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Core provides training to use latest episomal techniques to reprogram, expand and characterize human and mice iPS cells from skin or blood tissues of healthy subjects and diseased patients. Develops capability to differentiate iPS cells into specific somatic cells, such as neutrons, cardiomyocytes, and hepatocytes.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides preclinical and clinical imaging instrumentation and techniques.Preclinical services include Bioluminescence,Fluorescence,In situ cryoimaging,Magnetic Resonance Imaging (MRI),Positron Emission Tomography (PET),Radiochemistry Synthesis, Scintigraphy,Ultrasound,X-ray / Computed Tomography (CT) / micro CT,Image Processing / Quantification clinical research imaging systems. Clinical services include Comprehensive MR imaging research services, Dedicated Siemens Skyra 3T MRI scanner, Large animal preclinical studies, or clinical human research may be conducted,Structural and functional brain scanning can be performed with Avotec LCD Projection System, Coodination of access to PET and CT scanners for additional preclinical and human imaging studies. Core includes PET radiopharmaceutical core facility. Core staff provide radiochemistry synthesis.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility serves as specialized laboratory performing correlative assays for cancer-based clinical trials. Participates in project development to assist in assay selection and optimization prior to study initiation. Offers pick-up service for specimens from patients at U of C Hospital or outpatient clinics, and process samples as indicated for assays being performed. For some studies, isolation and freezing of peripheral blood lymphocytes for analysis at later date is performed, while for others immediate staining of whole blood for specific cell surface markers of interest is carried out. Prepares clinical grade products, such as peptide vaccines, for administration into patients.Assays performed include ELISA, ELISPOT, tetramer binding assays, detection of cell surface markers by flow cytometry, and biochemical assays such as Western Blots, RNA extraction from tumor biopsies and either real time RT-PCR for specific transcripts or preparation of samples for gene expression profiling. Facility is equipped with MACSQuant Flow Cytometer, ELISA microplate reader, ELISPOT reader, PCR thermocyclers, CO2 incubators, biosafety cabinets, centrifuges, and equipment for Western blot analysis. Performs assays on human samples but consideration will be given to expanding these services to mouse-model systems.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides access to biophysics equipment, integral to research community at University of Chicago.Provides tools, training, and assistance for quantitative analysis of macromolecules and their interactions. Instruments include Seahorse XFe-96 Extracellular Flux Analyzer,Jasco J-1500 CD Spectrometer,Synergy Neo HST Plate Reader,Wyatt DAWN HELEOS II SLS,Wyatt DynaPro Plate Reader DLS,Wyatt DynaPro NanoStar DLS,HORIBA Fluorolog-3,Microcal iTC200,Molecular Imager,Bio-Rad ProteOn XPR to Biacore 8K,Agilent 8453 Spectrophotometer. Core provides staff assistance in conducting experiments.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility provides high throughput cell- and non-cell-based screening. Has number of validated cell lines and variety of small molecule or siRNA screening libraries available to investigators.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides training, assistance and services in light microscopy, electron microscopy and image analysis. UNC core facility that is part of Department of Pathology and Laboratory Medicine, and are light microscopy core for Lineberger Comprehensive Cancer Center.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides DNA sequencing services including DNA extraction, cell line identification, microsatellite analysis, and antibody sequencing,DNA Sequencing, Monoclonal Antibody Sequencing,Nucleic Acid Quantification,PCR Machine Hire,Real-Time PCR Robotic Liquid Handling,Taqman SNP Analysis.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core facility that conducts fee for service mass spectrometry-based non-targeted and targeted metabolomic studies. The facility serves and partners with both on- and off-campus clients.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides instrumentation and proteomics expertise to Brown University and Rhode Island-EPSCoR scientific communities and training in emerging proteomic techniques. Mass Spectrometry proteomics resources and services are provided by COBRE Center for Cancer Research Development (CCRD) at Rhode Island Hospital: Proteomics Core.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Facility maintains instrumentation, provides hands-on training to new users, experimental design expertise, assistance with data analysis and support for incorporating NMR into grants and manuscripts. NMR spectrometers are available at 800 MHz, 700 MHz, 500 MHz and 400 MHz (2), corresponding to magnetic field strengths ranging from 18.8 T to 9.4 T. Data processing and analysis software is available on workstations in NMR Facility. The facility supports scientific inquiry in research areas such as structural biology, organic chemistry, natural products analysis and environmental studies.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides services including Peptide Synthesis and Purification,Protein/Peptide Sequence Analysis,DNA Synthesis and Purification,Sanger DNA Sequencing,Next Generation Sequencing.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides services and advanced instrumentation support for nucleic acid (DNA and RNA) analysis, high-throughput next-generation sequencing, including solutions for sample processing such as nucleic-acid isolation, nucleic-acid quantitative and qualitative analysis, NGS library preparation, quantitative-PCR analysis, etc. Provides sample processing and library preparation such as whole genome sequencing, shotgun metagenomics, metatranscriptomics, targeted amplicon sequencing, RNA-Seq, ChIP-Seq, Exome Sequencing, Methyl-seq, Single Cell Genomics, etc. Facility accepts samples and will perform requested analysis. Offers training to users to conduct experimentation.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Provides immunology assays, expert consultation, and education to UCSF and global research community. CIL specializes in developing immunology research tools, and initiating and stimulating innovative research projects to address emerging questions in immunologic diseases including HIV pathogenesis.Services include:Isolation of PBMC for staining, cell sorting, or viable cryopreservation;Isolation of mucosal mononuclear cells (MMC) from mucosal tissue biopsies;Immunomagnetic bead isolation of untouched cell populations for functional studies;Immunophenotyping of whole blood, PBMC, MMC and cerebrospinal fluid for: NK, B and T cells percentages, and their subsets, including maturation, trafficking, senescence, activation, in vivo proliferation and chemokine receptor expression;Flow cytometric functional assays, including NK cell function, antigen-specific CD4+ and CD8+ T cell cytokine expression, degranulation and proliferation;Evaluation of T cell signaling by phosflow using monoclonal antibodies specific for phosphorylated protein;Training and Support;Flow Cytometry Training Course;Three-hour lecture and lab course designed to cover basics of flow cytometry including:Fluorescence emission and detection,Fluidics,Operation,Panel design,Controls.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Database that annotates SNPs with known and predicted regulatory elements in intergenic regions of H. sapiens genome. Known and predicted regulatory DNA elements include regions of DNAase hypersensitivity, binding sites of transcription factors, and promoter regions that have been biochemically characterized to regulation transcription. Source of these data include public datasets from GEO, ENCODE project, and published literature.

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• RRID-Legacy
• 7 years ago - by Anonymous

Open access database for all cognitive data collected from touchscreen related tasks. Performs data comparison and interactive data visualization for any data uploaded onto the site. There are also guidelines and video tutorials available.

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• NIF
• 7 years ago - submitted by Maryann Martone

Flow cytometry facility offering training and services including:Access to two, 17-color BD LSR II analytical instruments with High Throughput Sampler (HTS) module,Configurations:LSRII 1,LSRII 2;Help with Flow Cytometry Panel Design;Fluorofinder (access our cytometers under CFAR Immunology Core);BD Panel designer;SFGH LSRII Flow Core Protocols;LSRII Startup and Shutdown;How to run the CST calibration assay;Access to a 17-color BD FACSAria II for fluorescence-activated cell sorting (FACS);4-way tube sorting;96 well plate sorting;Index sorting;SFGH ARIA Flow Core Protocols and configuration;ARIA Startup;Determining Drop Delay;Side Stream Set Up;Clog Procedure;ARIA Shutdown Protocol;ARIA Configuration;DNA analysis with standard dyes;Analysis of CFP, GFP, YFP, mRFP, mTomato, and mCherry gene expression proteins;Calcium flux measurements using Indo-1;Training of users on the operation of instruments and experimental design through the CIL Flow Cytometry Course;Maintaining and Upgrading Instruments;Research Support Services (study design, assay selection, grant and paper writing support).

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides ES cell services with high probability of germline transmission. Offers ES cell targeting, genomic DNA extraction from 96-well plates, expansion of targeted ES cells, chromosome counts, and preparation of ES cells for microinjection.Prior to initiation of project, consultation is available on entire procedures of generating knockout mice. Core works with Gladstone Transgenic Gene Targeting Core for your microinjections to deliver full-range gene targeting service;CRISPR gRNA cloning,Cell-based functional test to identify best-performing TALENs or sgRNAs for your gene-editing experiment via mismatch-based assays such as Surveyor or T7E1;In vitro RNA synthesis - can help to make RNAs for your zygote injection or RNA transfection. We have TALEN and Cas9 plasmids with either T7 or T3 promoter subcloned in for efficient in vitro synthesis.sgRNAs for CRISPR can be synthesized off T7-sgRNA PCR product. Quality of synthesized RNAs will be checked via bioanalyzer;Custom TALEN to make double-strand breaks in genome;ES cell targeting (feeder-independent).Investigators targeting construct will be electroporated by core personnel. We have two feeder-independent ES cell lines, E14 (129-derived) and JM8A3.N1 (C57BL/6-derived) you can choose from. After drug selection for about one week, up to 300 colonies will be picked. When they are about to be confluent, we will split them as duplicate, one master plate to freeze for future expansion of positive clones and one plate for genotyping to identify targeted ES cell clones. Your plates for genotyping will be ready for pick-up 2-3 weeks after electroporation date.Genomic DNA extraction from ES cells on 96-well plate;Expansion of targeted clones from core targeting (up to 5 clones),A maximum of 5 positive clones will be thawed from 96-well plates and expanded to 6-wells. We will freeze 5 vials (each about 1 million)/clone for future use and give you 1 vial-equivalent cells to validate your genotyping before injection. It takes about 10 days to expand and freeze down cells;Expansion of ES cells from outside resources (per clone) Investigators provide one vial of frozen ES cells with information about culture condition from original resource. We will revive, nurture, and refreeze ES cells (5 vials) when they are ready. In addition, we will give you 1~2 million cells for your genotyping verification;Preparation for microinjection;Chromosome counting;Custom services.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core is equipped with two benchtop analyzers from BD Immunocytometry Systems (3 laser/8 color BD Canto II and 4 laser/17 color BD LSR II). These instruments support large variety of applications, including multicolor cell phenotyping, apoptosis, cell cycle and bead arrays. After orientation and training, equipment is available for use directly by investigators. Alternatively experiments can be run and analyzed by Core Facility staff.For sorting purposes facility is equipped with high speed BD FacsAria IIu sorter (4 laser/11 colors), as well as magnetic sorter (AutoMacs Pro) from Miltenyi Biotech. FacsAria IIu is enclosed in Baker Bioprotect III to support sort of biohazardous agents level 1 and 2.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides equipment used for structural modeling in crystals and in solution, using X-ray diffraction and scattering, as well as magnetic resonance.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth

Core provides service support to all MIT investigators who utilize specialized in vitro cells such as stem cells, organoids, or primary cell lines and/or novel mouse models to study human diseases such as cancer. Projects involve generation of new model system, such as CRISPR-mediated gene editing in mouse to introduce mutation that mimics one found in patients. Helps with projects required optimization of finicky cell cultures and other challenges.Provides customizable set of service options to match specific needs of each project, including consultative advice and troubleshooting, complete tissue culture and microinjection services within our facilities or hands-on training to enable investigators to perfom these experiments either at their own laboratory or within our facilities.Services Include:Gene Targeting genomic modification through traditional or CRISPR/Cas9 locus targeting, assistance with targeting strategies and vector designs;Embryonic Stem Cells generation of new ES lines from mouse strains, importation and testing of lines from outside sources, differentiation of ES lines into specific cell lineages or cell types and more;Microinjection injection of mouse ES cells into blastocysts to generate chimeras and injection of DNA, RNA or CRISPR RNPs into the pronucleus of fertilized mouse eggs to generate transgenic and edited mice;Specialized Tissue Culture establishemnt of new primary cell cultures from a tumor, tissue or organ; Isolation of fibroblasts (MEFs) from mice for culture and analysis;Tissue Culture for Xenograft and Syngenic Modeling optimization, validation and testing of cell lines for orthotopic placement into mice, coordinated with Preclinical Testing Facility;Repository of Reagent Mice Commonly used wild type mice such as C57BL/6j as well as KrasG12D-based models of cancers are maintained on campus for efficient distrubution;Training and Troubleshooting for all aspects of embryonic stem cells, primary cultures, animal breeding etc.;Serum, DMEM, LIF and other media components that have been tested and verified for use with ES cells.

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• SciCrunch
• 7 years ago - submitted by Edyta Vieth