Searching the Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Posttranscriptional splicing of premessenger RNA (mRNA) is an evolutionarily conserved eukaryotic process for producing mature mRNA that is translated into proteins. Accurate splicing is necessary for normal growth and development, and aberrant splicing is increasingly evident in various human pathologies. To study environmental factors that influence RNA splicing, we employed a fluorescent Caenorhabditis elegans in vivo splicing reporter as a biomarker for splicing fidelity to screen against the US EPA ToxCast chemical library. We identified pararosaniline hydrochloride as a strong modifier of RNA splicing. Through gene expression analysis, we found that pararosaniline activates the oxidative stress response and alters the expression of key RNA splicing regulator genes. Physiological assays show that pararosaniline is deleterious to C. elegans development, reproduction, and aging. Through a targeted RNAi screen, we found that inhibiting protein translation can reverse pararosaniline's effect on the splicing reporter and provide significant protection against long-term pararosaniline toxicity. Together, this study reveals a new chemical modifier of RNA splicing and describes translation inhibition as a genetic mechanism to provide resistance.
Pubmed ID: 37852248
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Center that acquires, maintains, and distributes genetic stocks and information about stocks of the small free-living nematode Caenorhabditis elegans for use by investigators initiating or continuing research on this genetic model organism. A searchable strain database, general information about C. elegans, and links to key Web sites of use to scientists, including WormBase, WormAtlas, and WormBook are available.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name (raga-1(ok386) II; lin-15(n765)ybIs2167[eft-3p::ret-1E4E5(+1) E6-GGS6-mCherry+eft-3p::ret-1E4E5(+1) E6(+2)GGS6-GFP+ lin-15(+)+pRG5271Neo] X) from WB.
View all literature mentionsCaenorhabditis elegans with name Caenorhabditis elegans wild isolate. from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsCaenorhabditis elegans with name (raga-1(ok386) II; lin-15(n765)ybIs2167[eft-3p::ret-1E4E5(+1) E6-GGS6-mCherry+eft-3p::ret-1E4E5(+1) E6(+2)GGS6-GFP+ lin-15(+)+pRG5271Neo] X) from WB.
View all literature mentionsCaenorhabditis elegans with name EMPTY from WB.
View all literature mentionsWelcome to the ASWG Community.
Our Information
