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Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 R775A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111943 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 Q776A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111944 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 R744A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111948 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 R186A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111939 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 R181A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111937 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 R182A
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111938 Copy
Genetic Insert: None
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112040 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERG5
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112041 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ORM1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112045 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FET3
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112042 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NSG2
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112043 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 310-972
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5208; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111994 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PST1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Comments: Please note that Addgene NGS results identified an ambiguous base (A/C) at position 2762, in yEGFP. Ura marker present. This could result in a G67C mutation in yEGFP, though this is not expected to affect plasmid function.
Proper citation: RRID:Addgene_112046 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 237-972
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5208; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111992 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsh155 472-972
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:5208; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29752352
Proper citation: RRID:Addgene_111996 Copy
Genetic Insert: NEDD4 WW2 domain fused to C-terminal split mCherry, neg ctrl interacting peptide fused to N-terminal split mCherry
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pNAS1b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889213
Comments: Backbone originally described in Sawyer, et al, Designed Phosphoprotein Recognition in Escherichia coli, 2014.
Binding domain under araBAD, phosphosite/peptide cassette under pLtetO
Proper citation: RRID:Addgene_112030 Copy
Species: Arabidopsis thaliana
Genetic Insert: IAA17
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112034 Copy
Genetic Insert: 14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pNAS1b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889213
Comments: Backbone originally described in Sawyer, et al, Designed Phosphoprotein Recognition in Escherichia coli, 2014.
Binding domain under araBAD, phosphosite/peptide cassette under pLtetO
Proper citation: RRID:Addgene_112031 Copy
Genetic Insert: Gal DNA binding-estradiol binding-MSN2 activator
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112037 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Su9
Vector Backbone Description: Vector Backbone:pRS313; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112038 Copy
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