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Species: Homo sapiens
Genetic Insert: SFXN4
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:5600; Vector Backbone:pMXs-IRES-Blasticidin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30442778
Proper citation: RRID:Addgene_110638 Copy
Species: Homo sapiens
Genetic Insert: SFXN2
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:5600; Vector Backbone:pMXs-IRES-Blasticidin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30442778
Proper citation: RRID:Addgene_110636 Copy
Species: Homo sapiens
Genetic Insert: SFXN5
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:5600; Vector Backbone:pMXs-IRES-Blasticidin; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30442778
Proper citation: RRID:Addgene_110639 Copy
Species: Synthetic
Genetic Insert: Lac promoter with lac operator and RBS
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110582 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29608282
Proper citation: RRID:Addgene_110621 Copy
Species: Homo sapiens
Genetic Insert: St1Cas9 LMD-9
Vector Backbone Description: Vector Backbone:pX602 (Plasmid #61593); Vector Types:Mammalian Expression, Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31900288
Comments: Mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9 was subcloned from MSP1594 (Plasmid #65775). An SV40 NLS was added to the N-terminus. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint
Proper citation: RRID:Addgene_110624 Copy
Species: Homo sapiens
Genetic Insert: St1Cas9 LMD-9
Vector Backbone Description: Vector Backbone:MSP1594 (Plasmid #65775); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31900288
Comments: This plasmid is identical to MSP1594 (Plasmid #65775) except for the addition of an SV40 NLS to the N-terminus of mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint
Proper citation: RRID:Addgene_110625 Copy
Species: Moniliophthora roreri
Genetic Insert: S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5
Vector Backbone Description: Vector Backbone:pCHINT2A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29651464
Comments: Insert sizes: 1947, 1569, 1248, 1953, 1881
Proper citation: RRID:Addgene_110691 Copy
Species: Moniliophthora roreri
Genetic Insert: PKS
Vector Backbone Description: Vector Backbone:pCHINT2A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29651464
Proper citation: RRID:Addgene_110690 Copy
Genetic Insert: B. fragilis 16S toehold switch sensor
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30131493
Proper citation: RRID:Addgene_110696 Copy
Genetic Insert: CPSF6-eGFP
Vector Backbone Description: Backbone Marker:Tyler Jacks, Addgene plasmid #11578; Backbone Size:6852; Vector Backbone:pSICO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29643241
Proper citation: RRID:Addgene_110693 Copy
Species: Homo sapiens
Genetic Insert: BRAFV600E IRES H2B-mOrange2
Vector Backbone Description: Vector Backbone:pHIV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110732 Copy
Genetic Insert: B. thetaiotaomicron 16S toehold switch sensor
Vector Backbone Description: Vector Backbone:pCOLA; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30131493
Proper citation: RRID:Addgene_110697 Copy
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pPNT6; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Targeting vector with Neo flanked by 2 LoxP sequences. Also called pPNT3LoxP2. Ligation of Neo Cassette from pPNT3.LoxP5' cut AscI/XbaI into pPNT3.LoxP3' cut AscI/XbaI. The resulting plasmid has Neomycin Cassette flanked with 2 LoxP sequences oriented in the same direction.
Proper citation: RRID:Addgene_11072 Copy
Species: Homo sapiens
Genetic Insert: p14ARF IRES H2B-mOrange2
Vector Backbone Description: Vector Backbone:pHIV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Comments: Addgene sequencing detected a potentially mixed population, with a C to T nucleotide change that would cause a T8I mutation within the CDKN2A insert. Please screen multiple clones to ensure that the correct (C) one is isolated before proceeding with downstream work.
Proper citation: RRID:Addgene_110731 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A gRNA
Vector Backbone Description: Vector Backbone:pEN35; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110736 Copy
Species: Homo sapiens
Genetic Insert: CDKN2A gRNA
Vector Backbone Description: Vector Backbone:pEN35; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110737 Copy
Species: Homo sapiens
Genetic Insert: H2B-mOrange2
Vector Backbone Description: Vector Backbone:pHDR; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29990501
Proper citation: RRID:Addgene_110735 Copy
Vector Backbone Description: Backbone Size:5450; Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin
Defining Citation: PMID:17117155
Proper citation: RRID:Addgene_110739 Copy
Species: Talaromyces cellulolyticus
Genetic Insert: PKS
Vector Backbone Description: Vector Backbone:pCHINT2A; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29651464
Proper citation: RRID:Addgene_110680 Copy
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