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Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC19 (Addgene #50005); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_219828 Copy
Species: Ruminococcus flavefaciens
Genetic Insert: RfxCas13d
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE (Addgene #138147); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_219823 Copy
Species: Thermoanaerobacter pseudethanolicus ATCC 33223
Genetic Insert: antiterminator protein LoaP
Vector Backbone Description: Backbone Size:5983; Vector Backbone:pET-19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38959899
Proper citation: RRID:Addgene_222951 Copy
Species: Homo sapiens
Genetic Insert: Luciferase, GFP
Vector Backbone Description: Backbone Marker:Boris Fehse (Addgene #27358); Vector Backbone:LeGO-iG; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Comments: This is an inducible lentiviral luciferase expression vector that has 6 RAS/MAPK-inducible AP-1 sites driving transcription from the minimal CSF2 promoter. LEGO-AP1x6-GM55 was constructed from LEGO-AP1x3-GM55 by duplicating the array of 3 AP-1 sites. LEGO-AP1x3-GM55 was previously constructed from LeGO-iG (Weber et al, 2008) (Addgene #27358) by replacing a NotI/XbaI fragment containing the U6 and SFFV promoters with a fragment from the plasmid pAP1-3 (Addgene #71258) that has 3 copies of the MMP3 gene AP-1 site in front of human CSF2 -55 to +28 minimal promoter driving expression of the Luc+ firefly luciferase gene originating from the plasmid pGL3 (Promega). The luciferase gene is linked to an IRES that in principle supports co-expression of luciferase and EGFP. The insert has 2 copies of the SV40 transcription termination/polyadenylation signal upstream of the multiple cloning site, which blocks background read-through transcription originating in the vector backbone, which itself can be substantial (Bert et al, 2000). The inserted luciferase gene and SV40 polyA site sequences originate from the plasmid pXPG (Bert et al, 2000) (Addgene #71248).
References:
Bert et al Plasmid. 2000 Sep;44(2):173-82. PMID 10964627.
Weber et al Mol Ther. 2008 Apr. 16(4):698-706. PMID 18362927.
Proper citation: RRID:Addgene_217415 Copy
Species: Synthetic
Genetic Insert: CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
Vector Backbone Description: Vector Backbone:pCold; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38014175
Proper citation: RRID:Addgene_222987 Copy
Species: Homo sapiens
Genetic Insert: Cas9, FIGLA sgRNA 1
Vector Backbone Description: Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2022.07.11.499564 for bioRxiv preprint
Proper citation: RRID:Addgene_222906 Copy
Species: Homo sapiens
Genetic Insert: MAP2K1
Vector Backbone Description: Backbone Size:7565; Vector Backbone:pCW107-V5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_217300 Copy
Species: Homo sapiens
Genetic Insert: Cas9, FIGLA sgRNA 4
Vector Backbone Description: Vector Backbone:pX330; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2022.07.11.499564 for bioRxiv preprint
Proper citation: RRID:Addgene_222909 Copy
Species: E. coli
Genetic Insert: IS621 Helper (WT)
Vector Backbone Description: Vector Backbone:pNP1950 - p15A Ori; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:38926615
Comments: Please visit https://doi.org/10.1101/2024.01.24.577089 for bioRxiv preprint.
Proper citation: RRID:Addgene_222732 Copy
Species: Homo sapiens
Genetic Insert: FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4305; Vector Backbone:Modified pcDNA3.1/Hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38536408
Comments: Please visit https://doi.org/10.1101/2023.12.16.571988 for bioRxiv preprint.
Proper citation: RRID:Addgene_222886 Copy
Species: Homo sapiens
Genetic Insert: LHX8
Vector Backbone Description: Vector Backbone:pPB-cT3G-cERH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2022.07.11.499564 for bioRxiv preprint
Proper citation: RRID:Addgene_222921 Copy
Species: Homo sapiens
Genetic Insert: FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4305; Vector Backbone:Modified pcDNA3.1/Hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38536408
Comments: Please visit https://doi.org/10.1101/2023.12.16.571988 for bioRxiv preprint.
Proper citation: RRID:Addgene_222885 Copy
Species: Homo sapiens
Genetic Insert: HHEX
Vector Backbone Description: Vector Backbone:pPB-cT3G-cERP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2022.07.11.499564 for bioRxiv preprint
Proper citation: RRID:Addgene_222928 Copy
Species: P. aeruginosa (Bacteria)
Genetic Insert: feoB
Vector Backbone Description: Vector Backbone:pET41-a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: IPTG inducible
Proper citation: RRID:Addgene_216756 Copy
Species: Homo sapiens
Genetic Insert: ZNF281
Vector Backbone Description: Vector Backbone:pPB-cT3G-cERH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2022.07.11.499564 for bioRxiv preprint
Proper citation: RRID:Addgene_222923 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting human SMCR8
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27559131
Proper citation: RRID:Addgene_83440 Copy
Species: P. aeruginosa (Bacteria)
Genetic Insert: feoB
Vector Backbone Description: Vector Backbone:pET41-a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: IPTG inducible
Proper citation: RRID:Addgene_216754 Copy
Species: Rattus norvegicus
Genetic Insert: DJ-1 shRNA
Vector Backbone Description: Backbone Size:7073; Vector Backbone:pLKO.1 - TRC mTagBFP2; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39167658
Comments: Please visit https://doi.org/10.1101/2023.10.10.561760 for bioRxiv preprint.
Proper citation: RRID:Addgene_222870 Copy
Species: Synthetic
Genetic Insert: His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Vector Backbone Description: Vector Backbone:pCold; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38014175
Proper citation: RRID:Addgene_222991 Copy
Species: Homo sapiens
Genetic Insert: gRNA targeting human C9orf72
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27559131
Proper citation: RRID:Addgene_83439 Copy
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The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
