Curator Dashboard

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. The calculated molecular weight of Recombinant Human IGF-II is 7.5 kDa.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Ki-Sun Park

This assay employs an antibody specific for mouse IGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Ki-Sun Park

PD98059 is supplied as a lyophilized powder. For a 20 mM stock, reconstitute 1.5 mg in 280 μl anhydrous DMSO (5 mg in 933.3 μl anhydrous DMSO). For 50 mM stock, reconstitute 1.5 mg in 112 μl anhydrous DMSO (5 mg in 373.3 μl). Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 5 - 50 μM for one hour prior to treating with a stimulator.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Ki-Sun Park

BMS-754807 is an orally bioavailable antagonist of human insulin-like growth factor type I receptor (IGF-1R) with potential antineoplastic activity. BMS-7548077 binds to IGF-1R, preventing IGF-1 ligand binding and activation of IGF-1R-mediated signaling pathways; inhibition of IGF-1R-mediated signaling pathways may result in the inhibition of tumor cell proliferation and the induction of tumor cell apoptosis. IGF-1R is a receptor tyrosine kinase expressed on most tumor cells and is involved in mitogenesis, angiogenesis, and tumor cell survival

• Resource
• RRID-Legacy
• 5 years ago - submitted by Ki-Sun Park

RNAscope® 2.5 HD Detection Reagents-RED assay are designed for RNA in situ hybridization (ISH) based on ACD's patented signal amplification and background suppression technology. This manual assay is a single-plex, chromogenic-RED assay. The detection reagents contains AMP 1, APM2, AMP 3, AMP 4, AMP 5, AMP 6, Fast RED-A, Fast RED-B. The detection reagents provide enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in).To run the detection reagents you will need the pretreatment kit and the wash buffer reagents.Disclaimer: Photos 2.5 products will be updated shortly.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Ki-Sun Park

RNAscope® 2.5 HD Assay- BROWN,RNAscope® 2.5 HD Assay- RED,RNAscope® 2.5 HD Duplex Assay,RNAscope® Multiplex Fluorescent Assay

• Resource
• RRID-Legacy
• 5 years ago - submitted by Ki-Sun Park

Software Python tool as Vanderbilt University curation tool.Used for exporting public profiles of university faculty from Vanderbilt University database into Wikidata.Used to create or update items in Wikidata using data from CSV spreadsheet data.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Lane Rasberry

Non-biting midge

• Resource
• RRID-Legacy
• 5 years ago - submitted by Makoto Ishimota

Brefeldin solution

• Resource
• RRID-Legacy
• 5 years ago - submitted by Francesco Marangoni

Recombinant Mouse CD86-Fc Chimera

• Resource
• RRID-Legacy
• 5 years ago - submitted by Francesco Marangoni

mouse CD80-Fc Chimera fusion protein

• Resource
• RRID-Legacy
• 5 years ago - submitted by Francesco Marangoni

WB, ELISA, Flow, ICC/IF, IHC, IHC-P

• Resource
• RRID-Legacy
• 5 years ago - submitted by 志达 单

Open source whole genome association analysis toolset, designed to perform range of basic, large scale analyses in computationally efficient manner. Used for analysis of genotype/phenotype data. Through integration with gPLINK and Haploview, there is some support for subsequent visualization, annotation and storage of results. PLINK 1.9 is improved and second generation of the software.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous

pCMV6-AN-mRFP, mammalian vector with N-terminal mRFP

• Resource
• SciCrunch
• 5 years ago - submitted by Kienan Savage

pCMV6-AN-Myc-DDK, mammalian vector with N-terminal Myc-DDK tag

• Resource
• SciCrunch
• 5 years ago - submitted by Kienan Savage

Dual Selection Vector Gateway entry vector

• Resource
• SciCrunch
• 5 years ago - submitted by Kienan Savage

DAPI Fluoromount-G® is a water-soluble, 4',6-diamidino-2-phenylindole (DAPI) containing compound recommended for slides mounted after a staining procedure having an aqueous final step. When bound to DNA, DAPI fluoresces and is typically used as a nuclear/chromosomal counterstain. Since DAPI Fluoromount-G® is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened. This mounting medium also provides a semi-permanent seal for storage of slide preparations.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Paul Mathews

Immunogen: Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary. Isotype: IgG. Clonality: Polyclonal. Host : Rabbit. Gene: NFIX. Purity: Immunogen affinity purified.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Qiangqiang Zhang

Knowledge-as-a-Service for the RI development communities to document RI services' development and operation and address engineering problems. More specifically, the knowledge management system should (1) ingest technical results from ENVRIplus, FAIR assessment (FAIR data are data that meet principles of findability, accessibility, interoperability, and reusability), the key sub-domains, and other tasks using a formal language for knowledge representation and proven semantic technologies; (2) provide services and tools to enable RI developers and data managers to browse, search, retrieve and compare RI technical statuses and technical solutions to development problems via available content; (3) provide content management tools for specialists in the ENVRI community to ingest new knowledge and control the quality of content; (4) also provide interfaces to other existing semantic resources, e.g., the service catalog of a future ENVRI-HUB, to enhance knowledge discovery and cross-RI search, between knowledge services and the online presence of ENVRI resources.

• Resource
• RRID-Legacy
• 5 years ago - submitted by Siamak Farshidi

Software tool for protein coding gene prediction for prokaryotic genomes.

• Resource
• RRID-Legacy
• 5 years ago - by Anonymous