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Vector Backbone Description: Backbone Size:9697; Vector Backbone:pGreenFire[; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37857935
Proper citation: RRID:Addgene_174103 Copy
Species: Synthetic
Genetic Insert: Tetra Cysteine tag
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39554417
Proper citation: RRID:Addgene_221852 Copy
Vector Backbone Description: Backbone Size:4917; Vector Backbone:pSpy1C; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: We took the rolling circle replicon pSH71 and the chloramphenicol resistance cassette from pIB166 (Plasmid #90189). The the Plac_mrfp cassette for the MCS is derived from pBS3Clux (Plasmid #55172).
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220248 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39554417
Proper citation: RRID:Addgene_221850 Copy
Species: Rotavirus SA11
Genetic Insert: Rotavirus SA11 NSP3-F2A-EGFP
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_220808 Copy
Species: Neonothopanus nambi
Genetic Insert: nnLuz-I{PIV-216}
Vector Backbone Description: Vector Backbone:pLSU1; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_212192 Copy
Species: Neonothopanus nambi
Genetic Insert: nnLuz-I{PIV-216}
Vector Backbone Description: Vector Backbone:pLSU1; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_212191 Copy
Species: Homo sapiens
Genetic Insert: Breast Cancer 1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pFastbac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37797621
Proper citation: RRID:Addgene_223228 Copy
Genetic Insert: Nucleoplasmin NLS
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47986); Backbone Size:2845; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B2, insert can be released via BsaI digest
Proper citation: RRID:Addgene_217998 Copy
Species: Synthetic
Genetic Insert: Start-Met
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47986); Backbone Size:2845; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B2, insert can be released via BsaI digest
Proper citation: RRID:Addgene_217999 Copy
Species: Synthetic
Genetic Insert: Strep-8xHis
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47989); Backbone Size:2845; Vector Backbone:pAGM1301; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B5, insert can be released via BsaI digest
Plasmid used in the MoClo toolkit for Chlamydomonas reinhardtii from Crozet et al. (2018)
Proper citation: RRID:Addgene_218201 Copy
Species: Leishmania tarentolae
Genetic Insert: smtHSP60
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47986); Backbone Size:2845; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B2, insert can be released via BsaI digest
Proper citation: RRID:Addgene_217997 Copy
Vector Backbone Description: Backbone Marker:self-made; Backbone Size:7710; Vector Backbone:derived from pLEXSY_I-blecherry3 (Jena Bioscience); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38799406
Comments: MoClo L1 destination vector
Proper citation: RRID:Addgene_217994 Copy
Species: Leishmania mexicana
Genetic Insert: Secretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47986); Backbone Size:2845; Vector Backbone:pAGM1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B2, insert can be released via BsaI digest
Proper citation: RRID:Addgene_217995 Copy
Species: Synthetic
Genetic Insert: 14xGCN4pep
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211877 Copy
Species: Synthetic
Genetic Insert: 12xGCN4pep
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211876 Copy
Species: Synthetic
Genetic Insert: 10xGCN4pep
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211875 Copy
Species: Synthetic
Genetic Insert: 6xGCN4pep
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211873 Copy
Species: Synthetic
Genetic Insert: 4xGCN4pep
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211872 Copy
Genetic Insert: pCAGGS/EG5-RBD
Vector Backbone Description: Backbone Size:4745; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38405707
Proper citation: RRID:Addgene_213410 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
