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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pKOV Resource Report Resource Website 10+ mentions |
RRID:Addgene_25769 | Chloramphenicol | PMID:9335267 | pKOV is identical to the pKO3 vector described in J. Bacteriology 179: 6228-6237, except for the addition of a 3kb stuffer sequence in the multiple cloning site. This stuffer permits (i) directional cloning using NotI and BamHI and (ii) clean separation of doubly cut vector from singly cut contaminants when using this pair of enzymes. The pKOV cloning site is: 5' - SmaI - NotI - SmaI- stuffer - BamHI - SalI - 3'. BamHI and SalI are not used together, nor is SmaI used with NotI. BglII & BclI cut ends are compatible with BamHI. PmeI & SwaI are compatible with SmaI. NOTE: pKOV has a temperature sensitive pSC101 replication origin. To recover the plasmid, strains harboring the plasmid must be grown at 30 deg C under chloramphenicol selection. Gene replacement: Mutant alleles cloned into the pKOV gene replacement vector are electroporated into recombination proficient strains (eg. EMG2) and allowed to recover for 1 h at 30 deg C. The cells are plated on prewarmed chloramphenicol/LB plates and incubated at 42 deg C. To measure the integration frequency, the electroporated cells are also plated on chloramphenicol/LB plates at 30 deg C. From the 42 deg C plate, 1-5 colonies are picked into 1 ml of LB broth, serially diluted, and immediately plated at 30°ree;C on either 5% w/v sucrose or 5% sucrose+antibiotic plates. The 5% sucrose plates are replica plated to chloramphenicol plates at 30 deg C to test for loss of the replacement vector (cms). The gene replacement is confirmed by either PCR using primers flanking the targeted open reading frame or by genomic Southern's. Note from depositor: If sucrose plates do not select correctly, pKOV should be used in liquid selection on a plate reader with a LB + Cm control growth and a LB + Cm + sucrose growth. pKOV can sometimes still allow survival in the presence of sucrose, but significantly decreases fitness, so tracking the kinetic growth can be helpful. The sacB gene loses its efficacy,, sacB is toxic in E. coli even in the absence of sucrose. | Backbone Marker:N/A; Backbone Size:8673; Vector Backbone:pMAK700, pMAK705, pBS-TS; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:16:19 | 10 | |||
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pBTBXh-3 Resource Report Resource Website |
RRID:Addgene_26079 | Chloramphenicol | PMID:20148414 | There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function. | Backbone Size:3691; Vector Backbone:pBTBXh-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:16:21 | 0 | |||
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pBMTBX-3 Resource Report Resource Website |
RRID:Addgene_26074 | Chloramphenicol | PMID:20148414 | Backbone Size:4922; Vector Backbone:pBMTBX-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:16:21 | 0 | ||||
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pPSSUB-101 Resource Report Resource Website |
RRID:Addgene_26580 | destabilized GFP | Aequorea victoria | Chloramphenicol | PMID:15096621 | A novel hybrid promoter luxPRcI-OR1 (wild-type luxPR promoter with a CI OR1 operator site inserted at the +1 transcription start) drives expression of GFP(LVA). The promoter was constructed by encoding OR1 on PCR primers and inserting it after luxPR | Backbone Size:0; Vector Backbone:pLuxI-tet8 + pRKM-102; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | S2R, C-terminal extension RPAANDENYA(LVA) | Addgene | 2026-09-19 02:16:26 | 0 |
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pBB528 Resource Report Resource Website |
RRID:Addgene_27390 | lacIq | E. coli | Chloramphenicol | PMID:17565681 | Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli. de Marco A. Nat Protoc. 2007;2(10):2632-9. | Backbone Size:4945; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:16:34 | 0 | |
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pDE43-MCSq22 Resource Report Resource Website |
RRID:Addgene_27335 | qtag22 | Chloramphenicol | PMID:21203517 | Backbone Marker:N/A; Backbone Size:4522; Vector Backbone:pTCS-2M1-icl2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | N/A | Addgene | 2026-09-19 02:16:33 | 0 | ||
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pDE43-MCSq17 Resource Report Resource Website |
RRID:Addgene_27333 | qtag17 | Chloramphenicol | PMID:21203517 | Backbone Marker:N/A; Backbone Size:4522; Vector Backbone:pTCS-2M1-icl2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | N/A | Addgene | 2026-09-19 02:16:33 | 0 | ||
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pDE43-MCKq20 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27334 | qtag20 | Chloramphenicol | PMID:21203517 | Backbone Marker:N/A; Backbone Size:5740; Vector Backbone:pDE43-MCKq1; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol | N/A | Addgene | 2026-09-19 02:16:33 | 1 | ||
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pCK671.306 Resource Report Resource Website |
RRID:Addgene_192641 | J306 scRNA | Synthetic | Chloramphenicol | PMID:36378874 | Backbone Marker:Jesse Zalatan; Backbone Size:1726; Vector Backbone:pCK669 (Addgene #192638); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:37 | 0 | ||
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pCK341 Resource Report Resource Website |
RRID:Addgene_192640 | Sp.pCas9-dSpRY | S. pyogenes (dCas9) | Chloramphenicol | PMID:36378874 | Backbone Marker:Jesse Zalatan; Backbone Size:1726; Vector Backbone:pCD442 (Addgene #153024); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol | dSpRY has several mutations compared to dCas9 | Addgene | 2026-09-19 02:28:37 | 0 | |
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pCK668 Resource Report Resource Website |
RRID:Addgene_192637 | Sp.pCas9-dCas9-NG | S. pyogenes (dCas9) | Chloramphenicol | PMID:36378874 | Backbone Marker:Jesse Zalatan; Backbone Size:1726; Vector Backbone:pCD442 (Addgene #153024); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol | dCas9-NG has several mutations compared to dCas9 | Addgene | 2026-09-19 02:28:37 | 0 | |
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pUPD2_SulR(CDS) Resource Report Resource Website |
RRID:Addgene_186423 | SulR | Arabidopsis thaliana | Chloramphenicol | PMID:36609768 | Backbone Marker:GoldenBraid kit from Diego Orzaez (Addgene kit # 1000000076 ), IBMCP, Valencia, Spain; Backbone Size:2106; Vector Backbone:pUPD2; Vector Types:Plant Expression, Synthetic Biology, CDS (part B3-B4-B5), GoldenBraid level 0; Bacterial Resistance:Chloramphenicol | BsaI and BsmBI sites removed | Addgene | 2026-09-19 02:28:38 | 0 | |
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pAS157 Resource Report Resource Website |
RRID:Addgene_194480 | synGR (Part 3) | Synthetic | Chloramphenicol | PMID:36367334 | Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:39 | 0 | ||
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pAS169 Resource Report Resource Website |
RRID:Addgene_194492 | tCYC1 (Part 4) | Synthetic | Chloramphenicol | PMID:36367334 | Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:39 | 0 | ||
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pAS155 Resource Report Resource Website |
RRID:Addgene_194478 | synDHBR (Part 3) | Synthetic | Chloramphenicol | PMID:36367334 | Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:39 | 0 | ||
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GB2886 Resource Report Resource Website |
RRID:Addgene_193124 | GB_SynP (A2) G1ab.3 | Synthetic | Chloramphenicol | PMID:36044643 | Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:40 | 0 | ||
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pAS167 Resource Report Resource Website |
RRID:Addgene_194490 | 37-12 Op (Part 2) | Synthetic | Chloramphenicol | PMID:36367334 | Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:40 | 0 | ||
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pAS168 Resource Report Resource Website |
RRID:Addgene_194491 | Zif268 Op (Part 2) | Synthetic | Chloramphenicol | PMID:36367334 | Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:40 | 0 | ||
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GB2888 Resource Report Resource Website |
RRID:Addgene_193126 | GB_SynP (A2) G1ab.4 | Synthetic | Chloramphenicol | PMID:36044643 | Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | Addgene | 2026-09-19 02:28:42 | 0 | ||
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AT09404 Resource Report Resource Website |
RRID:DGRC_1024724 | Drosophila melanogaster | Chloramphenicol | PMID:12176937 | BDGP ESTs | Drosophila Genomics Resource Center | 2026-09-19 05:35:02 | 0 |
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