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Species: Homo sapiens
Genetic Insert: FANCD2
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_001018115,NM_001319984,ENST00000383807,ENST00000419585 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_144382 Copy
Vector Backbone Description: Backbone Marker:Addgene #155184; Backbone Size:5575; Vector Backbone:XLone-Puro Cas13d-eGFP U6 BbsI; Vector Types:Mammalian Expression, Piggybac transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35567999
Comments: A plasmid that expresses Cas13d is needed. To clone Cas13d gRNA for gene knockdown, digest the plasmid with BbsI and then ligate annealed oligo into the backbone. gRNA could be designed using https://cas13design.nygenome.org/ and annealing oligoes should have the following format, where Ns are the gRNA sequence and Ms are reversed sequences:
FWD:5'- AAACNNNNNNNNNNNNN
RVS: 5'-AAAAMMMMMMMMMMM
Proper citation: RRID:Addgene_159756 Copy
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Destination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216077 Copy
Species: Other
Genetic Insert: CD81 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Positive Controls; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216091 Copy
Species: Synthetic
Genetic Insert: GFP and luciferase
Vector Backbone Description: Vector Backbone:PLOV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33810026
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.17.431647v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_161030 Copy
Species: N. furzeri
Genetic Insert: DDX5-IDR(483-634)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38810650
Comments: Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
Proper citation: RRID:Addgene_166166 Copy
Species: Other
Genetic Insert: CD97, CD4, CD26, CD274 guides
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Positive Controls; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216095 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9 [Sp]
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Assembled Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: lentiCRISPRv2 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216097 Copy
Species: Enhanced Acidaminococcus sp.
Genetic Insert: Cas12a [EnAs]
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Assembled Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: pRDA_550 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216096 Copy
Species: N. furzeri
Genetic Insert: DDX39A
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38810654
Comments: Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482120 for bioRxiv preprint. Also see Harel and Chen et al. 2024 (https://doi.org/10.1016/j.celrep.2023.112787).
Proper citation: RRID:Addgene_166161 Copy
Species: N. furzeri
Genetic Insert: DDX1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38810654
Comments: Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482120 for bioRxiv preprint. Also see Harel and Chen et al. 2024 (https://doi.org/10.1016/j.celrep.2023.112787).
Proper citation: RRID:Addgene_166160 Copy
Species: N. furzeri
Genetic Insert: DDX5ΔIDR(1-483)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38810650
Comments: Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
Proper citation: RRID:Addgene_166165 Copy
Species: N. furzeri
Genetic Insert: DDX5ΔPrD(1-535)
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38810650
Comments: Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
Proper citation: RRID:Addgene_166164 Copy
Species: N. furzeri
Genetic Insert: DDX3X
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38810654
Comments: Stopless entry clone. Please visit https://doi.org/10.1101/2022.02.26.482120 for bioRxiv preprint. Also see Harel and Chen et al. 2024 (https://doi.org/10.1016/j.celrep.2023.112787).
Proper citation: RRID:Addgene_166162 Copy
Species: Other
Genetic Insert: CD59 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Positive Controls; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216087 Copy
Species: Other
Genetic Insert: CD47 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Positive Controls; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216089 Copy
Species: Synthetic
Genetic Insert: EGFP-KASH
Vector Backbone Description: Backbone Marker:NIDA GEVVC; Vector Backbone:pOTTC1553; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32234214
Proper citation: RRID:Addgene_131684 Copy
Species: Synthetic
Genetic Insert: EGFP-KASH
Vector Backbone Description: Backbone Marker:NIDA GEVVC; Vector Backbone:pOTTC407; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32234214
Proper citation: RRID:Addgene_131682 Copy
Species: Enhanced Acidaminococcus sp.
Genetic Insert: Cas12a [EnAs]
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Assembled Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: pRDA_174 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216099 Copy
Species: Other
Genetic Insert: CueR
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37699423
Proper citation: RRID:Addgene_167216 Copy
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