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URL: http://www.addgene.org/11323
Proper Citation: RRID:Addgene_11323
Insert Name: 2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15014042
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Two original CXCR4 sites, with XhoI and SpeI restriction sites between them, were inserted into the XbalI site in the 3' UTR of the pRL-TK plasmid. The GFP binding sites were inserted by ligating annealed oligos into the XhoI and SpeI sites. See Addgene plasmid 11307 for cloning of binding sites into the backbone, used in Doench, Sharp 2003 paper.
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Source: Addgene
The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
