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URL: http://www.wormbase.org/db/get?name=WBStrain00036862
Proper Citation: RRID:WB-STRAIN:WBStrain00036862
Description: Caenorhabditis elegans with name apc-1(gk824)/mIn1 [mIs14 dpy-10(e128)] II. from WB.
Species: Caenorhabditis elegans
Synonyms: apc-1(gk824)/mIn1 [mIs14 dpy-10(e128)] II.
Notes: Mutagen:UV/TMP|"Supplementary_genotype mat-2(gk824)/mIn1 [mIs14 dpy-10(e128)] II."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"W10C6.1. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP gk824 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TATGTGGAACCGAAGGAAGG. External right primer: CAGGCGTTGTTCTTTCACAA. Internal left primer: GGTCTCAGTCACCGGAGAAG. Internal right primer: ATTCAATGACCAACACGGCT. Internal WT amplicon: 2186 bp. Deletion size: 1963 bp. Deletion left flank: TGGAAGAATGCGGAGACTACACGAAAAAAT. Deletion right flank: AAAGTTATGAATTATCGTGCATTCGAGGAA.. Formerly known as mat-2."
Affected Gene: WBGene00001072(dpy-10)|WBGene00003133(apc-1)
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Source: Integrated Animals
Source Database: WormBase (WB)
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