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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00051456
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00017553(nep-8)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00017553(nep-8)
Availability: unknown
Source References: EMPTY
Synonyms: nep-8(gk5859[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) II.
Notes: Homozygous viable. Deletion of 2935 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking sequence: AAGAAAGTGAAAGAGAAGCTCTCTAGGCCT. Right flanking sequence: aggttcatccggcgacgacaaaaaatctaa. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051456 Copy
http://www.wormbase.org/db/get?name=WBStrain00051455
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00012187(rpb-11)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00012187(rpb-11)
Availability: unknown
Source References: EMPTY
Synonyms: rpb-11(gk5858[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ II.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 413 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: ATCATTTTTGCTTCTCGACGACAAGAAATT. Right flanking sequence: AACTGATTTTTCACTTTTTCCTTACTGATC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051455 Copy
http://www.wormbase.org/db/get?name=WBStrain00051454
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00022797(best-24)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00022797(best-24)
Availability: unknown
Source References: EMPTY
Synonyms: best-24(gk5857[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) III.
Notes: Homozygous viable. Deletion of 3283 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking sequence: TACAACTTAGCAGTATCCACTTCAAAACCG. Right flanking sequence: GAGAAACTACCCGATCCACCCGACCACAAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051454 Copy
http://www.wormbase.org/db/get?name=WBStrain00051453
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: Y71G12B.13(gk5855[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ I.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 5445 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: CACTGCAACAATCGCAATTTTCAGACAATT. Right flanking sequence: CGGAGCCTGTGCAATTCCATGTGGGAAATT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051453 Copy
http://www.wormbase.org/db/get?name=WBStrain00051458
Source Database: WormBase (WB)
Affected Genes: WBGene00001693(grd-4)|WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00001693(grd-4), WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: grd-4(gk5861[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP]) X.
Notes: Homozygous viable. Deletion of 890 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking sequence: gcaatacaacgaagtcatctaaagtgttca. Right flanking sequence: tgtgtgtttttgagaggataaaaagaaaca. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051458 Copy
http://www.wormbase.org/db/get?name=WBStrain00051444
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: C50F2.2(gk5846[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ I.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 4481 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: TACCATCAGTTCATGAGAATGCCTACCAAG. Right flanking sequence: ACTGGAATTATGGCACAGATTATGAGGAAG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051444 Copy
http://www.wormbase.org/db/get?name=WBStrain00051442
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006383(taf-2)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006383(taf-2), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: taf-2(gk5844[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ IV.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 8143 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: CGCCTCGTATCCACTATTATGCCACTGTGA. Right flanking sequence: CGTTCAGCGTCACTTTGCTCGCCGAGCCCG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051442 Copy
http://www.wormbase.org/db/get?name=WBStrain00051447
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: Y65B4A.8(gk5849[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ I.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 3386 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: GACGGTCCACGTTTCGGCGAGCGTTTTGTG. Right flanking sequence: CGGCTGCGCGTCTCTATTTCACACACTGTC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051447 Copy
http://www.wormbase.org/db/get?name=WBStrain00051446
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00013877(ints-2)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00013877(ints-2)
Availability: unknown
Source References: EMPTY
Synonyms: ints-2(gk5848[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ V.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 5928 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: GGGAAAGAAAGAAAAATTACTTCGAGATTG. Right flanking sequence: TGGAATGTTGGCTGGAGATCCACGACATGA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051446 Copy
http://www.wormbase.org/db/get?name=WBStrain00051438
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00010993(vha-20)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00010993(vha-20)
Availability: unknown
Source References: EMPTY
Synonyms: vha-20(gk5840[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ X.
Notes: Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 1452 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: TTAGACGGTTATCAAGTTTTTATGAAACCA. Right flanking sequence: AACTTCATCCTGAAATTGATTGATTGAGAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: Vancouver KO Group"
Proper citation: RRID:WB-STRAIN:WBStrain00051438 Copy
http://www.wormbase.org/db/get?name=WBStrain00051539
Source Database: WormBase (WB)
Affected Genes: WBGene00011765(frpr-17)
Genomic Alteration: WBGene00011765(frpr-17)
Availability: unknown
Source References: PMID:34741504
Synonyms: frpr-17(sy1302) X.
Notes: Batch Strain object requested via get_NS_ids.pl|"Made_by: Heenam Park & Mandy Tan"|"Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of frpr-17. Universal 43 bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: GCAGTTAATCAGTCATGATATTATCGATCCAAGCT right flanking sequence: CAGTGGGATTTCAAAACTGTGAACCTTTGgtgag inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TATTATCGATCCAAGCTCAG Method Reference: G3 (Bethesda).2018 Nov 6;8(11):3607-3616"
Proper citation: RRID:WB-STRAIN:WBStrain00051539 Copy
http://www.wormbase.org/db/get?name=WBStrain00051538
Source Database: WormBase (WB)
Affected Genes: WBGene00019496(frpr-14)
Genomic Alteration: WBGene00019496(frpr-14)
Availability: unknown
Source References: PMID:34741504
Synonyms: frpr-14(sy1301)
Notes: Batch Strain object requested via get_NS_ids.pl
Proper citation: RRID:WB-STRAIN:WBStrain00051538 Copy
http://www.wormbase.org/db/get?name=WBStrain00051593
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: bqSi1030 II; bqSi508 IV.
Notes: bqSi1030 [hsp16.41p::FRT::mCherry::his-58::FRT::vhhGFP4::zif-1 + unc-119(+)] II. bqSi508 [elt-2p::FLP + unc-119(+)] IV. Can be used for inducible degradation of GFP fusion proteins in intestinal cells. May carry unc-119(ed3) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00051593 Copy
http://www.wormbase.org/db/get?name=WBStrain00051592
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: bqSi1030 II; bqSi548 IV.
Notes: bqSi1030 [hsp16.41p::FRT::mCherry::his-58::FRT::vhhGFP4::zif-1 + unc-119(+)] II. bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Can be used for inducible degradation of GFP fusion proteins in hypodermal cell. May carry unc-119(ed3) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00051592 Copy
http://www.wormbase.org/db/get?name=WBStrain00051599
Source Database: WormBase (WB)
Affected Genes: WBGene00002245(lag-1)
Genomic Alteration: WBGene00002245(lag-1)
Availability: unknown
Source References: EMPTY
Synonyms: ieSi64 II; lag-1(oz536oz537[lag-1::degron::3xHA]) IV.
Notes: ieSi64 [gld-1p::TIR1::mRuby::gld-1 3'UTR + Cbr-unc-119(+)] II. Essentially wild-type when maintained on NGM plates. All germline stem cells enter into meiosis when treated with Auxin (1mM). References: Chen J, et al. PLoS Genet. 2020 Mar 20;16(3):e1008650. PMID: 32196486. Chen J, et al. 2020 MicroPublication (https:|"Made_by: Jian Chen"
Proper citation: RRID:WB-STRAIN:WBStrain00051599 Copy
http://www.wormbase.org/db/get?name=WBStrain00051596
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: bqSi294 II; bqSi1308 IV.
Notes: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi1308 [hlh-12p::FLP::SL2::mTagBFP2 + unc-119(+)] IV. Heat shock induces nuclear GFP expression in the distal tip cells and nuclear mCherry expression elsewhere. Constitutive expression of diffusible mTagBFP2 in the distal tip cells. Might carry unc-119(ed3) or unc-119(ed9) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00051596 Copy
http://www.wormbase.org/db/get?name=WBStrain00051636
Source Database: WormBase (WB)
Affected Genes: WBGene00006654(ttx-3)|WBGene00009100(rgef-1)
Genomic Alteration: WBGene00006654(ttx-3), WBGene00009100(rgef-1)
Availability: unknown
Source References: EMPTY
Synonyms: juEx4586 [rgef-1p::GFP1-10 + ttx-3p::RFP].
Notes: juEx4586 [rgef-1p::GFP1-10 + ttx-3p::RFP]. Pick RFP+ to maintain. RFP expression in AIY neuron. Weak light green signals were observed in the neurons using compound scope although this is only the former half of the split GFP. Reference: Noma K, et al. Elife. 2017 Aug 2;6:e26376. doi: 10.7554/eLife.26376.|"Made_by: Ken Noma"
Proper citation: RRID:WB-STRAIN:WBStrain00051636 Copy
http://www.wormbase.org/db/get?name=WBStrain00051635
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: rjSi1 II.
Notes: rjSi1 [cra-1p::cra-1::GFP::cra-1 3'UTR + Cbr-unc-119(+)] II. Single copy insertion. cra-1 promoter, cra-1::GFP and 3'UTR was cloned into pCFJ150 (ttTi5605) vector and inserted into ttTi5605 of EG4322 strain. Outcrossed three times to N2 Bristol; could still carry unc-119(ed9) in the background. Superficially wild-type. This CRA-1::GFP fusion construct has been shown to be functional and its localization reflects endogenous CRA-1 localization. rjSi1 transgene can rescue synapsis defects of cra-1 mutants and restore cross-over events (six bivalents instead of the 11 to 12 univalents characteristic of cra-1 mutants). Brood size and embryonic lethality were significantly, albeit not completely, restored in the rescued line suggesting that the GFP tag might affect other CRA-1 functions. Reference: Gao J, et al. PLOS Genetics 11(3): e1005029. https:
Proper citation: RRID:WB-STRAIN:WBStrain00051635 Copy
http://www.wormbase.org/db/get?name=WBStrain00051634
Source Database: WormBase (WB)
Affected Genes: WBGene00002957(let-858)|WBGene00003310(mir-82)
Genomic Alteration: WBGene00002957(let-858), WBGene00003310(mir-82)
Availability: unknown
Source References: EMPTY
Synonyms: mir-82(umn87[mir-82p+SL1::EGL13NLS::lox2272mScarlet-I::cMycNLS::let-858 3' UTR::lox2272]) X.
Notes: Made_by: Julie Knott and Marcus L. Vargas|"Nuclear mScarlet-I was inserted in place of the endogenous mir-82 pre-miRNA via CRISPR/CAS9. Left Flanking: TATCATTCTCTCTACTACTAGTGAACTCAT, Right Flanking: TTATCAAGAAAATTCAAGAAAATTCAAAAG. sgRNA: CTGTAGATCACAGAGAAAAC."
Proper citation: RRID:WB-STRAIN:WBStrain00051634 Copy
http://www.wormbase.org/db/get?name=WBStrain00051584
Source Database: WormBase (WB)
Affected Genes: WBGene00001569(meg-3)|WBGene00016485(meg-4)
Genomic Alteration: WBGene00001569(meg-3), WBGene00016485(meg-4)
Availability: unknown
Source References: EMPTY
Synonyms: bqSi577 IV; meg-3(tm4259) meg-4(ax2026) X.
Notes: bqSi577 [myo-2p::GFP + unc-119(+)] IV. Germ granule defective, ~30 sterility. Express GFP in pharyngeal muscles. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343|"Made_by: Itai Toker"
Proper citation: RRID:WB-STRAIN:WBStrain00051584 Copy
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