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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037051
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017296(F09E10.6)
Genomic Alteration: WBGene00017296(F09E10.6)
Availability: available
References:
Synonyms: F09E10.6(ok2817) X.
Alternate IDs: WB-STRAIN:VC2048, CGC_VC2048
Notes: F09E10.6. External left primer: GCCACCTGCCGAGTTATTTA. External right primer: CAATTTCCTGCCATTCCTGT. Internal left primer: CGCCATGAGGTGTTTACTGA. Internal right primer: GCTACTCCCCCACCAAAAGT. Internal WT amplicon: 1115 bp. Deletion size: 635 bp. Deletion left flank: GCAGAACCCGATAGATGTCGGGCCATAGTA. Deletion right flank: AGTTTTCAGGGCCTGTTGCCTGCCTACTTC. Insertion Sequence: ACAA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037051 Copy
http://www.wormbase.org/db/get?name=WBStrain00037052
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00012974(Y48A6C.1)
Genomic Alteration: WBGene00012974(Y48A6C.1)
Availability: available
References:
Synonyms: Y48A6C.1(gk955) III.
Alternate IDs: WB-STRAIN:VC2049, CGC_VC2049
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y48A6C.1. External left primer: GGGTTTTCAGCCATTTTTCA. External right primer: AATTTCAATCAGAAACGCGG. Internal left primer: TTGTATCGATTAATCCCGGC. Internal right primer: TTTCGTCCGAACCGTTAGTC. Internal WT amplicon: 2443 bp. Deletion size: 1549 bp. Deletion left flank: CCATTTTTCAGCAAAAATGCACTGACTCTG. Deletion right flank: CGTAAATTTTTTCGGGTTTTTAAACTCCAA."
Proper citation: RRID:WB-STRAIN:WBStrain00037052 Copy
http://www.wormbase.org/db/get?name=WBStrain00037050
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017296(F09E10.6)
Genomic Alteration: WBGene00017296(F09E10.6)
Availability: available
References:
Synonyms: F09E10.6(ok2816) X.
Alternate IDs: WB-STRAIN:VC2047, CGC_VC2047
Notes: F09E10.6. External left primer: GCCACCTGCCGAGTTATTTA. External right primer: CAATTTCCTGCCATTCCTGT. Internal left primer: CGCCATGAGGTGTTTACTGA. Internal right primer: GCTACTCCCCCACCAAAAGT. Internal WT amplicon: 1115 bp. Deletion size: 398 bp. Deletion left flank: GAGGTTATTGAAAAAAAAATAAAGCAACAA. Deletion right flank: GCTTGGTGTTAACACCACATAGTGCGAAAG.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037050 Copy
http://www.wormbase.org/db/get?name=WBStrain00037055
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00013796(Y116A8C.19)
Genomic Alteration: WBGene00013796(Y116A8C.19)
Availability: available
References:
Synonyms: Y116A8C.19(gk958) IV.
Alternate IDs: WB-STRAIN:VC2052, CGC_VC2052
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y116A8C.19. External left primer: GAAAAGCTCAATTTTTGCCG. External right primer: TCGCCTTCTTTTTACAGCGT. Internal left primer: CGACGTGCTATCGAACTTGA. Internal right primer: CTCCGGAATCTAGCAACCAA. Internal WT amplicon: 957 bp. Deletion size: 210 bp. Deletion left flank: CAAAGAACTGTTTTATAGTTACGATGAGTT. Deletion right flank: GAAAACTGATCTCCGTCATAAGATCCTGGA."
Proper citation: RRID:WB-STRAIN:WBStrain00037055 Copy
http://www.wormbase.org/db/get?name=WBStrain00037056
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000254(bli-4)|WBGene00020094(wip-1)
Genomic Alteration: WBGene00000254(bli-4), WBGene00020094(wip-1)
Availability: available
References:
Synonyms: wip-1(ok2435) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2053, CGC_VC2053
Notes: R144.4. Homozygous sterile or near-sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2435 homozygotes (grotty, Unc, with vulval blip). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AACGTATTCCGAAGTGCGAC. External right primer: CCATGAAGAAACCCAGGAAA. Internal left primer: TCAGAAAGATTGTTCCGGTTTT. Internal right primer: GGGGGATTGACGGACTATTT. Internal WT amplicon: 3053 bp. Deletion size: 1544 bp. Deletion left flank: AAATAAGACGGTAAAGAATTTTATCAGAAT. Deletion right flank: TCAGTTCCAAGCTCAAAACCGACTCCACCT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037056 Copy
http://www.wormbase.org/db/get?name=WBStrain00037053
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00011626(T08G5.7)
Genomic Alteration: WBGene00011626(T08G5.7)
Availability: available
References:
Synonyms: T08G5.7(gk956) V.
Alternate IDs: WB-STRAIN:VC2050, CGC_VC2050
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"T08G5.7. External left primer: CATCGCCTCAATCAGTCAAA. External right primer: TGTTGCCCCCTAATTTGTTG. Internal left primer: TTTCTTGCCTCCCTCTTGAA. Internal right primer: CAGTTTCCGTTTCGAAGCTC. Internal WT amplicon: 1279 bp. Deletion size: 581 bp. Deletion left flank: CAATCGTGTCACCTTATCATTCACATTTCT. Deletion right flank: GGCTGAAGTTGATCAATTCCGAATTCAGAG."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037053 Copy
http://www.wormbase.org/db/get?name=WBStrain00037054
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018539(nhr-185)
Genomic Alteration: WBGene00018539(nhr-185)
Availability: available
References:
Synonyms: nhr-185(gk957) V.
Alternate IDs: WB-STRAIN:VC2051, CGC_VC2051
Notes: F47C10.1. External left primer: TCATTCTGGCAGGAAATTCA. External right primer: GGCGTAACGAAGTCCGATAA. Internal left primer: TCCGGTTAGTCCTGCAATTC. Internal right primer: CTGCTACCCATGTCGAGTGA. Internal WT amplicon: 2231 bp. Deletion size: 847 bp. Deletion left flank: AGCTGAGCCCGGTAGATGTCGGACCACTAA. Deletion right flank: GAGGTATAAATAAAAGTCTATAAGAAAGAC.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037054 Copy
http://www.wormbase.org/db/get?name=WBStrain00037059
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00016620(dhhc-9)
Genomic Alteration: WBGene00016620(dhhc-9)
Availability: available
References:
Synonyms: C43H6.7(gk985) X.
Alternate IDs: WB-STRAIN:VC2067, CGC_VC2067
Notes: C43H6.7. External left primer: ATCTTCATATTTGACCGGCG. External right primer: TCCATTCTGCTTCGTCACTG. Internal left primer: ACCATCACCAGAAGAATGCC. Internal right primer: GGTCAAAGCTGCGAACTCAT. Internal WT amplicon: 2524 bp. Deletion size: 438 bp. Deletion left flank: GCTTTGTAGTAATGATATTGGATGTTGAAT. Deletion right flank: TTTTCGTAATTACTACAGTGTTCTCAAAAT.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037059 Copy
http://www.wormbase.org/db/get?name=WBStrain00037058
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00012473(Y17G7B.22)
Genomic Alteration: WBGene00012473(Y17G7B.22)
Availability: available
References:
Synonyms: Y17G7B.22(gk1012) II.
Alternate IDs: WB-STRAIN:VC2063, CGC_VC2063
Notes: Made_by: Vancouver KO Group|"Mutagen: Formaldehyde"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y17G7B.22. External left primer: CCCGTAGTTCATCGATTGCT. External right primer: AAAAAGAATACCACCGGCCT. Internal left primer: ATCTGTTGCCTTCTGTTGGG. Internal right primer: TCGCAGGAGTTTGGGTACTT. Internal WT amplicon: 2119 bp. Deletion size: 1795 bp. Deletion left flank: AAAGACGAAATTGAGAAGAAAATTGCTGAG. Deletion right flank: TTTTGGTGCTTCAAAAAACATCAAAAAATA. Insertion Sequence: AAAATTTGATACTTTTTGATGTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037058 Copy
http://www.wormbase.org/db/get?name=WBStrain00037062
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00009553(hinf-1)
Genomic Alteration: WBGene00009553(hinf-1)
Availability: available
References:
Synonyms: F39B2.1(gk3174) I.
Alternate IDs: WB-STRAIN:VC2071, CGC_VC2071
Notes: Made_by: Vancouver KO Group|"This strain is homozygous for a deletion (gk3174) in F39B2.1, detectable by PCR using the following primers. External left primer: CCGGTAGTAGCTTTCCCCTC. External right primer: AAGTCGCATAAGTCCATCGG. Internal left primer: ATATCAACCATCCAGCCAGC. Internal right primer: CGTCAGAATGGTACACAGCG. Internal WT amplicon: 2358 bp. Deletion size: approximately 450 bp. Validation: gk3174 passed by CGH. Left deleted probe: CATGGTCGCGACGAGGCTCAATCTGATCCATCACGCCAACTTTTGTTTAA. Right deleted probe: GATAGAATTCAACAGAATTTTTCGAGTGAGTAAGGATTTCTGGACAGTGA."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037062 Copy
http://www.wormbase.org/db/get?name=WBStrain00037063
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001707(grh-1)
Genomic Alteration: WBGene00001707(grh-1)
Availability: available
References:
Synonyms: grh-1(gk960) I.
Alternate IDs: WB-STRAIN:VC2072, CGC_VC2072
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y48G8AR.1. External left primer: GTTTTCCTAAAGTTCGGCCC. External right primer: CCCTTTCACTTTTCACCGAA. Internal left primer: CATAAGCTCGATCCCAAAGC. Internal right primer: CTTTGAATCGCGGAATTTGT. Internal WT amplicon: 3012 bp. Deletion size: 786 bp. Deletion left flank: CAATGTCGATTGGCTCGTTTTTGAATTCTA. Deletion right flank: AATAGTTAAAGTCCAATTCATTGTTTATTA."
Proper citation: RRID:WB-STRAIN:WBStrain00037063 Copy
http://www.wormbase.org/db/get?name=WBStrain00037061
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00013797(Y116A8C.20)
Genomic Alteration: WBGene00013797(Y116A8C.20)
Availability: available
References:
Synonyms: Y116A8C.20(gk913) IV.
Alternate IDs: WB-STRAIN:VC2069, CGC_VC2069
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y116A8C.20. External left primer: ACGCTGTAAAAAGAAGGCGA. External right primer: TGAGCACGTTTTTGAAATGC. Internal left primer: AGCGGCTGAAGAGAAGTTTG. Internal right primer: GACTGACGCAGTGACAGGAA. Internal WT amplicon: 1414 bp. Deletion size: 378 bp. Deletion left flank: ATGGGCGGAGCTTCCCGATCAATAATTGAC. Deletion right flank: CAATTACCCTCCATCCCTTTCACATACATC."
Proper citation: RRID:WB-STRAIN:WBStrain00037061 Copy
http://www.wormbase.org/db/get?name=WBStrain00037066
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020827(T26A8.4)
Genomic Alteration: WBGene00020827(T26A8.4)
Availability: available
References:
Synonyms: T26A8.4(gk917) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2081, CGC_VC2081
Notes: T26A8.4. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk917 homozygotes (Dpyish, late larval arrest or sterile adult). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TGCTTTGGCTCTTCTTGGAT. External right primer: TGTTTGCGCTGAGAGAGAGA. Internal left primer: GCTGAACTAATCCAGGCTGC. Internal right primer: TCCAACGTTCAAGATTCCAA. Internal WT amplicon: 1977 bp. Deletion size: 722 bp. Deletion left flank: TAATTATTATGGAAAAGTGATTTCGATTTT. Deletion right flank: AATTATTCCCATTTATTAATGCGTCAATAA. Insertion Sequence: TTGCTTACCTCCAGGGAGG.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037066 Copy
http://www.wormbase.org/db/get?name=WBStrain00037067
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00015447(srab-2)
Genomic Alteration: WBGene00015447(srab-2)
Availability: available
References:
Synonyms: srab-2(gk686) V/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2082, CGC_VC2082
Notes: C04F5.5. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk686 homozygotes (embryonic or early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AGTCAGACCCGCTTTGAGAA. External right primer: CAAACATGGTGCAAGACCAG. Internal left primer: CGAAGAATGCTTGCAAATGA. Internal right primer: TCCTTCGAGCCAGCTGTATT. Internal WT amplicon: 2299 bp. Deletion size: 1150 bp. Deletion left flank: AAGAAACTCTTTTGAGTTACCTCATTTTTT. Deletion right flank: TTTCTCCACGCTACTCCGACACATTATCAC.|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037067 Copy
http://www.wormbase.org/db/get?name=WBStrain00037065
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00016534(C39D10.7)
Genomic Alteration: WBGene00016534(C39D10.7)
Availability: available
References:
Synonyms: C39D10.7(ok2758) X.
Alternate IDs: WB-STRAIN:VC2074, CGC_VC2074
Notes: C39D10.7. External left primer: TTTGCATGTATCCACGGTGT. External right primer: TAAGCAGCGGAAACCATTTT. Internal left primer: GGGGCACATGAGGAAATAAG. Internal right primer: TTTCAAACAAAAATTCCCCC. Internal WT amplicon: 1179 bp. Deletion size: 691 bp. Deletion left flank: CAGATAACTTGTGATTTTGCACAGTCTAAC. Deletion right flank: TAGAGTTGTTGTGAAGATTGTTGATGTGTA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037065 Copy
http://www.wormbase.org/db/get?name=WBStrain00037024
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003151(mca-1)
Genomic Alteration: WBGene00003151(mca-1)
Availability: available
References:
Synonyms: mca-1(ok2532) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2000, CGC_VC2000
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"W09C2.3. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2532 homozygotes (early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AGGTTAGAAGCTGACGAGCG. External right primer: TGAATCCGATCCAGTTCTCC. Internal left primer: CAGTCGGCAGATTTCACAGA. Internal right primer: CCGGAAAAATGCTCATCACT. Internal WT amplicon: 3166 bp. Deletion size: 1418 bp. Deletion left flank: TCGCGGATTCTCTCATTGAATTCCTTTCCT. Deletion right flank: ACTTTTCCATTTTCGTCGCGGATTCTCTCA."
Proper citation: RRID:WB-STRAIN:WBStrain00037024 Copy
http://www.wormbase.org/db/get?name=WBStrain00037028
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00010704(K09A11.1)
Genomic Alteration: WBGene00010704(K09A11.1)
Availability: available
References:
Synonyms: K09A11.1(gk1064) X.
Alternate IDs: WB-STRAIN:VC2006, CGC_VC2006
Notes: K09A11.1. Identified by PCR, validated by CGH. External left primer: GAGCAACGAAATTTTGGGAA. External right primer: GTTATGTTTGCCGCGAGATT. Internal left primer: GGAGTATCCGTCCGCAATAG. Internal right primer: TGCAGCTCTCTTTCCATGTG. Internal WT amplicon: 2161 bp. Deletion size: 810 bp. Deletion left flank: TTTGTTCTCCACTCTTTATTCGTATTGAAT. Deletion right flank: GTACGAAGACCAACTAAGAATGGAATTGAA. Insertion Sequence: CTTATTTC.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037028 Copy
http://www.wormbase.org/db/get?name=WBStrain00037034
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001461(flp-18)
Genomic Alteration: WBGene00001461(flp-18)
Availability: available
References:
Synonyms: flp-18(gk3063) X.
Alternate IDs: WB-STRAIN:VC2016, CGC_VC2016
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y48D7A.2. External left primer: TGTGCCACTCACCGATGACAC. External right primer: CATCATCATGGCGCTACG. Internal left primer: GTCCTATCAGTACCTCATGGG. Internal right primer: CGAATACCTTGTACACGC. Internal WT amplicon: 2980 bp. Deletion size: 1312 bp. Deletion left flank: AACACACGTCAACCATGAACAAATCTGCTT. Deletion right flank: AAATTTCAAATTCATGCTTTCAAATCGACA."
Proper citation: RRID:WB-STRAIN:WBStrain00037034 Copy
http://www.wormbase.org/db/get?name=WBStrain00037031
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006829(unc-101)|WBGene00013020(ndrr-1)
Genomic Alteration: WBGene00006829(unc-101), WBGene00013020(ndrr-1)
Availability: available
References:
Synonyms: Y48G10A.3(ok2508)/hIn1 [unc-101(sy241)] I.
Alternate IDs: WB-STRAIN:VC2011, CGC_VC2011
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y48G10A.3. Apparent homozygous lethal deletion chromosome balanced by unc-101-marked inversion. Heterozygotes are WT, and segregate WT, Unc-101 hIn1 homozygotes, and ok2508 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: GTGGATGGTTTTCGCAGTTT. External right primer: TGACATGCAGCCTCTAATGG. Internal left primer: ATTCTGCGTCTCCTGCATCT. Internal right primer: AAAAGTGAACACGGCCTTTG. Internal WT amplicon: 2246 bp. Deletion size: 1628 bp. Deletion left flank: AAAAGAGCATCATGCTCTCCGTCACAGCGT. Deletion right flank: TTTTAAAAAAGTTTTGGTTTTTTTTTTAAA."
Proper citation: RRID:WB-STRAIN:WBStrain00037031 Copy
http://www.wormbase.org/db/get?name=WBStrain00037032
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00012473(Y17G7B.22)|WBGene00016114(flp-27)
Genomic Alteration: WBGene00012473(Y17G7B.22), WBGene00016114(flp-27)
Availability: available
References:
Synonyms: flp-27(gk3331) Y17G7B.22(gk1062) II; gkDf45 X.
Alternate IDs: WB-STRAIN:VC2012, CGC_VC2012
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk1062) in Y17G7B.22, detectable by PCR using the following primers. External left primer: CCCGTAGTTCATCGATTGCT. External right primer: AAAAAGAATACCACCGGCCT. Internal left primer: ATCTGTTGCCTTCTGTTGGG. Internal right primer: TCGCAGGAGTTTGGGTACTT. Internal WT amplicon: 2119 bp. Deletion size: 1412 bp. Deletion left flank: AAATAGACTATTTCGGAAAATGGAAATGAG. Deletion right flank: AAAATTATTGATTTTGACCCCAAAAATTTA. Insertion Sequence: TTGT. Validation: gk1062 passed by diagnostic PCR and CGH. Other deletions (gk3331, gkDf45) identified by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037032 Copy
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