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Species: S. pyogenes
Genetic Insert: E. coli CRISPR array
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Integration substrate; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27595346
Proper citation: RRID:Addgene_113252 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113240 Copy
Species: E. coli
Genetic Insert: IHFa F13A
Vector Backbone Description: Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113244 Copy
Species: S. pyogenes
Genetic Insert: S. pyogenes Cas1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pET16bMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27595346
Proper citation: RRID:Addgene_113248 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11317 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11318 Copy
Vector Backbone Description: Backbone Size:7661; Vector Backbone:pDM1203; Vector Types:Dictyostelium discoideum expression vector, extrachromosomal; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Proper citation: RRID:Addgene_113196 Copy
Genetic Insert: 2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid.
The GFP binding sites were inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11323 Copy
Genetic Insert: 2 binding sites for let-7a miRNA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: 2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid.
Proper citation: RRID:Addgene_11325 Copy
Vector Backbone Description: Backbone Size:7685; Vector Backbone:pDM1203; Vector Types:Dictyostelium discoideum expression vector, extrachromosomal; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113198 Copy
Species: Synthetic
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Size:6202; Vector Backbone:pDM1501; Vector Types:safe haven knock-in plasmid for the act5 locus,LifeAct-GFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Proper citation: RRID:Addgene_113231 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113237 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113236 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113235 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113239 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113184 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113183 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113182 Copy
Vector Backbone Description: Backbone Size:5554; Vector Backbone:BlueScriptII; Vector Types:generic knockin vector for C-terminal tagging with GFP; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29847546
Proper citation: RRID:Addgene_113222 Copy
Species: E. coli
Genetic Insert: Cas1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:28729350
Proper citation: RRID:Addgene_113188 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
