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Species: Escherichia coli
Genetic Insert: e. coli galactokinase
Vector Backbone Description: Backbone Size:10000; Vector Backbone:PMSG346; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20851171
Proper citation: RRID:Addgene_26958 Copy
Species: synthetic gene
Genetic Insert: P#38-luxAB
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27061 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27060 Copy
Species: synthetic gene
Genetic Insert: P#38-mcherry rfp
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27058 Copy
Species: synthetic gene
Genetic Insert: P#38-gfp-DAS+4
Vector Backbone Description: Backbone Size:6087; Vector Backbone:pTE-0X1-GCT6; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Comments: pTE-mcs derivative (Addgene plasmid# 20320) with GFP-DAS+4 under the control of a strong promoter. DAS+4 sequence is AANDENYSENYADAS at end of GFP.
Proper citation: RRID:Addgene_27057 Copy
Species: synthetic gene
Genetic Insert: P#38-luxA-FLAG-luxB-DAS+4
Vector Backbone Description: Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21075796
Proper citation: RRID:Addgene_27064 Copy
Vector Backbone Description: Backbone Marker:Glickman Lab; Backbone Size:4152; Vector Backbone:pmsg360; Vector Types:mycobacterial ko vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20472794
Comments: Note that Addgene's sequencing results differ from the depositor's sequence at bp# 561, 565 and 829. Depositing scientist states that these differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_27155 Copy
Genetic Insert: tetR(sB), clpXtb-K127R
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:6181; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Hygromycin
Defining Citation: PMID:19174563
Proper citation: RRID:Addgene_27374 Copy
Species: Synthetic
Genetic Insert: crRNA to ligD
Vector Backbone Description: Vector Backbone:pMN016; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: Shuttle vector Mycobacterium smegmatis/E.coli
Proper citation: RRID:Addgene_104623 Copy
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104907 Copy
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104912 Copy
Vector Backbone Description: Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:22885758
Comments: Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117497 Copy
Vector Backbone Description: Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30319771
Comments: This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli.
This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
References: this paper has been accepted in RSC Advances:
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam
Proper citation: RRID:Addgene_117695 Copy
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30698703
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117828 Copy
Species: A. niger
Genetic Insert: pTEF1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118076 Copy
Vector Backbone Description: Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018.
Proper citation: RRID:Addgene_118229 Copy
Species: Synthetic
Genetic Insert: Fab_Quin-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119049 Copy
Species: Synthetic
Genetic Insert: Fab_MTX-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119047 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119885 Copy
Vector Backbone Description: Backbone Size:5835; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126598 Copy
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