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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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E.coli_GW-sgRNA_Lib2 Resource Report Resource Website |
RRID:Addgene_113135 | Ampicillin | PMID:29946130 | Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint. | Vector Backbone:pTargetF_lac_sacB-1-m; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:29 | 0 | |||
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pRL-TK 4x wt Resource Report Resource Website 10+ mentions |
RRID:Addgene_11313 | 4 bulged binding sites for CXCR4 siRNA antisense | Ampicillin | PMID:15014042 | Two original binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away. Flanking CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The inner binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper. | Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:29 | 16 | ||
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pGL3 CXCR4 3P Resource Report Resource Website |
RRID:Addgene_11312 | 3 perfect binding sites for CXCR4 siRNA antisense | Ampicillin | PMID:12600936 | The end of the ORF and the beginning of the 3' UTR is (with the TAA stop codon, followed by T, then the XbaI site TCTAGA): GATCGCCGTG TAA T TCTAGA GTCGGGGCG. We digested the plasmids with XbaI, and ligated in the following: ctaga ttccgagatatcggtaat gggcc. This produced a modified vector, still containing an XbaI site, but now also containing an ApaI site (GGGCCC), to allow for directional cloning of 3' UTR inserts. The final vector contains, starting with the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA. All UTR inserts were then constructed in the following format: TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC. A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG. The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU. | Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 0 | ||
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pRL-TK 4x mut B Resource Report Resource Website |
RRID:Addgene_11315 | 4 bulged binding sites for CXCR4 siRNA antisense | Ampicillin | PMID:15014042 | Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away. Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper. | Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | Mutation B (see paper) on inner 2 binding sites. | 2026-09-12 02:15:29 | 0 | |
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pRL-TK 4x mut A Resource Report Resource Website |
RRID:Addgene_11314 | 4 bulged binding sites for CXCR4 siRNA antisense | Ampicillin | PMID:15014042 | Two identical mutant binding sites, separated by 4 nt, were flanked by two of the orginal bulged binding sites, each 11 nt away. Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The mutant binding sites were then inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper. | Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | Mutation A (see paper) on inner 2 binding sites. | 2026-09-12 02:15:29 | 0 | |
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EasyFusion T2A-H2B-mCherry Resource Report Resource Website |
RRID:Addgene_113089 | T2A-H2B-mCherry | Synthetic | Ampicillin | PMID:29889212 | Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 0 | ||
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EcNusGFL Resource Report Resource Website |
RRID:Addgene_113122 | NusG | Escherichia coli | Kanamycin | PMID:30122535 | Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:15:28 | 0 | ||
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PAK4-FL-3'UTR Resource Report Resource Website 1+ mentions |
RRID:Addgene_113088 | p21 Activated kinase 4 | Homo sapiens | Ampicillin | PMID:29983868 | Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | none | 2026-09-12 02:15:28 | 1 | |
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EcRho Resource Report Resource Website |
RRID:Addgene_113121 | Rho | Escherichia coli | Kanamycin | PMID:30122535 | Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:15:28 | 0 | ||
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pGL3 CXCR4 1P Resource Report Resource Website 1+ mentions |
RRID:Addgene_11310 | 1 perfect binding site for CXCR4 siRNA antisense | Ampicillin | PMID:12600936 | The end of the ORF and the beginning of the 3' UTR is (with the TAA stop codon, followed by T, then the XbaI site TCTAGA): GATCGCCGTG TAA T TCTAGA GTCGGGGCG. We digested the plasmids with XbaI, and ligated in the following: ctaga ttccgagatatcggtaat gggcc. This produced a modified vector, still containing an XbaI site, but now also containing an ApaI site (GGGCCC), to allow for directional cloning of 3' UTR inserts. The final vector contains, starting with the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA. All UTR inserts were then constructed in the following format: TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC. A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG. The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU. | Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 3 | ||
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Pdgfra-T2A-H2B-eGFP donor Resource Report Resource Website |
RRID:Addgene_113120 | Pdgfra donor region including homology arms and T2A-H2B-eGFP | Mus musculus | Ampicillin | PMID:29889212 | Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 0 | ||
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EcNusGKOW Resource Report Resource Website |
RRID:Addgene_113124 | NusG | Escherichia coli | Kanamycin | PMID:30122535 | Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Deleted residues 1-124 | 2026-09-12 02:15:29 | 0 | |
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PAK4-3'UTR-BS1 Resource Report Resource Website |
RRID:Addgene_113090 | p21 Activated kinase 4 | Homo sapiens | Ampicillin | PMID:29983868 | Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | none | 2026-09-12 02:15:28 | 0 | |
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pcDNA3.1-GINKO1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_113112 | GINKO1 | Synthetic | Ampicillin | PMID:30652129 | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 2 | ||
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pEYFP-N3-EPAC1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_113110 | RapGEF3 | Homo sapiens | Kanamycin | PMID:11801596 | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-09-12 02:15:28 | 1 | ||
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Gata4-GFP donor Resource Report Resource Website |
RRID:Addgene_113115 | Gata4 donor region including homology arms and eGFP | Mus musculus | Ampicillin | PMID:29889212 | Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 0 | ||
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Cdk9-Halo donor Resource Report Resource Website |
RRID:Addgene_113113 | Cdk9 donor region including homology arms and HaloTag | Mus musculus | Ampicillin | PMID:29889212 | Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 0 | ||
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Gata3-T2A-H2B-eGFP donor Resource Report Resource Website 1+ mentions |
RRID:Addgene_113119 | Gata3 donor region including homology arms and T2A-H2B-eGFP | Mus musculus | Ampicillin | PMID:29889212 | Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 1 | ||
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Dppa1-T2A-H2B-Halo donor Resource Report Resource Website |
RRID:Addgene_113118 | Dppa1 donor region including homology arms and T2A-H2B-Halo | Mus musculus | Ampicillin | PMID:29889212 | Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 0 | ||
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Fgfr2-T2A-H2B-Halo donor Resource Report Resource Website |
RRID:Addgene_113117 | Fgfr2 donor region including homology arms and T2A-H2B-Halo | Mus musculus | Ampicillin | PMID:29889212 | Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:28 | 0 |
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
