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Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108372 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108371 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108370 Copy
Species: Mus musculus
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108403 Copy
Species: Mus musculus
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108402 Copy
Species: Homo sapiens
Genetic Insert: Wild type TPI reporter with MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108368 Copy
Species: Homo sapiens
Genetic Insert: Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108366 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Knock-in Cg TDH3 promoter in Cg YAP1 gene
Vector Backbone Description: Backbone Size:2648; Vector Backbone:pFA6a; Vector Types:Replace C.glabrata YAP1 promoter with C. glabrata TDH3 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29600281
Proper citation: RRID:Addgene_108364 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108409 Copy
Species: Homo sapiens
Genetic Insert: PKHD1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17470460
Comments: Addgene sequencing results find V1463A, L1870V, S3864P, and Q3899R mutations in PKHD1 compared to the NCBI reference sequence NM_138694.3. The S3864P mutation is specific to this plasmid from the publication. It is unknown how these mutations affect plasmid function.
Proper citation: RRID:Addgene_108408 Copy
Species: Homo sapiens
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Comments: Note: S999P mutation is present in PC1. It is unknown how this mutation affects plasmid function.
Proper citation: RRID:Addgene_108406 Copy
Species: Synthetic
Genetic Insert: xCas9 3.6
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108384 Copy
Species: Synthetic
Genetic Insert: xCas9(3.7)-BE4
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108381 Copy
Species: Synthetic
Genetic Insert: xCas9 3.7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108379 Copy
Species: Homo sapiens
Genetic Insert: miRFP-H2B
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108411 Copy
Species: Synthetic
Genetic Insert: miRFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:939; Vector Backbone:pBAD-HisD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29483642
Proper citation: RRID:Addgene_108410 Copy
Species: Homo sapiens
Genetic Insert: PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27917860
Comments: Please see depositor's genbank file in Supplemental Documents for full annotation of the plasmid
Proper citation: RRID:Addgene_108376 Copy
Species: Bos taurus
Genetic Insert: p110 KD
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6620; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10860837
Comments: A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR. The kinase dead mutant has an additional BamHI site at D915S.
Proper citation: RRID:Addgene_10837 Copy
Species: Bos taurus
Genetic Insert: p110
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6620; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10860837
Comments: A pair of overlapping oligomers were synthesized based on the c-src myristoylation signal peptide sequence. A Kozak sequence was engineered at the 5 of the c-src myristoylation signal sequence (MetGly-Ser-Ser-Lys-Ser-Lys-Pro-Lys-Asp-Pro-Ser-Gln-Arg-Arg-ArgArg-Ile-Arg-Thr). The fragment was ligated to the 5 end of bovine p110 sequence and cloned into a retroviral expression vector (pLNCX) by a three piece ligation reaction. A 6 histidine tag was incorporated on the 3 end of p110 by PCR.
Proper citation: RRID:Addgene_10836 Copy
Species: Homo sapiens
Genetic Insert: Arf1 Q71L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11773070
Proper citation: RRID:Addgene_10832 Copy
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