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URL: http://www.wormbase.org/db/get?name=WBStrain00062889
Proper Citation: RRID:WB-STRAIN:WBStrain00062889
Description: Caenorhabditis elegans with name daf-16(ot853[daf-16::mNG::AID]) I; otSi2 II. from WB.
Species: Caenorhabditis elegans
Synonyms: daf-16(ot853[daf-16::mNG::AID]) I; otSi2 II.
Notes: otSi2 [ges-1p::TIR1(F79G)::mRuby::unc-54 3'UTR + Cbr-unc-119(+) *ieSi61] II. Intestine-specific TIR1 sequence in ieSi61 allele was edited to TIR1(F79G) using CRISPR/Cas9 to make it compatible with AID2. [TCC GTC GAG CTC AAG GGA AAG CCA CAC TTC] edited to [AGT GTC GAA TTG AAG GGA AAG CCA CAC GGA]. This strain can be used to deplete DAF-16 specifically from the intestine with the modified auxin 5-Ph-IAA. Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https:
Affected Gene: WBGene00000912(daf-16)
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Source: Integrated Animals
Source Database: WormBase (WB)