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Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pRibo BsaHind; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the periplasm of mycobacteria. It is episomal and multi-copy. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111699 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111732 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111737 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Please screen multiple clones and validate plasmid by sequencing or restriction analysis before proceeding with downstream experiments
Proper citation: RRID:Addgene_111738 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111736 Copy
Species: Homo sapiens
Genetic Insert: BLM
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9388193
Proper citation: RRID:Addgene_111722 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Please screen multiple clones and validate plasmid by sequencing or restriction analysis before proceeding with downstream experiments
Proper citation: RRID:Addgene_111725 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111729 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111792 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111790 Copy
Species: Mus musculus
Genetic Insert: mSesn2
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25040165
Proper citation: RRID:Addgene_111796 Copy
Species: Mus musculus
Genetic Insert: mSesn2
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25040165
Proper citation: RRID:Addgene_111797 Copy
Species: Mus musculus
Genetic Insert: mSesn2
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25040165
Proper citation: RRID:Addgene_111798 Copy
Species: Synthetic
Genetic Insert: D44.1
Vector Backbone Description: Backbone Size:6168; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31442220
Comments: The amino acid sequence is taken from the deposited structure 1MLC in the protein data bank.
Proper citation: RRID:Addgene_111718 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting eGFP 171-191
Vector Backbone Description: Backbone Marker:Lynne Postovit (Addgene plasmid # 74293); Backbone Size:10694; Vector Backbone:All_in_one_CRISPR/Cas9_LacZ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29989627
Proper citation: RRID:Addgene_111821 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting eGFP 294-314
Vector Backbone Description: Backbone Marker:Lynne Postovit (Addgene plasmid # 74293); Backbone Size:10694; Vector Backbone:All_in_one_CRISPR/Cas9_LacZ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29989627
Proper citation: RRID:Addgene_111822 Copy
Species: Mus musculus
Genetic Insert: Hic-5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15687259
Proper citation: RRID:Addgene_11190 Copy
Species: Danio rerio
Genetic Insert: ZFERV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14694121
Comments: ZFERV PCR insert (the single 3' A overhangs on both ends are not included): 5'-TCTAAAGGAAAATGAACTTAACAGTTGCGAG (31 nt total upstream of the ZFERV sequence), CGAGgacaac agtggaatgg aatctttgtt aaatttagca
aagtacagta aactgaacag agtattgcga ata -3'
(73 nt total downstream of the ZFERV sequence).
A map of pCRII-TOPO is available at Addgene's vector DB.
Proper citation: RRID:Addgene_11192 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting RAF1 94-114
Vector Backbone Description: Backbone Marker:Lynne Postovit (Addgene plasmid # 74293); Backbone Size:10694; Vector Backbone:All_in_one_CRISPR/Cas9_LacZ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29989627
Proper citation: RRID:Addgene_111826 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting eGFP 349-369
Vector Backbone Description: Backbone Marker:Lynne Postovit (Addgene plasmid # 74293); Backbone Size:10694; Vector Backbone:All_in_one_CRISPR/Cas9_LacZ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29989627
Proper citation: RRID:Addgene_111823 Copy
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