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Species: Clavibacter michiganensis
Genetic Insert: cspA2
Vector Backbone Description: Backbone Size:4777; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38814867
Comments: Please visit https://pubmed.ncbi.nlm.nih.gov/37790530/ for bioRxiv preprint.
Proper citation: RRID:Addgene_215399 Copy
Species: West Nile
Genetic Insert: West Nile NS2B-NS3 fusion
Vector Backbone Description: Vector Backbone:pNIC-NHStIIT; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://asapdiscovery.org/outputs/target-enabling-packages/
Proper citation: RRID:Addgene_204794 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YMR253C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175221 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YNL095C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175222 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YOL092W (CEN.PK strain)
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175224 Copy
Species: Homo sapiens
Genetic Insert: OpenCRISPR-1 sgRNA 16nt stem loop
Vector Backbone Description: Backbone Marker:Twist; Backbone Size:4303; Vector Backbone:pTwist CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40739342
Comments: Please visit https://doi.org/10.1101/2024.04.22.590591 for bioRxiv preprint.
Proper citation: RRID:Addgene_221566 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YLR152C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175217 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2953; Vector Backbone:pACYC177; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38180814
Proper citation: RRID:Addgene_207954 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YHR032W
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175210 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YDL231C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: YDR338C
Vector Backbone Description: Backbone Size:3099; Vector Backbone:pNB1u/pLIFE016; Vector Types:mRNA expression, injection into xenopus oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38680917
Comments: Generated by USER cloning
Proper citation: RRID:Addgene_175212 Copy
Species: Clitoria ternatea
Genetic Insert: Butelase 1 ligase
Vector Backbone Description: Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39614317
Proper citation: RRID:Addgene_221848 Copy
Species: Clitoria ternatea
Genetic Insert: Butelase 1 ligase
Vector Backbone Description: Vector Backbone:pHYRSF53; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39614317
Proper citation: RRID:Addgene_221847 Copy
Species: Homo sapiens
Genetic Insert: ATP8B2
Vector Backbone Description: Vector Backbone:pMx; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39219493
Proper citation: RRID:Addgene_209227 Copy
Vector Backbone Description: Backbone Marker:Used yCP50-poly backbone for yCP50-poly-KmR and yTREX construction; Backbone Size:10548; Vector Backbone:yCP50-poly-KmR; Vector Types:Bacterial Expression, Synthetic Biology, Yeast Expression, Tn5 genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29552656
Comments: The vector enables chromosomal integration via random transposition, facilitated by transposon Tn5 elements (tnp gene as well as outside end sequences OE-L and OE-R), which define the integron - including P-T7 promoters and a TcR marker. Target genes can be inserted at the I-SceI site. The vector backbone is derived from yCP50-poly (Wang and Stillman 1993, PMID: 8441414), which was obtained from ATCC (87555) via LGC Standards GmbH, (see Domrose et al. 2017, PMID: 29552656), and is equipped on the one hand with the bacterial pMB1 ori, aphII-encoded KmR and mob gene from pBBR1 including its promoter and oriT region, and on the other hand with yeast replication elements CEN4-ARS1 as well as the URA3 marker.
Proper citation: RRID:Addgene_177306 Copy
Species: Homo sapiens
Genetic Insert: ATP9B
Vector Backbone Description: Vector Backbone:pMx; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40234049
Proper citation: RRID:Addgene_209231 Copy
Species: Rotavirus
Genetic Insert: Rotavirus NSP5SClow
Vector Backbone Description: Vector Backbone:pT7; Vector Types:Other; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_220829 Copy
Species: Homo sapiens
Genetic Insert: Beta-1,3-N-Acetylglucosaminyltransferase 5
Vector Backbone Description: Backbone Marker:addgene 52962; Backbone Size:8669; Vector Backbone:lentiCas9-blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37577488
Comments: Please visit https://doi.org/10.1101/2023.08.02.551727 for bioRxiv preprint.
Proper citation: RRID:Addgene_208380 Copy
Species: Escherichia coli
Genetic Insert: LacI
Vector Backbone Description: Backbone Size:7007; Vector Backbone:pIA034; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39494880
Comments: pPT300 was amplified using primers oIA027 and oIA069. The resulting fragment was cloned into pIA034 digested with BamHI and NotI, giving pIA036, amyE::(Ppen-lacIΔ11-bsdronPA cm).
Please visit https://doi.org/10.1101/2023.03.16.532899 for bioRxiv preprint.
Proper citation: RRID:Addgene_200425 Copy
Genetic Insert: Tras light chain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Affinity Reagent/ Antibody; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38493197
Proper citation: RRID:Addgene_216294 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
