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University of California Davis DNA Technologies and Expression Analysis Core Facility (RRID:SCR_028316)Copy Citation Copied
URL: http://dnatech.ucdavis.edu
Proper Citation: University of California Davis DNA Technologies and Expression Analysis Core Facility (RRID:SCR_028316)
Description: Core offers high-throughput sequencing, genotyping, single-cell multi-omics, and spatial biology services as well as training and consultation. Next Generation Sequencing (NGS) technologies including Illumina and Element Biosciences short-read sequencing, PacBio and Nanopore long-read sequencing services provide sequencing and library preparation for these platforms. Genotyping is performed on the Fluidigm EP1 System for low to medium assay numbers, genotyping-by-sequencing assays, and with a range of genotyping-by-sequencing technologies (e.g. multiplexed amplicon, single-primer extension, reduced representation, and skim-seq protocols). Gene expression analysis is carried out by RNA-seq on short-read sequencers as well as full-length transcript sequencing on long-read systems. Single-cell transcriptome (high-throughput single-cell gene expression profiling) and multi-ome studies are enabled by our 10X Genomics Chromium X system and Parse Biosciences assays. Spatial transcriptome profiling is supported on the Xenium and Visium HD platforms with the help of our CytAssist instrument for fresh-frozen, fixed-frozen, and FFPE tissue sections. We offer annual Illumina and PacBio sequencing library preparation workshops, free consultations on project considerations and experimental design, and custom sequencing library prep solutions.
Synonyms: DNA Technologies and Expression Analysis Core
Resource Type: core facility, service resource, access service resource
Keywords: ABRF, sequencing, genotyping, single-cell multi-omics, spatial biology, services and training,
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