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Vector Backbone Description: Vector Backbone:pTargetF_lac_sacB-1-m; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29946130
Comments: Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint.
Proper citation: RRID:Addgene_113135 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two original binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Flanking CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The inner binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11313 Copy
Genetic Insert: 3 perfect binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12600936
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA
stop codon, followed by T, then the XbaI site TCTAGA):
GATCGCCGTG TAA T TCTAGA GTCGGGGCG.
We digested the plasmids with XbaI, and ligated in the following:
ctaga ttccgagatatcggtaat gggcc.
This produced a modified vector, still containing an XbaI site, but
now also containing an ApaI site (GGGCCC), to allow for directional
cloning of 3' UTR inserts. The final vector contains, starting with
the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA.
All UTR inserts were then constructed in the following format:
TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC.
A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG.
The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.
Proper citation: RRID:Addgene_11312 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11315 Copy
Genetic Insert: 4 bulged binding sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15014042
Comments: Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away.
Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites.
See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
Proper citation: RRID:Addgene_11314 Copy
Species: Synthetic
Genetic Insert: T2A-H2B-mCherry
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113089 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30122535
Proper citation: RRID:Addgene_113122 Copy
Species: Homo sapiens
Genetic Insert: p21 Activated kinase 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29983868
Proper citation: RRID:Addgene_113088 Copy
Species: Escherichia coli
Genetic Insert: Rho
Vector Backbone Description: Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30122535
Proper citation: RRID:Addgene_113121 Copy
Genetic Insert: 1 perfect binding site for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12600936
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA
stop codon, followed by T, then the XbaI site TCTAGA):
GATCGCCGTG TAA T TCTAGA GTCGGGGCG.
We digested the plasmids with XbaI, and ligated in the following:
ctaga ttccgagatatcggtaat gggcc.
This produced a modified vector, still containing an XbaI site, but
now also containing an ApaI site (GGGCCC), to allow for directional
cloning of 3' UTR inserts. The final vector contains, starting with
the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA.
All UTR inserts were then constructed in the following format:
TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC.
A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG.
The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.
Proper citation: RRID:Addgene_11310 Copy
Species: Mus musculus
Genetic Insert: Pdgfra donor region including homology arms and T2A-H2B-eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113120 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30122535
Proper citation: RRID:Addgene_113124 Copy
Species: Homo sapiens
Genetic Insert: p21 Activated kinase 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29983868
Proper citation: RRID:Addgene_113090 Copy
Species: Synthetic
Genetic Insert: GINKO1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30652129
Proper citation: RRID:Addgene_113112 Copy
Species: Homo sapiens
Genetic Insert: RapGEF3
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11801596
Proper citation: RRID:Addgene_113110 Copy
Species: Mus musculus
Genetic Insert: Gata4 donor region including homology arms and eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113115 Copy
Species: Mus musculus
Genetic Insert: Cdk9 donor region including homology arms and HaloTag
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113113 Copy
Species: Mus musculus
Genetic Insert: Gata3 donor region including homology arms and T2A-H2B-eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113119 Copy
Species: Mus musculus
Genetic Insert: Dppa1 donor region including homology arms and T2A-H2B-Halo
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113118 Copy
Species: Mus musculus
Genetic Insert: Fgfr2 donor region including homology arms and T2A-H2B-Halo
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113117 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
