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Species: Rotavirus SA11
Genetic Insert: Rotavirus SA11 NSP3-P2A-C1C-EGFP
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_220810 Copy
Species: Rotavirus DS-1
Genetic Insert: Rotavirus DS-1 NSP1(ioSA11)
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_220818 Copy
Species: Rotavirus DS-1
Genetic Insert: Rotavirus DS-1 NSP1
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_220817 Copy
Species: Rotavirus SA11
Genetic Insert: Rotavirus SA11 NSP1delta103C-GFP
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_220813 Copy
Species: Homo sapiens
Genetic Insert: overexpression library with around 450 codon-optimized Human transporter cDNAs
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Vector Backbone:pLIX_401; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39755121
Proper citation: RRID:Addgene_213694 Copy
Species: Synthetic
Genetic Insert: AAV-BI156 Cap
Vector Backbone Description: Vector Backbone:iCap-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39097583
Proper citation: RRID:Addgene_209528 Copy
Species: Synthetic
Genetic Insert: Strep-tag II
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47987); Backbone Size:2845; Vector Backbone:pICH41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B3, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218000 Copy
Vector Backbone Description: Backbone Size:5256; Vector Backbone:pSpy0C4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: The multiple cloning site (EcoRI, XbaI, SpeI and PstI) contains the Plac_mrfp cassette. The mrfp reporter allows for red-white screening during sub-cloning in E. coli (DH5a). When the Plac_mrfp cassette is replaced with the insert of interest, colonies will appear white.
Resource Information: The MCS featuring the Plac_mrfp cassette and the chloramphenicol resistance cassette were taken from pSpy1C. The pUC origin for replication in E. coli was taken from pUC19-mKate2∼ssrA. Up- and downstream homologous regions of sagB were amplified from the S. pyogenes SF370 genome.
Linearise the plasmid with the restriction enzyme Alw44I before transformation in S. pyogenes to favour double crossover recombination of the plasmid into the sagB locus. Positive transformants will show loss of hemolysis on TSA blood agar plates.
Please download the file linked below to obtain a well annotated map of the plasmid.
Proper citation: RRID:Addgene_220280 Copy
Species: Synthetic
Genetic Insert: AAV-BI153 Cap
Vector Backbone Description: Vector Backbone:iCap-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39097583
Proper citation: RRID:Addgene_209525 Copy
Species: Synthetic
Genetic Insert: AAV-BI154 Cap
Vector Backbone Description: Vector Backbone:iCap-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39097583
Proper citation: RRID:Addgene_209526 Copy
Species: Synthetic
Genetic Insert: AAV-BI151 Cap
Vector Backbone Description: Vector Backbone:iCap-Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39097583
Proper citation: RRID:Addgene_209523 Copy
Species: Photinus pyralis
Genetic Insert: ffluc
Vector Backbone Description: Vector Backbone:pSpy1C; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38911550
Comments: This high-copy replicative shuttle plasmid features an erythromycin-inducible riboswitch driving expression of the Firefly luciferase in the presence of erythromycin. Luminescence can be detected by adding luciferin to the culture. See associated publication for more details.
Resource Information: The erythromycin-inducible riboswitch was taken from pLZ12Km2-TA:PgyrA(Sag)-ermBL-ermB':A2I:ffluc-tt3, which was used in the following publication:
Dynamics of diversified A-to-I editing in Streptococcus pyogenes is governed by changes in mRNA stability
Thomas F. Wulff, Karin Hahnke, Anne-Laure Lécrivain, Katja Schmidt, Rina Ahmed-Begrich, Knut Finstermeier, Emmanuelle Charpentier
bioRxiv 2023.09.19.555891; doi: https://doi.org/10.1101/2023.09.19.555891
The ffluc reporter gene was taken from the original pLZ12Km2-P23R:TA:ffluc plasmid (Plasmid #88900).
Please download the detailed plasmid map using the file linked below
Proper citation: RRID:Addgene_218513 Copy
Species: SARS-CoV-2
Genetic Insert: RBD
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47988); Backbone Size:2845; Vector Backbone:pAGM1299; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B4, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218010 Copy
Species: Synthetic
Genetic Insert: mCerulean
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47996); Backbone Size:2845; Vector Backbone:pAGM1287; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B3-B4, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218009 Copy
Species: Synthetic
Genetic Insert: Spacer
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47987); Backbone Size:2845; Vector Backbone:pICH41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B3, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218006 Copy
Species: Synthetic
Genetic Insert: ER retention signal (DGDL)
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47989); Backbone Size:2845; Vector Backbone:pAGM1301; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B5, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218021 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47988); Backbone Size:2845; Vector Backbone:pAGM1299; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B4, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218014 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47988); Backbone Size:2845; Vector Backbone:pAGM1299; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B4, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218013 Copy
Genetic Insert: 8xHis-1xHA-tag
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47989); Backbone Size:2845; Vector Backbone:pAGM1301; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B5, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218019 Copy
Species: Synthetic
Genetic Insert: mVenus
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet (Addgene plasmid #47989); Backbone Size:2845; Vector Backbone:pAGM1301; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38799406
Comments: Level 0 MoClo Part Position B5, insert can be released via BsaI digest
Proper citation: RRID:Addgene_218016 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
