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URL: http://www.wormbase.org/db/get?name=WBStrain00034461
Proper Citation: RRID:WB-STRAIN:WBStrain00034461
Description: Caenorhabditis elegans with name mre-11(iow1)/nT1[qIs51] (IV;V). from WB.
Species: Caenorhabditis elegans
Synonyms: mre-11(iow1)/nT1[qIs51] (IV;V).
Notes: Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP, arrested nT1[qIs51] aneuploids, and non-GFP iow1 homozygotes (viable; see note below). Homozygous nT1[qIs51] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. iow1 is a separation-of-function allele of mre-11. Homozygotes develop into adults wild-type in appearance, but laying dead eggs due to defects in meiotic DSB repair: mre-11(iow1) worms form meiotic DSBs but are impaired in their resection. Pick GFP+ to maintain (mre-11(iow1)/nT1[qIs51]) and GFP- to obtain homozygous mre-11(iow1) worms for analysis. Reference: Yin Y & Smolikove S. Mol Cell Biol. 2013 Jul;33(14):2732-47. doi: 10.1128/MCB.00055-13. Epub 2013 May 13. Erratum in: Mol Cell Biol. 2015 Jul;35(14):2568. PubMed PMID: 23671188; PubMed Central PMCID: PMC3700128.|"Mutagen:UV/TMP"
Affected Gene: WBGene00003405(mre-11)
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Source: Integrated Animals
Source Database: WormBase (WB)
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