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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCRISPR
 
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RRID:Addgene_113252 E. coli CRISPR array S. pyogenes Ampicillin PMID:27595346 Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Integration substrate; Bacterial Resistance:Ampicillin 2026-09-12 02:15:30 0
pAW218
 
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RRID:Addgene_113240 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-12 02:15:30 0
pAW204
 
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RRID:Addgene_113244 IHFa F13A E. coli Chloramphenicol PMID:28729350 Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol F13A 2026-09-12 02:15:30 0
pAW-SpyCas1
 
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RRID:Addgene_113248 S. pyogenes Cas1 S. pyogenes Ampicillin PMID:27595346 Backbone Marker:Novagen; Backbone Size:6000; Vector Backbone:pET16bMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:15:30 0
pRL-TK 4x mut D
 
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RRID:Addgene_11317 4 bulged binding sites for CXCR4 siRNA antisense Ampicillin PMID:15014042 Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away. Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin Mutation D (see paper) on inner 2 binding sites. 2026-09-12 02:15:29 0
pRL-TK 4x mut E
 
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RRID:Addgene_11318 4 bulged binding sites for CXCR4 siRNA antisense Ampicillin PMID:15014042 Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away. Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin Mutation E (see paper) on inner 2 binding sites. 2026-09-12 02:15:29 0
pPI459
 
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RRID:Addgene_113196 Ampicillin PMID:29847546 Backbone Size:7661; Vector Backbone:pDM1203; Vector Types:Dictyostelium discoideum expression vector, extrachromosomal; Bacterial Resistance:Ampicillin 2026-09-12 02:15:29 0
pRL-TK 4x mut GFP
 
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RRID:Addgene_11323 2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP Ampicillin PMID:15014042 Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The GFP binding sites were inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-09-12 02:15:30 0
pRL-TK let7 B
 
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1+ mentions
RRID:Addgene_11325 2 binding sites for let-7a miRNA Ampicillin PMID:15014042 2 let-7a sites were inserted into the XbaI site in the 3' UTR of the pRL-TK plasmid. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin Mutation B from figure 4A of paper. 2026-09-12 02:15:30 3
pPI142
 
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RRID:Addgene_113198 Ampicillin Backbone Size:7685; Vector Backbone:pDM1203; Vector Types:Dictyostelium discoideum expression vector, extrachromosomal; Bacterial Resistance:Ampicillin 2026-09-12 02:15:29 0
pPI227
 
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RRID:Addgene_113231 LifeAct Synthetic Ampicillin PMID:29847546 Backbone Size:6202; Vector Backbone:pDM1501; Vector Types:safe haven knock-in plasmid for the act5 locus,LifeAct-GFP; Bacterial Resistance:Ampicillin 2026-09-12 02:15:30 0
pAW215
 
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RRID:Addgene_113237 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-12 02:15:30 0
pAW214
 
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RRID:Addgene_113236 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-12 02:15:30 0
pAW213
 
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RRID:Addgene_113235 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-12 02:15:30 0
pAW217
 
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RRID:Addgene_113239 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-12 02:15:30 0
pAW186
 
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RRID:Addgene_113184 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R163A 2026-09-12 02:15:29 0
pAW151
 
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RRID:Addgene_113183 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R146A 2026-09-12 02:15:29 0
pAW150
 
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RRID:Addgene_113182 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin S143A 2026-09-12 02:15:29 0
pPI180
 
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RRID:Addgene_113222 Ampicillin PMID:29847546 Backbone Size:5554; Vector Backbone:BlueScriptII; Vector Types:generic knockin vector for C-terminal tagging with GFP; Bacterial Resistance:Ampicillin 2026-09-12 02:15:30 0
pAW209
 
Resource Report
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RRID:Addgene_113188 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-12 02:15:29 0

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