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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037443
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00018838(F54G2.2)
Genomic Alteration: WBGene00018838(F54G2.2)
Availability: available
References:
Synonyms: F54G2.2(ok3352) X.
Alternate IDs: WB-STRAIN:VC2607, CGC_VC2607
Notes: F54G2.2. External left primer: AATCAGTTAAAGGGGTGGGG. External right primer: CAGCAACAAACTCAGCCAAA. Internal left primer: AATGGTGGGTGGTTGTCTGT. Internal right primer: TGTTCACACTTTAGATGTATTTCCG. Internal WT amplicon: 1153 bp. Deletion size: 625 bp. Deletion left flank: ACGAGATGGAAAGACCATCATCCTGCTTCT. Deletion right flank: CAGAGCTGAATTCCCATTTTTCCATATTCA. Insertion Sequence: T.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037443 Copy
http://www.wormbase.org/db/get?name=WBStrain00037449
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00011735(hip-1)
Genomic Alteration: WBGene00011735(hip-1)
Availability: available
References:
Synonyms: T12D8.8(gk1134) III.
Alternate IDs: WB-STRAIN:VC2613, CGC_VC2613
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"T12D8.8. Identified by PCR, validated by CGH. External left primer: CGAATCGATCCGATCTTCAT. External right primer: GGGATCGATAATGGCTCAGA. Internal left primer: GTTTCCGGAGTTGGAACTGA. Internal right primer: TTGCGAACAACAAATCCTCA. Internal WT amplicon: 1279 bp. Deletion size: 764 bp. Deletion left flank: AAGAAATCAACACAATTTAATGTTAAAGAT. Deletion right flank: GAACCCGCTCTCTACGCTCCGCGAGCTCGA."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037449 Copy
http://www.wormbase.org/db/get?name=WBStrain00037446
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00002253(lbp-1)
Genomic Alteration: WBGene00002253(lbp-1)
Availability: available
References:
Synonyms: lbp-1(ok3426) X.
Alternate IDs: WB-STRAIN:VC2610, CGC_VC2610
Notes: F40F4.3. External left primer: TTGGTTTTCCAAAGTCCCAG. External right primer: GAATCACAAAAGAACCGCGT. Internal left primer: CTGCATGGATTGTGTTTTGAA. Internal right primer: TGCCCCATATCACATTACAGA. Internal WT amplicon: 1207 bp. Deletion size: 398 bp. Deletion left flank: CGGGGTCCACGCAGACGACGTCGACACACA. Deletion right flank: TTGATTAATTAATTAATTTTCAGATCACTT. Insertion Sequence: T.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037446 Copy
http://www.wormbase.org/db/get?name=WBStrain00037447
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00007810(C29F7.1)
Genomic Alteration: WBGene00007810(C29F7.1)
Availability: available
References:
Synonyms: C29F7.1(ok3434) X.
Alternate IDs: WB-STRAIN:VC2611, CGC_VC2611
Notes: C29F7.1. External left primer: CAAAGCTGGGTGAAGGTGTT. External right primer: CATAAGATTGGCATCTCGCA. Internal left primer: GATGTTAACAAAGGCAACGC. Internal right primer: AGGTTTTCCATCGGTCTGAA. Internal WT amplicon: 1264 bp. Deletion size: 309 bp. Deletion left flank: AAAAAGTTTTTTTAGAACTTTTTTATTTAG. Deletion right flank: AAGTCCCATGGAAGATCTCCATAGAATTTT. Insertion Sequence: TTGGTCATCAGGA.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037447 Copy
http://www.wormbase.org/db/get?name=WBStrain00037452
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001333(erm-1)|WBGene00003056(lon-2)
Genomic Alteration: WBGene00001333(erm-1), WBGene00003056(lon-2)
Availability: available
References:
Synonyms: erm-1(ok3269)/szT1 [lon-2(e678)] I; +/szT1 X.
Alternate IDs: WB-STRAIN:VC2617, CGC_VC2617
Notes: C01G8.5. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3269 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTTGAGTGTGTTGTTGCGA. External right primer: GCGCACATCCTTTTTCATTT. Internal left primer: ACAATCAGGGATTCCGTTTT. Internal right primer: TGGATGGAACATTTTGTGGA. Internal WT amplicon: 1263 bp. Deletion size: 1068 bp. Deletion left flank: GAAAACATTTAAAAAAATGTTTATCAAAAA. Deletion right flank: CATTTTTTCGATTTTTTTTTCAGCGAAAAT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037452 Copy
http://www.wormbase.org/db/get?name=WBStrain00037456
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00003775(nmr-2)
Genomic Alteration: WBGene00003775(nmr-2)
Availability: available
References:
Synonyms: nmr-2(ok3324) V.
Alternate IDs: WB-STRAIN:VC2623, CGC_VC2623
Notes: T01C3.10. External left primer: CGTTACTTTTCTCGCCAAGG. External right primer: TCGTGCAAAAGTGAAGATGG. Internal left primer: CTTGCACTGAACAATGTCGC. Internal right primer: CCATATTGGGACAATGGGAA. Internal WT amplicon: 1286 bp. Deletion size: 597 bp. Deletion left flank: TACAATTATTGAAATTCCAGTATCCAAAAA. Deletion right flank: GCTCCCTGAGGTGGATTGGCCTTTTCGCCC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"WBStrain provided so WBPaper00061672 paper added based on AFP_Strain data."
Proper citation: RRID:WB-STRAIN:WBStrain00037456 Copy
http://www.wormbase.org/db/get?name=WBStrain00037453
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00013724(Y106G6H.14)
Genomic Alteration: WBGene00013724(Y106G6H.14)
Availability: available
References:
Synonyms: Y106G6H.14(gk1137) I.
Alternate IDs: WB-STRAIN:VC2619, CGC_VC2619
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y106G6H.14. Identified by PCR, validated by CGH. External left primer: GGATGCTAGTTTGGAGAGCG. External right primer: AACAGCTGACAAGGAGCGAT. Internal left primer: GTGAACCATCCGATTATGCC. Internal right primer: AATTCGAGAAGAACGATGCG. Internal WT amplicon: 1747 bp. Deletion size: 767 bp. Deletion left flank: CGTTATTCAGCCGCAAAATTAGAGAAATCT. Deletion right flank: AGACATTCAGCCAGCATATCCATATTTCCA. Insertion Sequence: CGACTTTCGCGACG."
Proper citation: RRID:WB-STRAIN:WBStrain00037453 Copy
http://www.wormbase.org/db/get?name=WBStrain00037459
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00010070(nep-17)
Genomic Alteration: WBGene00010070(nep-17)
Availability: available
References:
Synonyms: F54F11.2(ok3251) III.
Alternate IDs: WB-STRAIN:VC2627, CGC_VC2627
Notes: F54F11.2. External left primer: AGTGGTACTGTAGGCCGGTG. External right primer: TCGGAGATCATAGGGCATTC. Internal left primer: TCCTACGCCTGTGGAAACTT. Internal right primer: TTGCATAGGCCTTCTGCTTT. Internal WT amplicon: 1216 bp. Deletion size: 472 bp. Deletion left flank: AGGATCCAACTTACCAGACCACTATCAATA. Deletion right flank: CTATGAGCAGAACATTGCAGTCAAGTACAG.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037459 Copy
http://www.wormbase.org/db/get?name=WBStrain00037463
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00004457(rpm-1)|WBGene00009109(degt-1)
Genomic Alteration: WBGene00004457(rpm-1), WBGene00009109(degt-1)
Availability: available
References:
Synonyms: rpm-1(ju1928) degt-1(ok3307) V.
Alternate IDs: WB-STRAIN:VC2633, CGC_VC2633
Notes: F25D1.4. External left primer: TCAAGGAAGCATCCGAAGTT. External right primer: CCACGGATGAATCGAGTTTT. Internal left primer: TCAGATTTTTGGAGTTTCCGA. Internal right primer: TTATTCGATTTTCCCCGTTG. Internal WT amplicon: 1152 bp. Deletion size: 915 bp. Deletion left flank: TTTTGGAGTTTCCGATAATTTCCATGATGT. Deletion right flank: TTCAGTGATAAATTTTCAAATTTCTCGAAA. [NOTE: (05/10/2022) This strain also carries an (A to T) missense mutation in rpm-1 which results in a Q3089H amino acid substitution in RPM-1. See Jin EJ & Jin Y. (2022). A mutation linked to degt-1(ok3307) in C. elegans strain VC2633 affects rpm-1. microPublication Biology. 10.17912/micropub.biology.000565. PMC ID: PMC9073554.]|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037463 Copy
http://www.wormbase.org/db/get?name=WBStrain00037428
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017921(F29B9.5)
Genomic Alteration: WBGene00017921(F29B9.5)
Availability: available
References:
Synonyms: F29B9.5(ok3387) IV.
Alternate IDs: WB-STRAIN:VC2586, CGC_VC2586
Notes: F29B9.5. External left primer: CTGTGAAGTATGCTGCCGAA. External right primer: TGGCAGGTACAATCTAGGGC. Internal left primer: TATTTCTGTATGCGGCAACG. Internal right primer: GGGCAAACCTAGAGAAAAACTATT. Internal WT amplicon: 1213 bp. Deletion size: 670 bp. Deletion left flank: CAACAATGTCCGAATTCATGAAAGGTGTGA. Deletion right flank: ATTATCACGTAAATATTTATATTTTAATAG.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037428 Copy
http://www.wormbase.org/db/get?name=WBStrain00037429
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00008006(tag-325)
Genomic Alteration: WBGene00008006(tag-325)
Availability: available
References:
Synonyms: tag-325(ok1330) III.
Alternate IDs: WB-STRAIN:VC2587, CGC_VC2587
Notes: C38D4.5. External left primer: TTTAAAAGCTTCAGCCGACC. External right primer:GCTGTCGTTCCGTCACTATG. Internal left primer: TCGGACGGACATTTTTCTTC. Internal right primer: ACAGAGCAACGGAAATTTGG. Internal WT amplicon: 3386 bp. Deletion size: 2724 bp. Deletion left flank: GAACAAAATGCGTGAATCTTTAGCTGATGA. Deletion right flank: AATTCCAATGTGAGTTTTTTTTTCAAAAAA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037429 Copy
http://www.wormbase.org/db/get?name=WBStrain00037423
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00015670(C10B5.1)
Genomic Alteration: WBGene00015670(C10B5.1)
Availability: available
References:
Synonyms: C10B5.1(ok3270) V/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2579, CGC_VC2579
Notes: C10B5.1. Homozygous sterile deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok3270 homozygotes (sterile adult). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GCATCCCGATGATCTCATTT. External right primer: GCGTCAAATATGGTGAGCAA. Internal left primer: CATTTCATGGTTTTGCTCCA. Internal right primer: TTCTCAGAATTTAGTGTTTCCGT. Internal WT amplicon: 1224 bp. Deletion size: 573 bp. Deletion left flank: TCGTAGTGTGACGTCATTCTACAGTTTAGA. Deletion right flank: CAACAAAATCGAATCGAATTCTGGATGAAG.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037423 Copy
http://www.wormbase.org/db/get?name=WBStrain00037426
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00006953(wrt-7)
Genomic Alteration: WBGene00006953(wrt-7)
Availability: available
References:
Synonyms: wrt-7(ok3271) V.
Alternate IDs: WB-STRAIN:VC2584, CGC_VC2584
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"ZK1037.10. External left primer: CTTTGTCAAGGCCTGAAAGC. External right primer: GTTTTCGACGACATCCCTGT. Internal left primer: TCACTTCTGACTGTGCCAGG. Internal right primer: CATGTGAGCCAGCTTTTCAA. Internal WT amplicon: 1238 bp. Deletion size: 701 bp. Deletion left flank: TCACACTGATATGTATTAGAACCCGCGGGG. Deletion right flank: AATGTTCTTCGCAATGTTACTAATGTAATC. Insertion Sequence: TTCTAATACATATCAG."
Proper citation: RRID:WB-STRAIN:WBStrain00037426 Copy
http://www.wormbase.org/db/get?name=WBStrain00037424
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001072(dpy-10)|WBGene00006381(tac-1)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00006381(tac-1)
Availability: available
References:
Synonyms: tac-1(ok3305)/mT1 II; +/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC2580, CGC_VC2580
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y54E2A.3. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3305 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AATTCGCTCAAAATCCATGC. External right primer: AAAATAAATGATGACGCGGG. Internal left primer: ATCAAAACAAATTCGGCCTG. Internal right primer: TTTTCACGAAAAATGTCGGTT. Internal WT amplicon: 1236 bp. Deletion size: 812 bp. Deletion left flank: CGCTGTATCTTTGGCGCGAAAATTTAGAAG. Deletion right flank: TTTAGCAATTTTTCAAAGCTTCTCACCATC. Insertion Sequence: CAATTTTTCAGCAATTTTAGCAA."
Proper citation: RRID:WB-STRAIN:WBStrain00037424 Copy
http://www.wormbase.org/db/get?name=WBStrain00037430
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00020293(nep-20)
Genomic Alteration: WBGene00020293(nep-20)
Availability: available
References:
Synonyms: nep-20(ok3259) II.
Alternate IDs: WB-STRAIN:VC2588, CGC_VC2588
Notes: Made_by: Vancouver KO Group|"T06D4.4. External left primer: CGAATTGTGGCATGTTCTTG. External right primer: GGTAAATAGGCAGGCGTGAA. Internal left primer: GTCGAGTAATGTTGGCGAGC. Internal right primer: CATGAAGTAGGCACGCATGT. Internal WT amplicon: 1135 bp. Deletion size: 381 bp. Deletion left flank: AAGTAAGGGCCATCAATGATCGGAGCAATG. Deletion right flank: CGGAGAAAAAGCATTAAAACAACCTTTATC."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037430 Copy
http://www.wormbase.org/db/get?name=WBStrain00037433
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00001445(flp-2)
Genomic Alteration: WBGene00001445(flp-2)
Availability: available
References:
Synonyms: flp-2(ok3351) X.
Alternate IDs: WB-STRAIN:VC2591, CGC_VC2591
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"W07E11.3. External left primer: TCAACTCTCACACAGCCCAC. External right primer: ATTTTCAGGTACACACCCGC. Internal left primer: GTTGGTTGAGATGCCACCTT. Internal right primer: CACAGAGCTTTCGTCTGACTC. Internal WT amplicon: 1219 bp. Deletion size: 372 bp. Deletion left flank: TTCCAAATATGTGTTTGGGTTTTAAGCTTG. Deletion right flank: CGACAATTGGTTTGGCAACGACTGACAATG."
Proper citation: RRID:WB-STRAIN:WBStrain00037433 Copy
http://www.wormbase.org/db/get?name=WBStrain00037431
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00022189(Y71H2AR.2)
Genomic Alteration: WBGene00022189(Y71H2AR.2)
Availability: available
References:
Synonyms: Y71H2AR.2(ok3282) III.
Alternate IDs: WB-STRAIN:VC2589, CGC_VC2589
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y71H2AR.2. External left primer: AAAACCACTCCTTTGGTCCC. External right primer: CGTTGCAATGGGGTAAGTCT. Internal left primer: CCCAATTGGCGCTACTCTAA. Internal right primer: TTTTATACAAACGAAGAAGGCCTAA. Internal WT amplicon: 1156 bp. Deletion size: 794 bp. Deletion left flank: TATGGATAGTCTGCTTCTGTTTCTATGCCA. Deletion right flank: GAATTGAAGGTGCTTCTCGAGTTTGCTGGC."
Proper citation: RRID:WB-STRAIN:WBStrain00037431 Copy
http://www.wormbase.org/db/get?name=WBStrain00037435
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00017646(F20H11.1)
Genomic Alteration: WBGene00017646(F20H11.1)
Availability: available
References:
Synonyms: F20H11.1(ok3386) III.
Alternate IDs: WB-STRAIN:VC2594, CGC_VC2594
Notes: F20H11.1. External left primer: CAGCAACTCCATCAAAGCAA. External right primer: CGTTTCTGCCGATTTTTCAT. Internal left primer: AGTTGACAGAACTCCGGCAC. Internal right primer: TTTTGGCTAGAGAATCACAAAAA. Internal WT amplicon: 1279 bp. Deletion size: 472 bp. Deletion left flank: GTGCAAAAAAAACAATTTCTCCAGACCGGG. Deletion right flank: AAGGAGTTGAAGATTGATTATGAGCATCTT.|"Made_by: Vancouver KO Group"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037435 Copy
http://www.wormbase.org/db/get?name=WBStrain00037436
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00022663(glrx-21)
Genomic Alteration: WBGene00022663(glrx-21)
Availability: available
References:
Synonyms: glrx-21(ok3427) III.
Alternate IDs: WB-STRAIN:VC2595, CGC_VC2595
Notes: Made_by: Vancouver KO Group|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK121.1. External left primer: AAATTGATTTTCAATGCCGC. External right primer: TCCGATGTTCGTGTTGCTTA. Internal left primer: TTCAGAAGTGTCTTGGCACG. Internal right primer: CTTTGAAGGCATCTCAACCG. Internal WT amplicon: 1154 bp. Deletion size: 470 bp. Deletion left flank: CTTAGCAAATTCTACAGTAATTCTTTAAAT. Deletion right flank: ACACATAAATCGTGGTAGGTGTGAAAACCT."
Proper citation: RRID:WB-STRAIN:WBStrain00037436 Copy
http://www.wormbase.org/db/get?name=WBStrain00037409
Source Database: WormBase (WB)
Genetic Background:
Affected Genes: WBGene00000254(bli-4)|WBGene00013557(pifk-1A)
Genomic Alteration: WBGene00000254(bli-4), WBGene00013557(pifk-1A)
Availability: available
References:
Synonyms: Y75B8A.24(ok3320) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2563, CGC_VC2563
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y75B8A.24. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok3320 homozygotes (grotty sterile with vulval blip). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CCTTCCCCTACCTAACAGCC. External right primer: GAGAAGGAAGTTGTCGGTGG. Internal left primer: ACAGAAGCTCATCTGCCGAG. Internal right primer: ACGTCGCATCCTACTCGTCT. Internal WT amplicon: 1206 bp. Deletion size: 389 bp. Deletion left flank: CTCATCTGCCGAGTAACTTCTCAGCACTCT. Deletion right flank: CCAACGCTAGCGGATGGAGCCAAGCACTGA."
Proper citation: RRID:WB-STRAIN:WBStrain00037409 Copy
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