Searching the RRID Resource Information Network
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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037333
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017803(F26A1.4)
Genomic Alteration: WBGene00017803(F26A1.4)
Availability: available
References:
Synonyms: F26A1.4(gk1167) III.
Alternate IDs: WB-STRAIN:VC2441, CGC_VC2441
Notes: F26A1.4. External left primer: TTTAGGTCTGGCACTACCCG. External right primer: AAAACATTGACACACCTGCG. Internal left primer: AAAGCGGCAGCAGTTAAGAA. Internal right primer: CTACCGGTACTGCCATTCGT. Internal WT amplicon: 1327 bp. Deletion size: 174 bp. Deletion left flank: TTATGTTTATGTTTCAGTTCTGACACGCCA. Deletion right flank: TTAAAATATTTCAGATTGAATGTGGAGATG.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037333 Copy
http://www.wormbase.org/db/get?name=WBStrain00037338
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001542(gcy-17)
Genomic Alteration: WBGene00001542(gcy-17)
Availability: available
References:
Synonyms: gcy-17(gk1155) I.
Alternate IDs: WB-STRAIN:VC2450, CGC_VC2450
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"W03F11.2. External left primer: TCTAGGTCAAAAGCGGAGGA. External right primer: CTTTAACACGGTGAAGGGGA. Internal left primer: GGAAATGGAGCATCGAGGTA. Internal right primer: CATATGCAAGATGTTTGCCG. Internal WT amplicon: 1241 bp. Deletion size: 655 bp. Deletion left flank: GCATTGAGCTTCTGCTAATGACATCGGCCA. Deletion right flank: TAAATTTTGAGAGTAAAGTTCTTACATTTC."
Proper citation: RRID:WB-STRAIN:WBStrain00037338 Copy
http://www.wormbase.org/db/get?name=WBStrain00037336
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00019205(kcc-2)
Genomic Alteration: WBGene00019205(kcc-2)
Availability: available
References:
Synonyms: kcc-2(ok3074) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2448, CGC_VC2448
Notes: H16O14.1. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3074 homozygotes (sterile with no eggs, often with vulval blip). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGACGGCCAGATTCAGTAAA. External right primer: CGTAATGGCGGTTTTTGATT. Internal left primer: TTTGGTAACTTCTGGCCGTC. Internal right primer: GGGGCTTGTTTGAAAGAACA. Internal WT amplicon: 1227 bp. Deletion size: 582 bp. Deletion left flank: CAAACTAAGTATTTATTCTTTTAACATTTT. Deletion right flank: AGTAATTCTTTTTGGATGTTTCATGTCAAC. Insertion Sequence: TAAAAAGTATTTATTCTTTTAACATTCTTTTAACAC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037336 Copy
http://www.wormbase.org/db/get?name=WBStrain00037308
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00012888(sas-6)
Genomic Alteration: WBGene00012888(sas-6)
Availability: available
References:
Synonyms: sas-6(ok2554) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2399, CGC_VC2399
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y45F10D.9. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2554 homozygotes (sterile, no eggs). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTCATGAGAATCCCCGTTGT. External right primer: ACGGGATAAGTGGCTCACAC. Internal left primer: CGGAGGACTCCCAACTGATA. Internal right primer: TGAAAATGCGGGAAACTCTC. Internal WT amplicon: 2129 bp. Deletion size: 1435 bp. Deletion left flank: TATTGTCACGGAATGGGGTGCGCTGAAATT. Deletion right flank: CTGTTACTTTTGAAAATCGTTTGCTCCCTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037308 Copy
http://www.wormbase.org/db/get?name=WBStrain00037302
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001545(gcy-20)|WBGene00005692(sru-29)
Genomic Alteration: WBGene00001545(gcy-20), WBGene00005692(sru-29)
Availability: available
References:
Synonyms: gkDf17 II; gkDf18 C50C10.2(gk3035) gcy-20(gk1184) V.
Alternate IDs: WB-STRAIN:VC2391, CGC_VC2391
Notes: C40A11.7, C40A11.8, C40A11.1, F56E10.1, Y38C9A.1, C50C10.2, F21H7.9. The allele gk1184 was identified by PCR, validated by CGH, and can be detected using the following PCR primers. External left primer: AATCACTTTCGGTGCAGCTT. External right primer: GTATGCCCCACAGTTTTGCT. Internal left primer: AGTATCGCGGCATTGTTAGC. Internal right primer: TGCTCAAGCTTGGAGAGACA. Internal WT amplicon: 2419 bp. Deletion size: 2042 bp. Deletion left flank: ACCGCAATTAATTCCAATTCTAAGGTTTAT. Deletion right flank: ACTGGCGTCTTACAGTAAATTTTGTGTGAC. The allele gkDf17 was identified by CGH but not confirmed by PCR. Left flanking probe: ATTCCGCGATGTCTCCTTAAATCTTTTGGCAGAGGTTCTCGATTATCCAT. Right flanking probe: ATTGATCGAAAGTTACGAAGACGTGGACTAGTCCCAAAATTCCTAGTGAC. Left deleted probe: GAAAATAGATTTCTACCACTGAACTGTTTTTCTTAACAAACTCATCGAAT. Right deleted probe: CTGTTGAGAACATATCTAGTATTAAGGAAGGAGGGAACTATTCCACAGGC. The allele gkDf18 was identified by CGH but not confirmed by PCR. Left flanking probe: CGAATTTTCGAGGAAGATGAAGTTTATGCGGACGTCCAAAGTGTTGAAAA. Right flanking probe: GATTTCGCTGTGATAAGCGTCGAGGAGGCAATCGAAATGTGGAGCTTCTG. Left deleted probe: CCAAAGTGTTGAAAAACGGAAAATTCAGGATTTCGACGAGCGAATTGAGG. Right deleted probe: CAATTATGCAAATCTCGTCGATATTATACAAAATGATATAGATTTCGCTG. The allele gk3035 was identified by CGH but not confirmed by PCR. Left flanking probe: TGTTTCAGTATTGCCGTCTTATTATGTATAGATTTGCTATTCCATTTCTA. Right flanking probe: CATTTTCGAGTTCAATTTTCTGTGCAAACGCTGGAATGACAATATTCATG. Left deleted probe: TTTATCGTCCCATTAGCATTGTCACTTTTCAATGTTACTACAGTAGGATT. Right deleted probe: TTGTACGAAGGAGAGGAATATGCAAAGTTGAATGCTATTATTCATCTGTC.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037302 Copy
http://www.wormbase.org/db/get?name=WBStrain00037387
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00022632(ZC581.2)
Genomic Alteration: WBGene00022632(ZC581.2)
Availability: available
References:
Synonyms: ZC581.2(gk1146) I.
Alternate IDs: WB-STRAIN:VC2530, CGC_VC2530
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZC581.2. External left primer: TGTCTCCCAACTTCTCGTCC. External right primer: CAATGAAGAATGATCGGGCT. Internal left primer: GGAAACTTTGGAGCGTTCTG. Internal right primer: TTTACGACGTGTTCCATCCA. Internal WT amplicon: 1387 bp. Deletion size: 95 bp. Deletion left flank: TTGATCAATGTGAAATTTTGCGCAGAATCA. Deletion right flank: TTCCCGGAAGATGTTGTCATCAAAATTGAG."
Proper citation: RRID:WB-STRAIN:WBStrain00037387 Copy
http://www.wormbase.org/db/get?name=WBStrain00037388
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018569(F47F2.1)
Genomic Alteration: WBGene00018569(F47F2.1)
Availability: available
References:
Synonyms: F47F2.1(gk1140) X.
Alternate IDs: WB-STRAIN:VC2531, CGC_VC2531
Notes: F47F2.1. Identified by PCR, validated by CGH. External left primer: TGAAAGTGCTCAACATTCGG. External right primer: CAAGGGGGAGCTATACACCA. Internal left primer: AGCATCACGGTGAGTGGTTA. Internal right primer: CATACATCCGATGCGTTGAG. Internal WT amplicon: 1402 bp. Deletion size: 548 bp. Deletion left flank: AGTAATTGAGAAAGATTTTGGTTGCAATTT. Deletion right flank: TGATGGTCGGAAAGCCCCCATTCCGTGGAA. Insertion Sequence: T.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037388 Copy
http://www.wormbase.org/db/get?name=WBStrain00037305
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004170(pqn-90)
Genomic Alteration: WBGene00004170(pqn-90)
Availability: available
References:
Synonyms: pqn-90(gk1086) IV.
Alternate IDs: WB-STRAIN:VC2394, CGC_VC2394
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y73F8A.8. External left primer: ACAACCCGTGCAAGAAAAAC. External right primer: AAGTGGGACGGAACTGTTTG. Internal left primer: ACAATCGCGTCAGTAGGAGC. Internal right primer: CAGGGTTGTAGGACGTTGGT. Internal WT amplicon: 1894 bp. Deletion size: 519 bp. Deletion left flank: AACTGAAGGTTCTAGGATGACAGTCACAATTTGTGGAGCAACAACTGGAGATTGGCATG CTGATTGGCATTGCTGACATTGTGGAGCAGCTGGTTGCTGACATTGTGGAATACATTGT TGTTGGCACTGTTGAGTCTGTTGGCACGAAGTTTGACATTGTTGGCAAGCTGGTGCAGA TGGAAGTGTGCATTGTGGAGCACACTGTTGCTGGCAAACTGGAGCATACTGCTGGCAAG TATTCTGGCACTGCTGGCACTGTGGAGCTGATGGCTGTTGGCA. Deletion right flank: CACTTGGTAAGTTACTTGCTGAACTGGTTGGGCACATGAGCAGGATGTCTGGACTGGAG CAGTGTTCTGACACGAACAACTGTACTGTTGTGGTTGAGTTGCTTGTTGGCATGAACAA CTTGGTTGAACTTGTGAAGCACAACCACATGATTGACGTTTATCACGAATTGAA. Validation: No CGH probes for gk1086."|"Y73F8A.8. External left primer: ACAACCCGTGCAAGAAAAAC. External right primer: AAGTGGGACGGAACTGTTTG. Internal left primer: ACAATCGCGTCAGTAGGAGC. Internal right primer: CAGGGTTGTAGGACGTTGGT. Internal WT amplicon: 1894 bp. Deletion size: 519 bp. Deletion left flank: AACTGAAGGTTCTAGGATGACAGTCACAATTTGTGGAGCAACAACTGGAGATTGGCATGCTGATTGGCATTGCTGACATTGTGGAGCAGCTGGTTGCTGACATTGTGGAATACATTGTTGTTGGCACTGTTGAGTCTGTTGGCACGAAGTTTGACATTGTTGGCAAGCTGGTGCAGATGGAAGTGTGCATTGTGGAGCACACTGTTGCTGGCAAACTGGAGCATACTGCTGGCAAGTATTCTGGCACTGCTGGCACTGTGGAGCTGATGGCTGTTGGCA. Deletion right flank: CACTTGGTAAGTTACTTGCTGAACTGGTTGGGCACATGAGCAGGATGTCTGGACTGGAGCAGTGTTCTGACACGAACAACTGTACTGTTGTGGTTGAGTTGCTTGTTGGCATGAACAACTTGGTTGAACTTGTGAAGCACAACCACATGATTGACGTTTATCACGAATTGAA. Validation: No CGH probes for gk1086."
Proper citation: RRID:WB-STRAIN:WBStrain00037305 Copy
http://www.wormbase.org/db/get?name=WBStrain00037306
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003167(mec-3)
Genomic Alteration: WBGene00003167(mec-3)
Availability: available
References:
Synonyms: mec-3(gk1126) IV.
Alternate IDs: WB-STRAIN:VC2396, CGC_VC2396
Notes: F01D4.6. Identified by PCR, validated by CGH. External left primer: CGCGTTGAAGTCAGTTGTGT. External right primer: GACTCCTGTTGGATTGGCAT. Internal left primer: CTGCCACATCAGTGTTGCTT. Internal right primer: CAAAGCCTCTCAGTGCGATT. Internal WT amplicon: 2450 bp. Deletion size: 774 bp. Deletion left flank: GAGCAAAGCGTAAAAAATGATTACATCTTT. Deletion right flank: TTTTACTTGACTCTCTGAAAGTCGAACAGA. Insertion Sequence: TTACTT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037306 Copy
http://www.wormbase.org/db/get?name=WBStrain00037392
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006814(unc-82)
Genomic Alteration: WBGene00006814(unc-82)
Availability: available
References:
Synonyms: unc-82(gk1124) IV.
Alternate IDs: WB-STRAIN:VC2535, CGC_VC2535
Notes: B0496.3. Identified by PCR, validated by CGH. External left primer: GATGTTGTCGCATTGTGTCC. External right primer: AACTTGATGGATCTGGTGGC. Internal left primer: TGCGCTTCTAATCGTAAGGC. Internal right primer: GGTTCCTCGTCAGGATCAAA. Internal WT amplicon: 2549 bp. Deletion size: 595 bp. Deletion left flank: AGAAACTAGACATAAATCAAGGTATTACTT. Deletion right flank: ACTAAAAGTAAAGGTTACAATTCCAAATTA. Insertion Sequence: AAATAGACATAAATCAAGGTATT.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037392 Copy
http://www.wormbase.org/db/get?name=WBStrain00037394
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001853(hil-2)
Genomic Alteration: WBGene00001853(hil-2)
Availability: available
References:
Synonyms: hil-2(ok2548) IV/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2537, CGC_VC2537
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y73B6BL.9. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2548 homozygotes (early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TACAAAGGTGGGAGGTACGC. External right primer: GAGCATGATGTGACACCCAC. Internal left primer: GGGGCAAAACTATGAGAGCA. Internal right primer: TTTTGCGCTTTTTCAGTGTG. Internal WT amplicon: 2810 bp. Deletion size: approximately 1700 bp."
Proper citation: RRID:WB-STRAIN:WBStrain00037394 Copy
http://www.wormbase.org/db/get?name=WBStrain00037318
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00022851(ZK1127.4)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00022851(ZK1127.4)
Availability: available
References:
Synonyms: ZK1127.4(ok1940)/mIn1 [mIs14 dpy-10(e128)] II.
Alternate IDs: WB-STRAIN:VC2417, CGC_VC2417
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"ZK1127.4. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok1940 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: GAAAAATGGATTGCCGAAGA. External right primer: GGGAAGTTCGTAATCGGTGA. Internal left primer: TTTCAGGCCAAATGTCCTTC. Internal right primer: CTGACAGCTCACACCACGAT. Internal WT amplicon: 2174 bp. Deletion size: 1250 bp. Deletion left flank: TGGAAGCATTCGTGAAGATTTGTCCGGCTT. Deletion right flank: CCTTCACGGTTGGCCCTGCTTTGAAGCTTG. Insertion Sequence: AGA."
Proper citation: RRID:WB-STRAIN:WBStrain00037318 Copy
http://www.wormbase.org/db/get?name=WBStrain00037312
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000254(bli-4)|WBGene00004858(sma-4)
Genomic Alteration: WBGene00000254(bli-4), WBGene00004858(sma-4)
Availability: available
References:
Synonyms: sma-4(ok3140) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2410, CGC_VC2410
Notes: R12B2.1. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3140 homozygotes (late larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GACGGAAAGGTGTTCCACAT. External right primer: GGTCCGTGCAGAAAATCAGT. Internal left primer: CGCAAGAATATGGAGATGGC. Internal right primer: TGCTCGTACTGCTTCATTGC. Internal WT amplicon: 1288 bp. Deletion size: 718 bp. Deletion left flank: AGAGGTGGCTGCTCTCTCTCTCTGACTTTT. Deletion right flank: TTCGTCCGATCCGGGTACCTAGACTACACT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037312 Copy
http://www.wormbase.org/db/get?name=WBStrain00037313
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000254(bli-4)|WBGene00021660(nol-14)
Genomic Alteration: WBGene00000254(bli-4), WBGene00021660(nol-14)
Availability: available
References:
Synonyms: Y48G1A.4(ok3096) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2411, CGC_VC2411
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y48G1A.4. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3096 homozygotes (early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: TAAACTCGCAAAAATTCGCA. External right primer: TCAAATTGCACAAATTCCGA. Internal left primer: TGAAGTGTTTGCGTACAGCG. Internal right primer: TTTTTGGGTTTTAGGTTTTCCA. Internal WT amplicon: 1221 bp. Deletion size: 520 bp. Deletion left flank: TGCGCACGACTTGACGCGCAAACTTCCCAA. Deletion right flank: GGAAAAGCGCTCTCGGACATTGAAAAATAC. Insertion Sequence: CAAA."
Proper citation: RRID:WB-STRAIN:WBStrain00037313 Copy
http://www.wormbase.org/db/get?name=WBStrain00037398
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00044109(K02E11.10)
Genomic Alteration: WBGene00044109(K02E11.10)
Availability: available
References:
Synonyms: K02E11.10(ok3266) V/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2541, CGC_VC2541
Notes: K02E11.10. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3266 homozygotes (probable embryonic arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AGCAACTTGTCCTTGTTGGG. External right primer: GGAGTGTGCAGCAAATTTCA. Internal left primer: CTTCGAAGCCTCCTTGAGTA. Internal right primer: GCGTCTTGAGGCCATAGTTC. Internal WT amplicon: 1208 bp. Deletion size: 582 bp. Deletion left flank: CCTGAGCAGGCCCTTGCTGATATCCGGCTC. Deletion right flank: GGCAGGCTAAGATCACAACGGATTTCATCT. Insertion Sequence: TTCCCTGAACTCCTTGAGCAGATCCCT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037398 Copy
http://www.wormbase.org/db/get?name=WBStrain00037317
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00009559(mtx-1)
Genomic Alteration: WBGene00009559(mtx-1)
Availability: available
References:
Synonyms: mtx-1(ok3155) I.
Alternate IDs: WB-STRAIN:VC2415, CGC_VC2415
Notes: F39B2.11. External left primer: GATTTTGTCGTCTCGTGGGT. External right primer: CAGGATAGCAATTGGGGAGA. Internal left primer: AGTAGGTAGGGGGCAAGCAA. Internal right primer: CTTTGTTCGAAATTTTCCGC. Internal WT amplicon: 1278 bp. Deletion size: 371 bp. Deletion left flank: TCTTACCACTGCTGGATACAAATTGTGATG. Deletion right flank: CTTGAAATTCCAAATTCGGAAAAAAATCAA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037317 Copy
http://www.wormbase.org/db/get?name=WBStrain00037315
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006070(str-2)
Genomic Alteration: WBGene00006070(str-2)
Availability: available
References:
Synonyms: str-2(ok3089) V.
Alternate IDs: WB-STRAIN:VC2413, CGC_VC2413
Notes: C50C10.7. External left primer: TCGACCTGTCAAACATCGAA. External right primer: CGCATTTGTGAACCTGTTTG. Internal left primer: AAATCCTCGTCGATAACTTTTGA. Internal right primer: GCACACATATGGGTCTGCTTT. Internal WT amplicon: 1213 bp. Deletion size: 409 bp. Deletion left flank: TCTATCATCTCAAGCTTTTTGGTCAGCCAA. Deletion right flank: TGAATCGAAGTCCGGAAACAAGTAGTTATT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037315 Copy
http://www.wormbase.org/db/get?name=WBStrain00037320
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020086(npr-24)
Genomic Alteration: WBGene00020086(npr-24)
Availability: available
References:
Synonyms: R106.2(ok3192) X.
Alternate IDs: WB-STRAIN:VC2421, CGC_VC2421
Notes: Made_by: Vancouver KO Group|"R106.2. External left primer: ATTTTACTGGTGTCCTGCGG. External right primer: AAAACGGCAAATTCGAAAAA. Internal left primer: GCATGATCTGCTTATCCGGT. Internal right primer: CCGCAATTCGGTCTAAAACT. Internal WT amplicon: 1241 bp. Deletion size: 738 bp. Deletion left flank: CAACCAACGCTGTGTTGGTTTGTACATATA. Deletion right flank: AAGTTTAACATCTCAAAAGAATTGACTAAG. Insertion Sequence: T."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037320 Copy
http://www.wormbase.org/db/get?name=WBStrain00037401
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000422(ced-8)|WBGene00003056(lon-2)
Genomic Alteration: WBGene00000422(ced-8), WBGene00003056(lon-2)
Availability: available
References:
Synonyms: +/szT1 [lon-2(e678)] I; ced-8(ok3213)/szT1 X.
Alternate IDs: WB-STRAIN:VC2547, CGC_VC2547
Notes: F08F1.5. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3213 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CAAATATCAGCACCAATGCG. External right primer: TGCAATGTGCTCCTATGCTC. Internal left primer: CTTACCTGCAAAACCGCTTC. Internal right primer: CAATCTTTCATTTTTGGGCG. Internal WT amplicon: 1179 bp. Deletion size: 649 bp. Deletion left flank: CTTTCTCAATCTTACCTGCAAAACCGCTTC. Deletion right flank: GTGACCGCAAACTGATTAGTCTCTTGAAAT. Insertion Sequence: ACCGCAAAC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037401 Copy
http://www.wormbase.org/db/get?name=WBStrain00037366
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00008842(chil-28)|WBGene00011802(T16G1.9)|WBGene00013872(ZC374.2)
Genomic Alteration: WBGene00008842(chil-28), WBGene00011802(T16G1.9), WBGene00013872(ZC374.2)
Availability: available
References:
Synonyms: F15A4.8(gk3032) II; T16G1.9(gk3033) V; ZC374.2(gk1152) X.
Alternate IDs: WB-STRAIN:VC2499, CGC_VC2499
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZC374.2, F15A4.8, T16G1.9. The allele gk1152 was identified by PCR, validated by CGH, and can be detected with the following PCR primers. External left primer: TTGGAAGTTTTGGCAGGAAT. External right primer: CTTGCGTTAATCGCATGTGT. Internal left primer: TCCAATTTGAGCGATCAGTG. Internal right primer: AGGACGCGCAGATTGTTAGT. Internal WT amplicon: 2448 bp. Deletion size: 842 bp. Deletion left flank: TCAATGTTCTACTTTTTAACGCATTTACGT. Deletion right flank: GGTTTAGAAGATAACTTTAAATGTTTAAAC. The allele gk3032 was identified by CGH but not confirmed by PCR. Left flanking probe: TCCATAATTCTAGCGACGTTGAAGTTTATCTGTGGTTCATGGCCGGAGTA. Right flanking probe: GTCGTAATTCAGAAAGAAACTCTGAAACCATGTGCTGGTTGGATTCCAGC. Left deleted probe: ATCTGTGGTTCATGGCCGGAGTACAGTGGAAGAGGACCAATTAGTGAACT. Right deleted probe: TTGAGATTAGATACTGGGTTTGCAGAGCCTGTCGTAATTCAGAAAGAAAC. The allele gk3033 was identified by CGH but not confirmed by PCR. Left flanking probe: CGAAGCAGGAGGTCACTTGTTTTGCTTTCCGATAATAATTGAATATCTAG. Right flanking probe: GGATAACCAAACATGTTGAAATTGGCCACGGACGCGTAGCATTCTAAAGA. Left deleted probe: GAAACAAAAGGCCAGGCGATAGAAAATAAGGCAGTAAACGTCAATTAATA. Right deleted probe: AATAATTGTTTACCCATTTCTTGTAAATCATGAGGCAATAGTGCTCTGAA."
Proper citation: RRID:WB-STRAIN:WBStrain00037366 Copy
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