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Species: Other
Genetic Insert: 6xbsMS2-random
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27009120
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148431 Copy
Species: Other
Genetic Insert: K0-Rluc
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: reporter for Ribosome Quality control Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148846 Copy
Species: Other
Genetic Insert: BFP
Vector Backbone Description: Backbone Size:2218; Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33499763
Proper citation: RRID:Addgene_149352 Copy
Species: Other
Genetic Insert: UnaG
Vector Backbone Description: Backbone Size:2218; Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33499763
Proper citation: RRID:Addgene_149354 Copy
Species: Other
Genetic Insert: mem-TurboID
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_149410 Copy
Species: Other
Genetic Insert: ER-TurboID
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199915
Proper citation: RRID:Addgene_149412 Copy
Species: Other
Genetic Insert: mem-TurboID
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199915
Proper citation: RRID:Addgene_149413 Copy
Species: Other
Genetic Insert: Cyto-TurboID
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199915
Proper citation: RRID:Addgene_149414 Copy
Species: Other
Genetic Insert: ER-TurboID
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199915
Proper citation: RRID:Addgene_149415 Copy
Species: Other
Genetic Insert: BFP
Vector Backbone Description: Backbone Size:3894; Vector Backbone:pUC19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33499763
Proper citation: RRID:Addgene_149344 Copy
Species: Other
Genetic Insert: mcherry
Vector Backbone Description: Backbone Size:2218; Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33499763
Proper citation: RRID:Addgene_149345 Copy
Species: Other
Genetic Insert: Gateway cassette (ccdb + Chlor.R.)
Vector Backbone Description: Vector Backbone:pMK33-Act5c; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:33443210
Proper citation: RRID:Addgene_149610 Copy
Species: Other
Genetic Insert: FlaviA-mNeptune
Vector Backbone Description: Backbone Marker:Eric Campeau, Paul Kaufman; Backbone Size:7871; Vector Backbone:pLenti CMV GFP Puro (658-5) (Addgene Plasmid #17448); Vector Types:Mammalian Expression, Lentiviral, High expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The FlaviA-mNeptune reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter and/or generate OSER structures - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-CMV-FlaviA-mNeptune-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon flavivirus infection.
Proper citation: RRID:Addgene_140091 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage MS2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of mutant MS2CP (MS2CP(V29I)) was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140260 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pGbait; Vector Types:Mouse Targeting; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140279 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pBluescript II SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140273 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pX330A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140285 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pX330A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140280 Copy
Species: Other
Genetic Insert: APEX2-NLS
Vector Backbone Description: Vector Backbone:pEF1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140281 Copy
Species: Other
Genetic Insert: T7-lacO-QacR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140389 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
