manipulation Crystal-grade, virgin polystyrene for optical clarity

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• 6 years ago - submitted by Arindam Dey

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a fluorometric method for estimating the number of nonviable cells present in multiwell plates. The assay rapidly measures the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into a fluorescent resorufin product. The amount of fluorescence produced is proportional to the number of lysed cells using a 96- or 384-well format. The CytoTox-ONE™ Reagent does not damage normal healthy cells; therefore the reactions set up to measure released LDH can be performed directly in a homogeneous format in assay wells containing a mixed population of viable and damaged cells.

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• 6 years ago - submitted by Arindam Dey

Accutase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is the "gentler form" of trypsin. It can replace Trypsin/EDTA for the detachment and dissociation of anchorage-dependent cells from surfaces. It can also be used on suspension cells to reduce clumping in preparation for counting.

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• 6 years ago - submitted by Arindam Dey

This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria.

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• 6 years ago - submitted by Arindam Dey

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

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• 6 years ago - submitted by Arindam Dey

Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance.

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• 6 years ago - submitted by Arindam Dey

Gibco® 2-mercaptoethanol (also known as beta-mercaptoethanol or BME) is a powerful reducing agent used in cell culture media to prevent toxic levels of free radicals. Mercaptoethanol is not a stable solution. This is why most protocols require daily supplementation. Mercaptoethanol Gibco®-2 is available at a concentration of 50 mM in saline in Dulbecco phosphate buffer (DPBS).

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• 6 years ago - submitted by Arindam Dey

Sodium pyruvate is commonly added to cell culture media as a carbon source in addition to glucose. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement for all cell cultures. However, if cells have been grown in medium that is supplemented with sodium pyruvate, we recommend continuing use of the supplement as cell growth may lag without it. Gibco® Sodium Pyruvate (100 mM) is formulated using 11 g per liter of water. The final concentration of sodium pyruvate used in most cell culture media is 1 mM.

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• 6 years ago - submitted by Arindam Dey

The non-essential Gibco® MEM amino acids are used to supplement cell culture media, in order to increase cell growth and viability. Gibco® MEM non-essential amino acids contain the same non-essential amino acids as those found in the minimum essential medium (MEM) at 100X power.

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• 6 years ago - submitted by Arindam Dey