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URL: http://www.addgene.org/113766
Proper Citation: RRID:Addgene_113766
Insert Name: β subunit of Protocatechuate 3,4- Dioxygenase
Organism: P. putida
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25548185
Vector Backbone Description: Backbone Marker:DNASU plasmid repository, Arizona State University Biodesign Institute; Backbone Size:4010; Vector Backbone:pVP91A vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please ensure that the chosen expression cell line expresses the T5 RNA polymerase. Senavirathne, G., et al., Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging. Anal Biochem, 2018. 556: p. 78-84.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
