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Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111783 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the cytoplasm of mycobacteria. It is a single-copy integrating vector in mycobacteria. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111700 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111788 Copy
Species: Other
Genetic Insert: APEX2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for IPTG-inducible expression of APEX2 in the E. coli cytoplasm. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111702 Copy
Vector Backbone Description: Backbone Size:3200; Vector Backbone:pGEMT-easy; Vector Types:cloning intermediate; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28526819
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111773 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111777 Copy
Species: Mus musculus
Genetic Insert: Mef2c, Tbx5 and Gata4
Vector Backbone Description: Backbone Size:5871; Vector Backbone:pMx; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25416133
Proper citation: RRID:Addgene_111810 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111778 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pGEMT-easy; Vector Types:cloning intermediate; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28526819
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_111812 Copy
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Marker:Cellbiolabs; Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28526819
Comments: Please note that there are some discrepancies between Addgene's quality control sequence and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
NheI right after iRFP670 was destroyed during cloning, containing half SpeI and half NheI.
Proper citation: RRID:Addgene_111816 Copy
Species: Homo sapiens
Genetic Insert: GGA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11177 Copy
Species: Synthetic
Genetic Insert: Clover-GGSGGS9-mRuby2 fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5402; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30024378
Comments: Partially based on Addgene Plasmids #49089 and #21769
Proper citation: RRID:Addgene_111762 Copy
Species: Synthetic
Genetic Insert: Clover-GGSGGS5-mRuby2 fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5402; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30024378
Comments: Partially based on Addgene Plasmids #49089 and #21769
Proper citation: RRID:Addgene_111760 Copy
Species: Homo sapiens
Genetic Insert: RBAP2
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4969; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11174 Copy
Species: Homo sapiens
Genetic Insert: BLM
Vector Backbone Description: Backbone Marker:Invitrogen (thermoFisher); Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11971187
Proper citation: RRID:Addgene_111766 Copy
Species: Gallus gallus
Genetic Insert: Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5399; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30024378
Comments: Partially based on Addgene Plasmids #26019, #49089, and #21769
Proper citation: RRID:Addgene_111764 Copy
Species: Homo sapiens
Genetic Insert: PTEN
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5L-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11170 Copy
Species: Homo sapiens
Genetic Insert: hATG5
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26812546
Proper citation: RRID:Addgene_111804 Copy
Species: Drosophila melanogaster
Genetic Insert: dAtg1
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23819996
Proper citation: RRID:Addgene_111801 Copy
Species: Drosophila melanogaster
Genetic Insert: dTSC2
Vector Backbone Description: Vector Backbone:pUAST-attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25826530
Proper citation: RRID:Addgene_111808 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
