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Species: Homo sapiens
Genetic Insert: p16 INK4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7603984
Comments: A human pl6ink4 cDNA was obtained by PCR amplification of a HeLa cell cDNA library with a 5' primer (5'-GGAATTCACCACCATGGAGCCTTCGGCTGAC-3') and a 3' primer (5'-GGAATTCTCGAGTCAATCGGGGATATCTGAGGGACC-3'). A 472-bp fragment was amplified, purified on a low-melting agarose gel, and cloned directly into pGEM-T (Promega). The resulting plasmid was then used to isolate an EcoRI/Xho I fragment containing the pl6ink4 coding region, which was cloned into pcDNA3 (Invitrogen) to construct pCMV.pl6ink4.
The insert corresponds to isoform 1 but is missing the first 8 amino acids.
Proper citation: RRID:Addgene_10916 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Size:3082; Vector Backbone:pKM464; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating deletion of mycobacterial target genes, using zeocin as a resistance marker.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_109301 Copy
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5711; Vector Backbone:pET-16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30798527
Proper citation: RRID:Addgene_109303 Copy
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4008; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30798527
Proper citation: RRID:Addgene_109304 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109241 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109233 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109234 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109235 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109236 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109237 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109251 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109253 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109254 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109245 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29553576
Proper citation: RRID:Addgene_109249 Copy
Species: Homo sapiens
Genetic Insert: Depdc5
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590090
Proper citation: RRID:Addgene_109341 Copy
Species: Homo sapiens
Genetic Insert: Depdc5
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29590090
Proper citation: RRID:Addgene_109337 Copy
Species: Homo sapiens
Genetic Insert: GαS
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109350 Copy
Species: Homo sapiens
Genetic Insert: Gso chimera
Vector Backbone Description: Backbone Size:6002; Vector Backbone:pEM705; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29338718
Proper citation: RRID:Addgene_109353 Copy
Species: Homo sapiens
Genetic Insert: Cyclin E1
Vector Backbone Description: Vector Backbone:pInducer20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29148972
Proper citation: RRID:Addgene_109348 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
