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http://www.addgene.org/104401

Species: E. coli
Genetic Insert: rpoA, B, C, and Z
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, E. coli expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone

Proper citation: RRID:Addgene_104401 Copy   

•   Source: Addgene

http://www.addgene.org/104404

Species: Homo sapiens
Genetic Insert: TET1
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene plasmid # 47754); Backbone Size:9075; Vector Backbone:pMLM3705; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). TET1 catalytic domain (amino acids 1418- 2136) was from Addgene plasmid 39454 (pAAV-EF1a-HA-hTet1CD- WPRE-PolyA). Use of this plasmid is described in: Baumann, V., Wiesbeck, M., Breunig, C. T., Braun, J. M., Köferle, A., Ninkovic, J., et al. (2019). Targeted removal of epigenetic barriers during transcriptional reprogramming. Nature Communications, 1–12. http://doi.org/10.1038/s41467-019-10146-8

Proper citation: RRID:Addgene_104404 Copy   

•   Source: Addgene

http://www.addgene.org/104403

Species: Homo sapiens
Genetic Insert: DNMT3a
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene plasmid # 47754); Backbone Size:9075; Vector Backbone:pMLM3705; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). DNMT3a (amino acids 598- 908) was from Addgene plasmid 36941 (pcDNA3/Myc-DNMT3A2)

Proper citation: RRID:Addgene_104403 Copy   

•   Source: Addgene

http://www.addgene.org/104381

Species: Homo sapiens
Genetic Insert: SLC30A10
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28883040

Proper citation: RRID:Addgene_104381 Copy   

•   Source: Addgene

http://www.addgene.org/104375

Vector Backbone Description: Backbone Marker:Zhang Lab; Vector Backbone:lentiguide puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29224777
Comments: Follow Zhang Lab protocol for lentiguide-puro cloning

Proper citation: RRID:Addgene_104375 Copy   

•   Source: Addgene

http://www.addgene.org/104372

Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Zhang Lab; Vector Backbone:dcas9-vp64; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29224777
Comments: Based on dCas9-VP64 vector from Zhang Lab

Proper citation: RRID:Addgene_104372 Copy   

•   Source: Addgene

http://www.addgene.org/104415

Species: Homo sapiens
Genetic Insert: dSET7
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene plasmid # 47754); Backbone Size:9075; Vector Backbone:pMLM3705; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Backbone modification: added Hygromycin resistance cassette (PGK promoter and Hygromycin resistance gene from Addgene Plasmid 41721 (MSCVhygro- F-G9a), SV40 early polyadenylation signal from the pCDNA3.1 backbone of Addgene Plasmid 13820 (HDAC1 Flag). SET7/9 (a.a. 52 - 366) was from Addgene plasmid 24082 (pET28 Set9 wt), H297G mutation was added by PCR

Proper citation: RRID:Addgene_104415 Copy   

•   Source: Addgene

http://www.addgene.org/104465

Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29358076

Proper citation: RRID:Addgene_104465 Copy   

•   Source: Addgene

http://www.addgene.org/104468

Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29358076

Proper citation: RRID:Addgene_104468 Copy   

•   Source: Addgene

http://www.addgene.org/104467

Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pTHMT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29358076

Proper citation: RRID:Addgene_104467 Copy   

•   Source: Addgene

http://www.addgene.org/104349

Species: Homo sapiens
Genetic Insert: CD95L leucine zipper
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32706785
Comments: Synthetic DNA encoding a fusion of an improved IL2 signal sequence (Zhang et al. 2005 & Naito et al. 2013), an octahistidine-tagged-leucine zipper motif, a flexible 17aa linker (Naito et al. 2013) and the death inducing, c-terminal extracellular domain (aa137-208) of CD95L. The cloning strategy omits the proteolytically-susceptible extracellular stalk region (aa102-136), but includes the extracellular self-assembly domain (aa137-183; Orlinick et al. 1997). NTA column purified LZ-CD95L produced in expi393 cells is highly active (IC50 Jurkat E6-1 cells < 50ng/ml). Human CD95L is also active in inducing cell death in murine thymocytes (McLellan et al. 2000). Note, during following transient transfection, rising levels of LZ-CD95L in the culture supernatant induce cell death in HEK293FT and expi293. Users could explore co-transfection of this vector with a c-FLIP-encoding vector, select CD95L resistant HEK293 variants, or use other CD95L-resistant mammalian cell lines to maximise LZ-CD95L yields. References: McLellan AD, Terbeck G, Mengling T, Starling GC, Kiener PA, Gold R, Bröcker EB, Leverkus M, Kämpgen E. Differential susceptibility to CD95 (Apo-1/Fas) and MHC class II-induced apoptosis during murine dendritic cell development. Cell Death Differ. 2000 Oct;7(10):933-8. Naito M, Hainz U, Burkhardt UE, Fu B, Ahove D, Stevenson KE, Rajasagi M, Zhu B, Alonso A, Witten E, Matsuoka K, Neuberg D, Duke-Cohan JS, Wu CJ, and Freeman GJ. CD40L-Tri, a novel formulation of recombinant human CD40L that effectively activates B cells. Cancer Immunol Immunother 2013; 62:347-357. Orlinick JR, Elkon KB, and Chao MV. Separate domains of the human fas ligand dictate self-association and receptor binding. J Biol Chem 1997; 272:32221-32229. Zhang L, Leng Q, and Mixson AJ. Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo. The Journal of Gene Medicine 2005; 7:354-365.

Proper citation: RRID:Addgene_104349 Copy   

•   Source: Addgene

http://www.addgene.org/104348

Species: Homo sapiens
Genetic Insert: Human IL-2 (C125S)
Vector Backbone Description: Backbone Marker:qIAGEN; Backbone Size:4751; Vector Backbone:pQE80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Like the FDA approved Proleukin (Aldesleukin), the sequence contains a C125S mutation (TGT -> TCC) to prevent cysteine mispairing in E. coli, but this does not affect biological activity (Wang et al. 1984) Expression and inclusion body preparation was carried out essentially as described in Carmenate et al. (2013), except that the 6M guanidine solubilised IL-2 was applied directly to an NTA column at 1 column volume / minute. LPS was removed with 0.1% Triton X114 / TE washes following by on-column re-folding with TE buffer. NTA-purified His-IL-2 exhibited identical activity to commercially sourced (tag-free) IL-2 (Peprotech). References: Wang A, Lu SD, and Mark DF. Site-specific mutagenesis of the human interleukin-2 gene: structure-function analysis of the cysteine residues. Science 1984; 224:1431-1433. Carmenate T, Pacios A, Enamorado M, Moreno E, Garcia-Martinez K, Fuente D, and Leon K. Human IL-2 mutein with higher antitumor efficacy than wild type IL-2. J. Immunol. 2013; 190:6230-6238.

Proper citation: RRID:Addgene_104348 Copy   

•   Source: Addgene

http://www.addgene.org/104480

Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Vector Backbone:pJ4M; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29438978

Proper citation: RRID:Addgene_104480 Copy   

•   Source: Addgene

http://www.addgene.org/104353

Species: Homo sapiens
Genetic Insert: Human integrin b2 with tension sensor
Vector Backbone Description: Backbone Size:5596; Vector Backbone:pcDNA3.1/Hygro(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27721490
Comments: This plasmid is identical to Plasmid 103877: b2-TS3 (https://www.addgene.org/103877/)

Proper citation: RRID:Addgene_104353 Copy   

•   Source: Addgene

http://www.addgene.org/104355

Species: Homo sapiens
Genetic Insert: Human integrin b2 with tension sensor
Vector Backbone Description: Backbone Size:5596; Vector Backbone:pcDNA3.1/Hygro(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27721490

Proper citation: RRID:Addgene_104355 Copy   

•   Source: Addgene

http://www.addgene.org/104476

Species: Homo sapiens
Genetic Insert: FRBx2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBlueScript KS (-); Vector Types:phagemid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26709839

Proper citation: RRID:Addgene_104476 Copy   

•   Source: Addgene

http://www.addgene.org/104471

Species: Saccharomyces cerevisiae
Genetic Insert: OM45p
Vector Backbone Description: Backbone Size:3545; Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26709839

Proper citation: RRID:Addgene_104471 Copy   

•   Source: Addgene

http://www.addgene.org/104470

Species: Homo sapiens
Genetic Insert: hnRNPA2
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29358076

Proper citation: RRID:Addgene_104470 Copy   

•   Source: Addgene

http://www.addgene.org/104479

Species: Homo sapiens
Genetic Insert: TDP-43
Vector Backbone Description: Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29438978

Proper citation: RRID:Addgene_104479 Copy   

•   Source: Addgene

http://www.addgene.org/104563

Species: Lachnospiraceae bacterium
Genetic Insert: dLbCpf1 (D832A)
Vector Backbone Description: Backbone Marker:Cepko lab (Addgene plasmid # 11179); Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29083402

Proper citation: RRID:Addgene_104563 Copy   

•   Source: Addgene