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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00040542
Source Database: WormBase (WB)
Affected Genes: WBGene00002003(hse-5)|WBGene00006853(unc-130)
Genomic Alteration: WBGene00002003(hse-5), WBGene00006853(unc-130)
Availability: available
Source References: EMPTY
Synonyms: unc-130(op459) II; hse-5(tm472) III.
Alternate IDs: WB-STRAIN:WS4591, CGC_WS4591
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040542 Copy
http://www.wormbase.org/db/get?name=WBStrain00040544
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: opIs295 II; unc-119(ed3) III.
Alternate IDs: WB-STRAIN:WS4700, CGC_WS4700
Notes: opIs295 [cdc-42p::GFP::cdc-42(genomic)::cdc-42 3'UTR + unc-119(+)] II.
Proper citation: RRID:WB-STRAIN:WBStrain00040544 Copy
http://www.wormbase.org/db/get?name=WBStrain00040639
Source Database: WormBase (WB)
Availability: available
Source References: EMPTY
Synonyms: qaIs8400.
Alternate IDs: WB-STRAIN:XA8400, CGC_XA8400
Notes: qaIs8400 [let-858p::Ov-GST-3 + rol-6(su1006)]. Called AK1 in the reference article. The Ov-GST-3 gene was amplified from genomic DNA of O. volvulus with 1M of the sequence specific primer 5'Klon and 3'Klon (5'Klon: 5'-GGCGTACGATGTCAAGATTTCCTCAACAAG-3'; 3'Klon: 5'-GGTCTAGATTTATTTAGGAATGATTGAATCGGTCG-3'; representing bases 4 - 25 and the complementary sequence of bases 821 - 841 of the published Ov-GST-3 cDNA (AF203814); bold underlines indicate restriction sites for Pfl23II (SplI) and XbaI, respectively; dotted underline indicates the start codon for translation; italics indicates the conserved sequence for the polyadenylation signal for transgenic transcript processing; the 8 5'-nucleotides of primer 3'Klon and the fourteen 5'-nucleotides of primer 5'Klon do not correspond to the template and introduce the sequences to the amplicon), 200 M of each deoxynucleotide (Gibco BRL) and 2.5 units of Taq polymerase (Gibco BRL). After an initial denaturation of 3 minutes at 93C, 35 cycles of annealing at 55C for 1 minute, synthesis at 72C for 2 minutes and a 1 minute denaturation at 93C were performed, followed by a final extension at 72C for 5 minutes. The genomic Ov-GST-3 fragment obtained by PCR (see above) was ligated into the pGEM-T Easy vector (Promega) by TA-cloning, cleaved with the restriction enzymes Pfl23II (SplI) and XbaI (restriction sites introduced by the primer) and inserted between the unique Pfl23II (SplI) and XbaI sites of the vector pPD103.05 (kindly provided by A. Fire). The sequence of the genomic Ov-GST-3 fragment in the resulting plasmid pAK1 was confirmed by automated dye terminator, dideoxy sequencing (ABI Prism 377TM Sequencer, PE Applied Biosystems) using the PCR primers (see above). The pAK1 DNA was injected in combination with the marker plasmid pRF4 [rol-6(su1006)] into the gonads of N2 C. elegans at a concentration of approximately 100 ng/l for each plasmid. Transgenic worms were identified by the selectable Roller marker phenotype and the stable transmitting line AK1ex (AK1 extrachromosomal) was established. Integration of the extrachromosomal arrays was achieved by irradiation of AK1ex worms with 3600 rad (1 rad = 0.01 Gy) of x-rays (x-ray chamber: RUM 9421-070-77002, Philips, Netherlands; dosimeter: PTW-SN4, PTW, Germany). The progeny of these worms was then screened for 100% transmittance of the Roller phenotype to obtain the C. elegans line AK1int (AK1 integrated) with the chromosomally integrated transgenes.
Proper citation: RRID:WB-STRAIN:WBStrain00040639 Copy
http://www.wormbase.org/db/get?name=WBStrain00040635
Source Database: WormBase (WB)
Affected Genes: WBGene00001593(glc-3)
Genomic Alteration: WBGene00001593(glc-3)
Availability: available
Source References: EMPTY
Synonyms: glc-3(ok321) V.
Alternate IDs: WB-STRAIN:XA7400, CGC_XA7400
Notes: Mutagen:UV/TMP|"ZC317.3 Homozygous. Outer Left Sequence: TCAAAATACAGGGGTAGGCG. Outer Right Sequence: ACAATTCCTGGAACTCACGG. Inner Left Sequence: TGAAGAGGTTTTGAAACGCA. Inner Right Sequence: ACTTTCCGAGAGGAATGGGT. Inner Length: 2746. Estimated Deletion Size: 1200. This strain was provided by the C. elegans Gene Knockout Project at OMRF, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: www.mutantfactory.ouhsc.edu/"
Proper citation: RRID:WB-STRAIN:WBStrain00040635 Copy
http://www.wormbase.org/db/get?name=WBStrain00040645
Source Database: WormBase (WB)
Affected Genes: WBGene00006324(sup-17)
Genomic Alteration: WBGene00006324(sup-17)
Availability: available
Source References: EMPTY
Synonyms: sup-17(n316) I; oxIs12 X; wpEx30.
Alternate IDs: WB-STRAIN:XE1090, CGC_XE1090
Notes: oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. wpEx30 [pdi-2p::sup-17 + myo-2p::GFP]. Pick animals with GFP+ pharynx to maintain array. Hypodermal expression of SUP-17 from wpEx30 rescues sup-17(n316). Reference: El Bejjani R, Hammarlund M. Neuron. 2012; 73(2):268-78.
Proper citation: RRID:WB-STRAIN:WBStrain00040645 Copy
http://www.wormbase.org/db/get?name=WBStrain00040644
Source Database: WormBase (WB)
Affected Genes: WBGene00006324(sup-17)
Genomic Alteration: WBGene00006324(sup-17)
Availability: available
Source References: EMPTY
Synonyms: sup-17(n316) I; oxIs12 X; wpEx22.
Alternate IDs: WB-STRAIN:XE1089, CGC_XE1089
Notes: oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. wpEx22 [myo-3p::sup-17 + myo-2p::GFP]. Pick animals with GFP+ pharynx to maintain array. Muscular expression of SUP-17 from wpEx22 rescues sup-17(n316). Reference: El Bejjani R, Hammarlund M. Neuron. 2012; 73(2):268-78.
Proper citation: RRID:WB-STRAIN:WBStrain00040644 Copy
http://www.wormbase.org/db/get?name=WBStrain00040647
Source Database: WormBase (WB)
Availability: available
Source References: EMPTY
Synonyms: wpEx76.
Alternate IDs: WB-STRAIN:XE1220, CGC_XE1220
Notes: wpEx76 [myo-3p::tom20::tdKillerRed + unc-122p::GFP]. Pick GFP+ to maintain. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpEx76 carries a tandem dimer version of KillerRed targeted to the mitochondria by the addition of a tom-20 targeting sequence (mito-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
Proper citation: RRID:WB-STRAIN:WBStrain00040647 Copy
http://www.wormbase.org/db/get?name=WBStrain00040641
Source Database: WormBase (WB)
Affected Genes: WBGene00006324(sup-17)
Genomic Alteration: WBGene00006324(sup-17)
Availability: available
Source References: EMPTY
Synonyms: sup-17(n316) I; oxIs12.
Alternate IDs: WB-STRAIN:XE1026, CGC_XE1026
Notes: oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. Neuron. 2012 Jan 26;73(2):268-78.
Proper citation: RRID:WB-STRAIN:WBStrain00040641 Copy
http://www.wormbase.org/db/get?name=WBStrain00040642
Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)
Genomic Alteration: WBGene00001073(dpy-11)
Availability: available
Source References: EMPTY
Synonyms: geIs3 I; dpy-11(e224) V; oxIs12 X.
Alternate IDs: WB-STRAIN:XE1037, CGC_XE1037
Notes: geIs3 [sir-2.1(+) + rol-6(su1006)] I. oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. Rollers. Dpy. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
Proper citation: RRID:WB-STRAIN:WBStrain00040642 Copy
http://www.wormbase.org/db/get?name=WBStrain00040619
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: unc-119(ed3) qaIs3507 III.
Alternate IDs: WB-STRAIN:XA3507, CGC_XA3507
Notes: Made_by: Askjaer/Mattaj/Galy|"qaIs3507 [unc-119(+) + pie-1p::GFP::lem-2] WT. Stable expression of GFP::LEM-2 when grown at 20C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Commercial requests should be addressed to [email protected]"
Proper citation: RRID:WB-STRAIN:WBStrain00040619 Copy
http://www.wormbase.org/db/get?name=WBStrain00040616
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: unc-119(ed3) III; qaIs3502.
Alternate IDs: WB-STRAIN:XA3502, CGC_XA3502
Notes: Made_by: Askjaer/Mattaj/Galy|"qaIs3502[unc-119(+) + pie-1::YFP::lmn-1 + pie-1::CFP::H2B] Relative stable expression of YFP::LMN-1 when grown at 24C. Expression of CFP::H2B is silenced. qaIs3502 is presumably not on LG III. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Commercial requests should be addressed to [email protected]"
Proper citation: RRID:WB-STRAIN:WBStrain00040616 Copy
http://www.wormbase.org/db/get?name=WBStrain00040615
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: PMID:39652540
Synonyms: unc-119(ed3) ruIs32 III; ojIs1.
Alternate IDs: WB-STRAIN:XA3501, CGC_XA3501
Notes: Made_by: Askjaer/Mattaj|"ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Stable expression of GFP::histoneH2B and GFP::beta-tubulin when grown at 16-25C. ruIs32 contains plasmid pAZ132. ojIs1 is not on LG I, II, or IV."
Proper citation: RRID:WB-STRAIN:WBStrain00040615 Copy
http://www.wormbase.org/db/get?name=WBStrain00040618
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: EMPTY
Synonyms: unc-119(ed3) III; qaEx3504.
Alternate IDs: WB-STRAIN:XA3504, CGC_XA3504
Notes: Made_by: Askjaer/Mattaj/Galy|"qaEx3504 [pie-1::GFP::emr-1 + unc-119(+)]. Maintain by picking WT. Stable expression of GFP::EMR-1 when grown at 20C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Commercial requests should be addressed to [email protected]"
Proper citation: RRID:WB-STRAIN:WBStrain00040618 Copy
http://www.wormbase.org/db/get?name=WBStrain00040617
Source Database: WormBase (WB)
Affected Genes: WBGene00002073(ima-2)
Genomic Alteration: WBGene00002073(ima-2)
Availability: unknown
Source References: EMPTY
Synonyms: ima-2(ok256)/+ I; ojIs1.
Alternate IDs: WB-STRAIN:XA3503
Notes: Made_by: Askjaer/Mattaj|"ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Homozygous ima-2(ok256) worms produce only dead embryos. Maintain heterozygous population by propagating worms that give 25% sterile progeny or by single worm PCR, e.g. with primers 5'-GAAGAGGGAAAGGATGAGGG and 5'-GTTTGATGTTTTCACCGCCT which will give approx. 1.2 kb PCR product from the ok256 allele. Homozygous for pie-1::GFP::beta-tubulin. ojIs1 is not on LG I, II, or IV."
Proper citation: RRID:WB-STRAIN:WBStrain00040617 Copy
http://www.wormbase.org/db/get?name=WBStrain00040692
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:33820969
Synonyms: Caenorhabditis elegans wild isolate.
Alternate IDs: WB-STRAIN:XZ2018
Notes: Added Wild_Isolate tag as Isolation information is available suggesting that they were sampled from the wild.
Proper citation: RRID:WB-STRAIN:WBStrain00040692 Copy
http://www.wormbase.org/db/get?name=WBStrain00040691
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:33820969, PMID:38564369
Synonyms: Caenorhabditis elegans wild isolate.
Alternate IDs: WB-STRAIN:XZ1756
Notes: Added Wild_Isolate tag as Isolation information is available suggesting that they were sampled from the wild.
Proper citation: RRID:WB-STRAIN:WBStrain00040691 Copy
http://www.wormbase.org/db/get?name=WBStrain00040699
Source Database: WormBase (WB)
Availability: available
Source References: EMPTY
Synonyms: yakEx142.
Alternate IDs: WB-STRAIN:XZ2080, CGC_XZ2080
Notes: yakEx142 [unc-14p::hif-1(cDNA) + myo-2p::mCherry]. Pick animals with red pharynx to maintain. unc-14 promoter drives GFP expression in several tissues including neurons, intestine, muscle, hypodermis. Reference: Topalidou I & and Miller DL. bioRxiv 174581; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00040699 Copy
http://www.wormbase.org/db/get?name=WBStrain00040614
Source Database: WormBase (WB)
Affected Genes: WBGene00003056(lon-2)|WBGene00003907(paf-2)
Genomic Alteration: WBGene00003056(lon-2), WBGene00003907(paf-2)
Availability: available
Source References: EMPTY
Synonyms: paf-2(tj12)/lon-2(e678) X.
Alternate IDs: WB-STRAIN:XA3102, CGC_XA3102
Notes: Heterozygotes are WT and segregate WT, Lon and dead eggs. Maintain by picking WT.|"Mutagen:UV/TMP"
Proper citation: RRID:WB-STRAIN:WBStrain00040614 Copy
http://www.wormbase.org/db/get?name=WBStrain00040613
Source Database: WormBase (WB)
Affected Genes: WBGene00003906(paf-1)
Genomic Alteration: WBGene00003906(paf-1)
Availability: available
Source References: EMPTY
Synonyms: paf-1(tj11) I.
Alternate IDs: WB-STRAIN:XA3101, CGC_XA3101
Notes: Mutagen:UV/TMP|"Superficially WT."
Proper citation: RRID:WB-STRAIN:WBStrain00040613 Copy
http://www.wormbase.org/db/get?name=WBStrain00040695
Source Database: WormBase (WB)
Affected Genes: WBGene00001851(hif-1)
Genomic Alteration: WBGene00001851(hif-1)
Availability: available
Source References: EMPTY
Synonyms: hif-1(ia4) V; yakEx126.
Alternate IDs: WB-STRAIN:XZ2056, CGC_XZ2056
Notes: yakEx126 [unc-17p::hif-1(cDNA) + myo-2p::mCherry]. Pick animals with red pharynx to maintain. HIF-1 expressed from cholinergic neuron-specific promoter in hif-1 mutant background for tissue-specific rescuing experiments. yakEx126 does not rescue lethality in hif-1 mutant animals exposed to 50ppm hydrogen sulfide. Reference: Topalidou I & and Miller DL. bioRxiv 174581; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00040695 Copy
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