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URL: http://www.wormbase.org/db/get?name=WBStrain00034464
Proper Citation: RRID:WB-STRAIN:WBStrain00034464
Description: Caenorhabditis elegans with name rad-51(iow53[GFP::rad-51]) IV/nT1[qIs51] (IV;V). from WB.
Species: Caenorhabditis elegans
Synonyms: rad-51(iow53[GFP::rad-51]) IV/nT1[qIs51] (IV;V).
Notes: Heterozygotes are wild-type with pharyngeal-expressed GFP, and segregate wild-type GFP+(pharynx) heterozygotes, arrested nT1[qIs51] homozygotes, and viable non-GFP(pharynx) rad-51(iow53[GFP::rad-51]) homozygotes. Balancer is prone to breaking down. If a population contains a mix of bright and dim GFP animals, pick dim GFP and check for correct segregation of progeny to maintain. iow53 inserted a GFP tag at the N-terminus of the endogenous rad-51 locus, but the tagged protein is not fully functional. non-GFP(pharynx) rad-51(iow53[GFP::rad-51]) homozygotes form GFP foci in the germline that are mostly spo-11 dependent, and GFP::rad-51 homozygotes have defects in unloading RAD-51. Created by CRISPR using pDD282, therefore may also contain 3XFLAG. Reference: Koury E, et al. Nucleic Acids Res. 2018 Jan 25;46(2):748-764.|"Made_by: Matthew Wheat and Emily Koury"
Affected Gene: WBGene00004297(rad-51)
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Source: Integrated Animals
Source Database: WormBase (WB)
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