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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037162
Source Database: WormBase (WB)
Affected Genes: WBGene00011502(vps-53)
Genomic Alteration: WBGene00011502(vps-53)
Availability: available
Source References: EMPTY
Synonyms: T05G5.8(ok2864) III.
Alternate IDs: WB-STRAIN:VC2202, CGC_VC2202
Notes: Made_by: Vancouver KO Group|"T05G5.8. External left primer: AGAGCAGCATCACAAGTGGA. External right primer: CTGGAAAAGGGGGACAAAAT. Internal left primer: CAACATCCTGAACTAAAACCTGG. Internal right primer: TATGTGTAGAGTGGCGGCTG. Internal WT amplicon: 1123 bp. Deletion size: 373 bp. Deletion left flank: CCAGGAACTCATTTAAAGTTTTCTCTTGAG. Deletion right flank: TTGCTGTTCGATGAACCATTTTACAAAGTT. Insertion Sequence: TATTTATAAATACATCCAAGAAAGTATCAAAAACACTCCAAATAGCTTTTTCGAATGAA A."|"T05G5.8. External left primer: AGAGCAGCATCACAAGTGGA. External right primer: CTGGAAAAGGGGGACAAAAT. Internal left primer: CAACATCCTGAACTAAAACCTGG. Internal right primer: TATGTGTAGAGTGGCGGCTG. Internal WT amplicon: 1123 bp. Deletion size: 373 bp. Deletion left flank: CCAGGAACTCATTTAAAGTTTTCTCTTGAG. Deletion right flank: TTGCTGTTCGATGAACCATTTTACAAAGTT. Insertion Sequence: TATTTATAAATACATCCAAGAAAGTATCAAAAACACTCCAAATAGCTTTTTCGAATGAAA."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037162 Copy
http://www.wormbase.org/db/get?name=WBStrain00037165
Source Database: WormBase (WB)
Affected Genes: WBGene00019087(citk-2)
Genomic Alteration: WBGene00019087(citk-2)
Availability: available
Source References: EMPTY
Synonyms: citk-2(ok2885) II.
Alternate IDs: WB-STRAIN:VC2205, CGC_VC2205
Notes: F59A6.5. External left primer: CAACAACGAATCACACGAGG. External right primer: CCACTTTGCGTTGACTCTCA. Internal left primer: TGGACGCTGAGAACATCATC. Internal right primer: GATTCGAACCACCATCTCGT. Internal WT amplicon: 1323 bp. Deletion size: 693 bp. Deletion left flank: CAAGAACGACATGAAAGAGAACGATCGCAA. Deletion right flank: AACATTTGATTATGGCCGACCAACAGTGTT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037165 Copy
http://www.wormbase.org/db/get?name=WBStrain00037164
Source Database: WormBase (WB)
Affected Genes: WBGene00006845(unc-122)
Genomic Alteration: WBGene00006845(unc-122)
Availability: available
Source References: EMPTY
Synonyms: unc-122(ok2882) I.
Alternate IDs: WB-STRAIN:VC2204, CGC_VC2204
Notes: F11C3.2. External left primer: CCCATTCACATTTTCAGGCT. External right primer: TGCCGCACACCAATAATAAA. Internal left primer: CCGGCGAAATAGGAAATGTA. Internal right primer: ACTTCCTGCGGAAGAAACCT. Internal WT amplicon: 1145 bp. Deletion size: 327 bp. Deletion left flank: TGATCACAAAAATCAAGAACTCTCAGAGAA. Deletion right flank: GAAAAAATGGTTCCTGTGCCAGTAGTGGTT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037164 Copy
http://www.wormbase.org/db/get?name=WBStrain00037125
Source Database: WormBase (WB)
Affected Genes: WBGene00001127(dyf-11)
Genomic Alteration: WBGene00001127(dyf-11)
Availability: available
Source References: EMPTY
Synonyms: dyf-11(ok2926) X.
Alternate IDs: WB-STRAIN:VC2161, CGC_VC2161
Notes: C02H7.1. External left primer: TTCAAGGAATGGTACCGGAG. External right primer: GGGCATTTCCAAGTTTTTCA. Internal left primer: GGAAACGCATGAAGAGAAGG. Internal right primer: CCTTGCTAGCGGATAAGCAG. Internal WT amplicon: 1196 bp. Deletion size: 647 bp. Deletion left flank: ACTGTTATATCAAATATGGAAACTGATTTG. Deletion right flank: AAGATTTAGTTGATGAAGAAGATAGAGGAG.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037125 Copy
http://www.wormbase.org/db/get?name=WBStrain00037126
Source Database: WormBase (WB)
Affected Genes: WBGene00013147(eyg-1)
Genomic Alteration: WBGene00013147(eyg-1)
Availability: available
Source References: EMPTY
Synonyms: Y53C12C.1(ok2201) II.
Alternate IDs: WB-STRAIN:VC2162, CGC_VC2162
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y53C12C.1. External left primer: GAACTTCGAAAATGGGCAAA. External right primer: TGATGCCGCCATATATTCAA. Internal left primer: ATCAATCAAGAATGCCCACC. Internal right primer: TTCACTCACGGTTTACCACG. Internal WT amplicon: 2128 bp. Deletion size: 1210 bp. Deletion left flank: AAAAAGTTTGAGTAGACTTTAACTGAGTAT. Deletion right flank: TTAATATAATTCAATTAATTCATCAGGTAA. Insertion Sequence: A."
Proper citation: RRID:WB-STRAIN:WBStrain00037126 Copy
http://www.wormbase.org/db/get?name=WBStrain00037123
Source Database: WormBase (WB)
Affected Genes: WBGene00003998(pgp-4)
Genomic Alteration: WBGene00003998(pgp-4)
Availability: available
Source References: EMPTY
Synonyms: pgp-4(gk1006) X.
Alternate IDs: WB-STRAIN:VC2159, CGC_VC2159
Notes: F42E11.1. External left primer: TGGACTTGCATGGAACACAT. External right primer: AATCATTTCTTCACGGGCAC. Internal left primer: ACACTACAACTTACCCGCCG. Internal right primer: GGTGGTGTCATCTTTGGCTT. Internal WT amplicon: 2647 bp. Deletion size: 1230 bp. Deletion left flank: ACCACAAAATAATGTTTTCATTTTTAACTT. Deletion right flank: CTTATCCAACTAGACCTGACGTCAAAATTT. Insertion Sequence: TCAATTT.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037123 Copy
http://www.wormbase.org/db/get?name=WBStrain00037132
Source Database: WormBase (WB)
Affected Genes: WBGene00013969(tep-1)
Genomic Alteration: WBGene00013969(tep-1)
Availability: available
Source References: EMPTY
Synonyms: ZK337.1(ok2874) I.
Alternate IDs: WB-STRAIN:VC2168, CGC_VC2168
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK337.1. External left primer: TCCAGACGTGCTGACAACTC. External right primer: GGCGCTACTCCACCATTAAA. Internal left primer: TCAGCTTTTGGCGATAGTGAT. Internal right primer: GTCTTCCATGGCTGTGAGGT. Internal WT amplicon: 1142 bp. Deletion size: 360 bp. Deletion left flank: TCTCTCTCAATTATTCGTTGGTTAGTATCC. Deletion right flank: AAGTGGGCTTTTTTCAAATTTTTTCAAGTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037132 Copy
http://www.wormbase.org/db/get?name=WBStrain00037130
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00019401(nuo-4)
Genomic Alteration: WBGene00000254(bli-4), WBGene00019401(nuo-4)
Availability: available
Source References: EMPTY
Synonyms: nuo-4(ok2483) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2166, CGC_VC2166
Notes: K04G7.4. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2483 homozygotes (early- to mid-larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AAAACCCAAACGTGGCAATA. External right primer: TTGTTAAGACCATCATGCCG. Internal left primer: AAAAGTGTGCGTGGGGTAAT. Internal right primer: GTTCCATGAGCAAATTGGGA. Internal WT amplicon: 3154 bp. Deletion size: 1295 bp. Deletion left flank: TCTATTACTTAAAGCAAATTTTCAAATTGA. Deletion right flank: AGTCTAGAAACAATTATTTTGAAAGAAAAT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037130 Copy
http://www.wormbase.org/db/get?name=WBStrain00037137
Source Database: WormBase (WB)
Affected Genes: WBGene00012629(slc-36.3)
Genomic Alteration: WBGene00012629(slc-36.3)
Availability: available
Source References: EMPTY
Synonyms: Y38H6C.17(ok2911) V.
Alternate IDs: WB-STRAIN:VC2174, CGC_VC2174
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y38H6C.17. External left primer: CCGGTTGCTTACATGCCTAC. External right primer: GATTCGCCAATCTTCCAAAA. Internal left primer: AAGCAATACGTACCGGTCTACA. Internal right primer: AAAGTTTCCAAATTTTTCGGC. Internal WT amplicon: 1372 bp. Deletion size: 525 bp. Deletion left flank: ATATACCCAAAGAATCCTAGAAATGCATAA. Deletion right flank: CGTTGCCGAAAAATTTGGAAACTTTCTATA."
Proper citation: RRID:WB-STRAIN:WBStrain00037137 Copy
http://www.wormbase.org/db/get?name=WBStrain00037135
Source Database: WormBase (WB)
Affected Genes: WBGene00006576(tkr-1)
Genomic Alteration: WBGene00006576(tkr-1)
Availability: available
Source References: EMPTY
Synonyms: tkr-1(ok2886) III.
Alternate IDs: WB-STRAIN:VC2171, CGC_VC2171
Notes: C38C10.1. External left primer: TGGTCATGGTCGAATCCATA. External right primer: ACATTTTCCAATTCTTGCGG. Internal left primer: CTGCAATTGAATCGGAAACA. Internal right primer: TTTTGTTGCTCCGGATTTTC. Internal WT amplicon: 1214 bp. Deletion size: 755 bp. Deletion left flank: TACTATGACTGGTGGTATGGTGATCTATGT. Deletion right flank: ACTATCAAAAAATCAATGAAAATCCGGAGC. Insertion Sequence: GGTGATCTATGT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037135 Copy
http://www.wormbase.org/db/get?name=WBStrain00037143
Source Database: WormBase (WB)
Affected Genes: WBGene00012473(Y17G7B.22)
Genomic Alteration: WBGene00012473(Y17G7B.22)
Availability: available
Source References: EMPTY
Synonyms: Y17G7B.22(gk1010) II.
Alternate IDs: WB-STRAIN:VC2180, CGC_VC2180
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y17G7B.22. External left primer: CCCGTAGTTCATCGATTGCT. External right primer: AAAAAGAATACCACCGGCCT. Internal left primer: ATCTGTTGCCTTCTGTTGGG. Internal right primer: TCGCAGGAGTTTGGGTACTT. Internal WT amplicon: 2119 bp. Deletion size: 1350 bp. Deletion left flank: AATGGACCTGAAAGATTGAAAACAATTGAC. Deletion right flank: TTTTGGTGCTTCAAAAAACATCAAAAAATA. Insertion Sequence: GTGTGGTAAGCGAAAGTAAGCGAAAATTCAAGCTTCGATTGAATTATCATTTCAAAAAG AAATAAATGGAAAACGTATTGTAATCGCTGAACAAACTCCAAAAAATTTGATACTTTTT GATGTT."|"Y17G7B.22. External left primer: CCCGTAGTTCATCGATTGCT. External right primer: AAAAAGAATACCACCGGCCT. Internal left primer: ATCTGTTGCCTTCTGTTGGG. Internal right primer: TCGCAGGAGTTTGGGTACTT. Internal WT amplicon: 2119 bp. Deletion size: 1350 bp. Deletion left flank: AATGGACCTGAAAGATTGAAAACAATTGAC. Deletion right flank: TTTTGGTGCTTCAAAAAACATCAAAAAATA. Insertion Sequence: GTGTGGTAAGCGAAAGTAAGCGAAAATTCAAGCTTCGATTGAATTATCATTTCAAAAAGAAATAAATGGAAAACGTATTGTAATCGCTGAACAAACTCCAAAAAATTTGATACTTTTTGATGTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037143 Copy
http://www.wormbase.org/db/get?name=WBStrain00037144
Source Database: WormBase (WB)
Affected Genes: WBGene00000108(alh-2)|WBGene00016918(test-1)
Genomic Alteration: WBGene00000108(alh-2), WBGene00016918(test-1)
Availability: available
Source References: EMPTY
Synonyms: C54E4.2(gk1083) IV; alh-2(gk3053) V.
Alternate IDs: WB-STRAIN:VC2181, CGC_VC2181
Notes: K04F1.15, C54E4.2. The allele gk1083 was identified by PCR, validated by CGH, and can be detected using the following PCR primers. External left primer: TTTTTGACGACCAACCAACA. External right primer: CGAGGCTCTTTACGCAATTC. Internal left primer: CGCAGCGAACAAAGTTATGA. Internal right primer: CGTGGCGAGACCTATAAAGC. Internal WT amplicon: 1288 bp. Deletion size: 575 bp. Deletion left flank: TGAATACCGTTAATTTTTTTTTTTTAATTA. Deletion right flank: TTCGCTGAAAAATATAATTTCTTTCTGGTG. The allele gk3053 was identified by CGH and not confirmed by PCR. Left flanking probe: ATTTTACATTAGTCCGTGAATTTCAGATACTACGCCGGATATGCTGATAA. Right flanking probe: GCGCGGGATCAGTTTGGGTCAACTGTTATGATGTTTTTGATCCTGCTGCT. Left deleted probe: TATGCTGATAAAAACCACGGAAAAACCATTCCCGTCGGTGGAGACTATTT. Right deleted probe: TGAATAAAGCTCTTCAAGTCGCAAATACTATCCGCGCGGGATCAGTTTGG.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037144 Copy
http://www.wormbase.org/db/get?name=WBStrain00037142
Source Database: WormBase (WB)
Affected Genes: WBGene00020630(T20F7.1)
Genomic Alteration: WBGene00020630(T20F7.1)
Availability: available
Source References: EMPTY
Synonyms: T20F7.1(gk998) X.
Alternate IDs: WB-STRAIN:VC2179, CGC_VC2179
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"T20F7.1. External left primer: AAACGACACTCCGTTTGGAC. External right primer: AGTGACCGGAAGCTCTTGAA. Internal left primer: TCCGGGAGAAGTATTCATGG. Internal right primer: CGGTCCTCCACATCTGAAAT. Internal WT amplicon: 2098 bp. Deletion size: 305 bp. Deletion left flank: ATTTTTTAAATGAGTTTGATATAAAAATGA. Deletion right flank: TATGAATCAACAGTATGGTGTGGAAAACCA."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037142 Copy
http://www.wormbase.org/db/get?name=WBStrain00037224
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00000381(cct-6)
Genomic Alteration: WBGene00000254(bli-4), WBGene00000381(cct-6)
Availability: available
Source References: EMPTY
Synonyms: cct-6(ok2904) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2279, CGC_VC2279
Notes: F01F1.8. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2904 homozygotes (early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AAAGTTGGGCTTGTTGAACG. External right primer: CCCTCGAGTTGCTTGAAAAG. Internal left primer: ATTCTGGTTGTGGCTGCTTC. Internal right primer: GCGTGACCTCCTTGTAGAGG. Internal WT amplicon: 1286 bp. Deletion size: 605 bp. Deletion left flank: CCGAGCTTGGCACGTCCCTTCAGGTTCTCA. Deletion right flank: CTTCAGTCTTCTCGTATTCCAAAGAAACGT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037224 Copy
http://www.wormbase.org/db/get?name=WBStrain00037225
Source Database: WormBase (WB)
Affected Genes: WBGene00001089(dre-1)
Genomic Alteration: WBGene00001089(dre-1)
Availability: available
Source References: EMPTY
Synonyms: dre-1(ok2905) V/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2280, CGC_VC2280
Notes: K04A8.6. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2905 homozygotes (early larval arrest; escapers may lay eggs that hatch). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGAAGTAGGGATTCGCATGA. External right primer: CCCCTTTCAATTTCGAGTCA. Internal left primer: CAGCCAAAGTATTTCGAGCA. Internal right primer: CAGAAGGTCGAGGGAGACAT. Internal WT amplicon: 1211 bp. Deletion size: 741 bp. Deletion left flank: CTGCTGCCTGCACACTGGATCAGCCCGAAA. Deletion right flank: TTTTTTTCATTATTTCTTATCTCAAAAATG. Insertion Sequence: TCATTTTTTTATCTATCAT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037225 Copy
http://www.wormbase.org/db/get?name=WBStrain00037228
Source Database: WormBase (WB)
Affected Genes: WBGene00010329(pcdr-1)
Genomic Alteration: WBGene00010329(pcdr-1)
Availability: available
Source References: EMPTY
Synonyms: F59D12.1(gk1122) X.
Alternate IDs: WB-STRAIN:VC2285, CGC_VC2285
Notes: F59D12.1. Identified by PCR, validated by CGH. External left primer: CTCACAAAAAGGGGCGAATA. External right primer: TACCCCTTACACTAACGGCG. Internal left primer: GGTTGTGTTCTATCCCGACG. Internal right primer: ATGAGTGCTTGGGACTTTGG. Internal WT amplicon: 937 bp. Deletion size: 585 bp. Deletion left flank: ACTAGGTTTTCTGTTTGTCACATTTTTCTT. Deletion right flank: CTTATTTGAATATAAACATTCAAGATTTCT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037228 Copy
http://www.wormbase.org/db/get?name=WBStrain00037229
Source Database: WormBase (WB)
Affected Genes: WBGene00002179(jph-1)
Genomic Alteration: WBGene00002179(jph-1)
Availability: available
Source References: PMID:39381635
Synonyms: jph-1(ok2823) I.
Alternate IDs: WB-STRAIN:VC2286, CGC_VC2286
Notes: T22C1.7. External left primer: TGGAATGTGTGGTTGAAGGA. External right primer: GGTGATCCCTCTGGCTGTAA. Internal left primer: TTGTGAATTGATTGGTGTTTGA. Internal right primer: GGCCTTTCTGGTAGAGGAGG. Internal WT amplicon: 1144 bp. Deletion size: 637 bp. Deletion left flank: TTCGGCATCACATGATTGTGATACGCTTTT. Deletion right flank: AATTTCAAAAATTTCCCTCATAATTTCAAA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037229 Copy
http://www.wormbase.org/db/get?name=WBStrain00037226
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00011631(tgs-1)
Genomic Alteration: WBGene00000254(bli-4), WBGene00011631(tgs-1)
Availability: available
Source References: EMPTY
Synonyms: T08G11.4(ok2909) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2281, CGC_VC2281
Notes: T08G11.4. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2909 homozygotes (early to mid-larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CGATTTCCATGCGACTTTTT. External right primer: GAAATTTGTCGCAAATCGGT. Internal left primer: CTCGACGAGCTGAAAAATGT. Internal right primer: ACGGAGTCGCTTCTTTTCTG. Internal WT amplicon: 1281 bp. Deletion size: 505 bp. Deletion left flank: CAAATGAGACTAATGATGTTCTAAGTATAT. Deletion right flank: CAGTGAATGCATCGACAACAACAGAAACAT. Insertion Sequence: AT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037226 Copy
http://www.wormbase.org/db/get?name=WBStrain00037231
Source Database: WormBase (WB)
Affected Genes: WBGene00006829(unc-101)|WBGene00012354(cox-4)
Genomic Alteration: WBGene00006829(unc-101), WBGene00012354(cox-4)
Availability: available
Source References: EMPTY
Synonyms: W09C5.8(ok2908)/hIn1 [unc-101(sy241)] I.
Alternate IDs: WB-STRAIN:VC2290, CGC_VC2290
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"W09C5.8. Apparent homozygous lethal deletion chromosome balanced by unc-101-marked inversion. Heterozygotes are WT, and segregate WT, Unc-101 hIn1 homozygotes, and ok2908 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TTCCTCGCTACTACCCGCTA. External right primer: CCCGTGTGTTCTGTTGTTTG. Internal left primer: CAGCCCATCTCTCAAGAAGC. Internal right primer: TCCTCTTCCACGTTTCCATC. Internal WT amplicon: 1106 bp. Deletion size: 565 bp. Deletion left flank: CCTTCTCGAGCACAAGCGCACGCTCAACCT. Deletion right flank: AATAAATAACTGGTTTATGGGTTGAAAATG. Insertion Sequence: CAAC."
Proper citation: RRID:WB-STRAIN:WBStrain00037231 Copy
http://www.wormbase.org/db/get?name=WBStrain00037232
Source Database: WormBase (WB)
Affected Genes: WBGene00000209(asg-1)|WBGene00000254(bli-4)
Genomic Alteration: WBGene00000209(asg-1), WBGene00000254(bli-4)
Availability: available
Source References: EMPTY
Synonyms: asg-1(ok2950) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2291, CGC_VC2291
Notes: K07A12.3. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2950 homozygotes (sterile, lays no eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TGGCAGTTCTCTCGCTTTTT. External right primer: ATAAGCCAGGATGATGCGAC. Internal left primer: GTAATCCGGATCTTGCTTGG. Internal right primer: ACACTCGAGAGGCTGGAGAA. Internal WT amplicon: 1204 bp. Deletion size: approximately 1203 bp.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037232 Copy
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