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URL: http://www.addgene.org/11310
Proper Citation: RRID:Addgene_11310
Insert Name: 1 perfect binding site for CXCR4 siRNA antisense
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12600936
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-control; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA stop codon, followed by T, then the XbaI site TCTAGA): GATCGCCGTG TAA T TCTAGA GTCGGGGCG. We digested the plasmids with XbaI, and ligated in the following: ctaga ttccgagatatcggtaat gggcc. This produced a modified vector, still containing an XbaI site, but now also containing an ApaI site (GGGCCC), to allow for directional cloning of 3' UTR inserts. The final vector contains, starting with the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA. All UTR inserts were then constructed in the following format: TCTAGACTCGAGCCGG[binding site]ATCGCGGGCCC. A single perfect binding site is AAGTTTTCACTCCAGCTAACACCGG. The CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The data sets included in this resource are published in Kravitz et al, 2012. Distinct roles for direct and indirect pathway striatal neurons in reinforcement (Nat. Neurosci).
